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黄钧伟,马壮壮,齐同飞,邓晗薇,杨伟丽,朱镇华.玉屏风散对肺气虚型变应性鼻炎豚鼠血清中TSLP及相关因子的影响[J].湖南中医药大学学报英文版,2022,42(6):906-910.[Click to copy
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| 玉屏风散对肺气虚型变应性鼻炎豚鼠血清中TSLP及相关因子的影响 |
| 黄钧伟,马壮壮,齐同飞,邓晗薇,杨伟丽,朱镇华 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 观察玉屏风散对肺气虚型变应性鼻炎豚鼠炎症反应的治疗效果及其机制。方法 将40只雄性豚鼠分为空白组、模型组、氯雷他定组及玉屏风散组，每组10只。通过烟熏+卵清蛋白注射致敏加滴鼻激发的方法，诱导建立肺气虚型变应性鼻炎模型。玉屏风散组每日给予4.52 g/kg玉屏风散，氯雷他定组每日给予0.903 mg/kg氯雷他定片，空白组与模型组给予等体积的0.9%氯化钠溶液。用药治疗15 d后，采用叠加量化法测定豚鼠用药前后鼻部症状评分；取鼻黏膜组织进行HE染色；采用ELISA法检测血清IgE、IL-4、IL-13、TSLP水平。结果 HE染色结果显示，模型组豚鼠鼻腔黏膜固有层出现大量淋巴细胞和嗜酸性粒细胞浸润，腺体增生和肥大，血管扩张和充血；玉屏风散组和氯雷他定组豚鼠鼻黏膜组织相较模型组病理状况有所改善，炎性细胞浸润明显减轻，腺体增生少，结构基本完整。治疗前，与空白组相比，模型组鼻部症状评分明显升高（P＜0.01），血清IgE、IL-4、IL-13、TSLP表达水平均显著上升（P＜0.01）；治疗后，玉屏风散组和氯雷他定组豚鼠相较于模型组的鼻部症状评分显著降低（P<0.01），血清IgE、IL-4、IL-13、TSLP表达水平降低（P<0.05）。结论 玉屏风散可明显缓解肺气虚型变应性鼻炎豚鼠症状，其途径可能是通过降低血清中TSLP的含量，抑制相关炎症因子表达，进而减轻鼻部症状。 |
| 关键词: 变应性鼻炎 玉屏风散 中医 氯雷他定 Th1/Th2平衡 |
| DOI：10.3969/j.issn.1674－070X.２０22.06.006 |
| Received:March 05, 2022 |
| 基金项目:国家自然科学基金面上项目(81373699);湖南省自然科学基金项目(2020JJ44)。 |
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| Effect of Yupingfeng Powder on TSLP and related factors in the serum of guinea pigs model with allergic rhinitis with lung Qi deficiency syndrome |
| HUANG Junwei,MA Zhuangzhuang,QI Tongfei,DENG Hanwei,YANG Weili,ZHU Zhenhua |
| (Hunan Umiversity of Chinese Medicine, Changsha, Hunan 410208, China;The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China)) |
| Abstract: |
| Objective To explore the therapeutic effect and mechanism of Yupingfeng Powder on inflammatory response of allergic rhinitis guinea pigs with lung Qi deficiency syndrome. Methods Forty male guinea pigs were randomly divided into the blank group, the model group, the Yupingfeng Powder group and the loratadine group (n=10). The allergic rhinitis model of lung Qi deficiency syndrome was established by smoking+ovalbumin injection sensitization and nasal instillation stimulation, Yupingfeng Powder group was given 4.52 g/kg Yupingfeng Powder per day, loratadine group was given 0.903 mg/kg loratadine tablets per day, blank group and model group were given an equal volume of 0.9% sodium chloride solution. After 15 d of treatment, the nasal symptom score of guinea pigs before and after treatment was measured by superposition quantitative method; the nasal mucosa was taken for HE staining; the levels of serum IgE, IL-4, IL-13 and TSLP were detected by ELISA. Results HE staining results showed that a large number of lymphocytes and eosinophils infiltrated, nasal gland hyperplasia and hypertrophy, vasodilation and hyperemia appeared in the model group. The nasal mucosal tissue of guinea pigs in the Yupingfeng Powder group and loratadine group improved compared with the pathological status of the model group, the inflammatory cell infiltration was significantly reduced, the glandular hyperplasia was small, and the structure was basically complete. Before treatment, compared with the blank group, the nasal symptom score of the model group was significantly increased (P<0.01), and the serum IgE, IL-4, IL-13, and TSLP levels were significantly increased (P<0.01). After treatment, nasal symptom scores of the Yupingfeng Powder group and loratadine group were significantly lower (P<0.01) and the levels of serum IgE, IL-4, IL-13, and TSLP were lower (P<0.05) compared with the model group. Conclusion Yupingfeng Powder can effectively improve the symptoms of lung Qi deficiency guinea pigs with allergic rhinitis, which may be achieved by reducing the content of TSLP in serum, inhibiting the expression of related inflammatory factors, and then alleviating nasal symptoms. |
| Key words: allergic rhinitis Yupingfeng Powder traditional Chinese medicine loratadine balance of Th1/Th2 |
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