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陈成,刘毅,许汉林.土田七醇提物抑制肝癌细胞增殖活性部位筛选及其对肝癌细胞侵袭迁移的影响[J].湖南中医药大学学报英文版,2022,42(6):930-933.[Click to copy
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| 土田七醇提物抑制肝癌细胞增殖活性部位筛选及其对肝癌细胞侵袭迁移的影响 |
| 陈成,刘毅,许汉林 |
| (湖北中医药大学, 湖北 武汉 430065) |
| 摘要: |
| 目的 筛选土田七醇提物抗肝癌活性部位，并研究其对肝癌SMMC-7721细胞和HepG2细胞侵袭迁移的影响。方法 采用大孔树脂对土田七醇提物进行洗脱，并采用CCK-8法检测不同洗脱部位对肝癌细胞增殖的影响；建立荷瘤小鼠模型，通过瘤体质量变化评价不同洗脱部位的体内抗肿瘤作用；采用Transwell侵袭实验和细胞划痕实验检测肝癌细胞侵袭迁移能力。结果 土田七醇提物的大孔树脂不同洗脱部位均有抑制肝癌细胞增殖的作用，并可抑制裸鼠肿瘤的生长，其中70%洗脱部位的效果最好。Transwell侵袭实验和细胞划痕实验结果显示，70%洗脱部位可抑制肝癌细胞侵袭和迁移过程。结论 土田七醇提物的大孔树脂70%洗脱部位抗肝癌活性最好，并可抑制肝癌细胞侵袭迁移能力。 |
| 关键词: 土田七 肝癌 醇提物 活性部位 侵袭 迁移 |
| DOI：10.3969/j.issn.1674－070X.２０22.06.010 |
| Received:December 21, 2021 |
| 基金项目:湖北中医药大学"青苗计划"项目(2017ZZX002)。 |
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| Screening of active fractions from the alcohol extract of Stahlianthus involucratus and its effect on invasion and migration of hepatocellular carcinoma cells |
| CHEN Cheng,LIU Yi,XU Hanlin |
| (Hubei University of Chinese Medicine, Wuhan, Hubei 430065, China) |
| Abstract: |
| Objective To screen the anti-hepatocellular carcinoma active fractions from the alcohol extract of Stahlianthus involucratus, and investigate its effect on invasion and migration of SMMC-7721 and HepG2. Methods The alcohol extract of Stahlianthus involucratus was eluted by macroreticular adsorbents, followed by the CCK-8 to investigate the hepatocellular carcinoma cell proliferation treated by the different fractions. The tumor bearing mouse model was established and used to screen the antitumor effect in vivo with different fractions by the changes of the weight of tumor mass. The invasion and migration abilities of hepatocellular carcinoma cells were detected by Transwell chamber assay and scratch assay. Results Different fractions of macroreticular adsorbents from alcohol extract of Stahlianthus involucratus can inhibit the multiplication of hepatocellular carcinoma cells and inhibit the growth of tumor in nude mice. Among them, the fraction eluted with 70% ethanol has the best effect. Both Transwell chamber assay and scratch assay results showed that the fraction eluted with 70% ethanol can inhibit the migration and invasion of hepatocellular carcinoma cells. Conclusion The 70% elution fraction of macroreticular adsorbents from alcohol extract of Stahlianthus involucratus has the best anti-liver cancer activity, which also could inhibit the invasion and migration of hepatocellular carcinoma cells. |
| Key words: Stahlianthus involucratus hepatocellular carcinoma alcohol extract active fraction invasion migration |
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