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陈海南,卢园,王兰兰,孙梦龙,薛惠天,杨舟,彭亮.按法对脑卒中肌痉挛大鼠脊髓内5-HT2B受体、5-HT2C受体表达与GABA含量的影响[J].湖南中医药大学学报英文版,2022,42(7):1169-1174.[Click to copy
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| 按法对脑卒中肌痉挛大鼠脊髓内5-HT2B受体、5-HT2C受体表达与GABA含量的影响 |
| 陈海南,卢园,王兰兰,孙梦龙,薛惠天,杨舟,彭亮 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察按法对脑卒中肌痉挛大鼠脊髓内5-羟色胺2B（5-hydroxytryptamine 2B,5-HT2B）受体和5-羟色胺2C（5-hydroxytryptamine 2C,5-HT2C）受体表达水平与γ-氨基丁酸（γ－aminobutyric acid,GABA）含量变化的影响。方法 32只大鼠采用随机数字表法均分为4组，分别是空白组、假手术组、模型组和治疗组，模型组和治疗组构造MCAO脑缺血模型，假手术组进行与模型组相同的手术操作但不插入线栓。后通过改良Ashworth法测定大鼠的肌张力水平，平衡木实验测定大鼠的运动功能，Western blot法检测脊髓中5-HT2B受体和5-HT2C受体表达情况，ELISA法检测脊髓中GABA含量。结果 治疗后，改良Ashworth肌张力评分方面，模型组、治疗组明显高于空白组和假手术组（P<0.05），而治疗组肌张力水平显著低于模型组（P<0.05）；平衡木运动能力评分方面，模型组、治疗组评分均显著低于空白组和假手术组（P<0.05），而治疗组评分显著高于模型组（P<0.05）；模型组、治疗组5-HT2B受体和5-HT2C受体表达量相比于空白组和假手术组有明显提升（P<0.05），且治疗组中5-HT2B受体和5-HT2C受体表达量显著低于模型组（P<0.05）；模型组GABA含量低于空白组和假手术组（P<0.05），且治疗组GABA含量高于模型组（P<0.05）。结论 按法可以有效缓解脑卒中后大鼠的肌痉挛、降低肌张力、提升运动功能恢复速度，其机制可能与脊髓中的5-HT2B受体和5-HT2C受体的表达以及GABA释放量有关。 |
| 关键词: 脑卒中 按法 肌痉挛 5羟色胺受体 γ-氨基丁酸 改良Ashworth肌张力评分 平衡木运动能力评分 |
| DOI：10.3969/j.issn.1674-070X.2022.07.017 |
| Received:December 21, 2021 |
| 基金项目:国家自然科学基金项目（82174521）；湖南省教育厅优秀青年项目（19B438）。 |
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| Effect of the pressing manipulation on the expression of 5-HT2B receptor and 5-HT2C receptor and GABA content in the spinal cord of rats with cerebral apoplexy muscle spasm |
| CHEN Hainan,LU Yuan,WANG Lanlan,SUN Menglong,XUE Huitian,YANG Zhou,PENG Liang |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of the pressing manipulation on the expression levels of 5-hydroxytryptamine 2B (5-HT2B) receptor and 5-hydroxytryptamine 2C (5-HT2C) receptor and the changes of γ-aminobutyric acid (GABA) content in the spinal cord of rats with cerebral apoplexy muscle spasm. Methods A total of 32 rats were divided into 4 groups by random number table method, namely blank group, sham operation group, model group and treatment group. The model group and treatment group constructed the MCAO cerebral ischemia model, and the sham operation group performed the same operation as the model group without inserting the suture. The muscle tension level of the rats was measured by the modified Ashworth muscle tension score, the motor function of the rats was measured by the balance beam experiment, the expression of 5-HT2B receptor and 5-HT2C receptor in the spinal cord was measured by Western blot, and the GABA content in the spinal cord was measured by ELISA. Results After treatment, the modified Ashworth muscle tension score in the model group and the treatment group was significantly higher than that in the blank group and the sham operation group (P<0.05), while the muscle tension level in the treatment group was significantly lower than that in the model group (P<0.05); in terms of balance beam exercise ability score, the scores of the model group and the treatment group were significantly lower than those of the blank group and the sham operation group (P<0.05), while the score of the treatment group was significantly higher than that of the model group (P<0.05); the expressions of 5-HT2B receptor and 5-HT2C receptor in the model group and the treatment group were significantly higher than those in the blank group and the sham operation group (P<0.05), and the expression levels of 5-HT2B receptor and 5-HT2C receptor in the treatment group were significantly lower than those in the model group (P<0.05); the content of GABA in the model group was lower than that in the blank group and the sham operation group (P<0.05), and the content of GABA in the treatment group was higher than that in the model group (P<0.05). Conclusion Pressing manipulation can effectively relieve muscle spasm of rats after cerebral apoplexy, reduce muscle tension, and improve the recovery speed of motor ability. The mechanism may be related to the expression of 5-HT2B receptor and 5-HT2C receptor in spinal cord and the release of GABA. |
| Key words: cerebral apoplexy pressing manipulation muscle spasm 5 hydroxytryptamine receptor γ-aminobutyric acid modified Ashworth muscle tension score balance beam exercise ability score |
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