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王旭,周旭晴,吴笛,陈姿霖,蔺晓源,夏旭婷,郭纯.决明二仁方对不同便秘模型小鼠排便及血清VIP、SP的影响[J].湖南中医药大学学报英文版,2022,42(5):732-737.[Click to copy
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| 决明二仁方对不同便秘模型小鼠排便及血清VIP、SP的影响 |
| 王旭,周旭晴,吴笛,陈姿霖,蔺晓源,夏旭婷,郭纯 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察决明二仁方对3种便秘模型小鼠的排便及血清血管活性肠肽（vasoactive intestinal polypeptide， VIP）、P物质（substance P，SP）的影响。方法 复制燥结失水、脾虚、复方地芬诺酯3种便秘小鼠模型，每一模型设空白组、模型组、麻仁软胶囊组和决明二仁方低、中、高剂量组，每组6只。造模成功后，模型组给予纯净水，治疗组分别给予麻仁软胶囊（0.312 g/mL）和决明二仁方低（0.208 g/mL）、中（0.416 g/mL）、高（0.832 g/mL）剂量药液。给药14 d后，观察各组小鼠的首粒黑便排出时间及4 ｈ内排便粒数，ELISA检测小鼠血清中VIP、SP含量。结果 与同一模型的空白组比较，3种模型的模型组小鼠体质量、4 h排便粒数、血清VIP和SP浓度明显降低，首粒黑便时间明显增加(P<0.05或P<0.01)。与同一模型的模型组比较，各给药组的小鼠首粒黑便时间均明显降低(P<0.05或P<0.01)；与燥结失水便秘模型组比较，各用药组的小鼠体质量、血清SP浓度均升高，决明二仁方高剂量组的4 h排便粒数和决明二仁方中、高剂量组血清VIP浓度也明显升高(P<0.05或P<0.01)；与脾虚便秘模型组比较，各用药组小鼠的4 h排便粒数、血清VIP和SP浓度升高，决明二仁方中、高剂量组小鼠体质量也明显升高(P<0.05或P<0.01)；与复方地芬诺酯便秘模型组比较，各用药组小鼠体质量、血清VIP和SP浓度升高，决明二仁方中、高剂量组的4 h排便粒数也明显升高(P<0.05或P<0.01)。结论 决明二仁方对3种便秘模型小鼠均具有良好的促排便作用，其作用可能与升高血清VIP、SP水平有关。 |
| 关键词: 决明二仁方 便秘 燥结失水 脾虚 复方地芬诺酯 血管活性肠肽 P物质 |
| DOI：10.3969/j.issn.1674-070X.2022.05.007 |
| Received:November 22, 2021 |
| 基金项目:国家自然科学基金项目（81603597)；长沙市科技计划项目（kq1907036）。 |
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| Effect of Jueming Erren Formula on defecation and serum VIP and SP in mice with different constipation models |
| WANG Xu,ZHOU Xuqing,WU Di,CHEN Zilin,LIN Xiaoyuan,XIA Xuting,GUO Chun |
| (The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of the Jueming Erren Formula on defecation and serum vasoactive intestinal polypeptide (VIP), substance P (SP) of mice with different constipation models. Methods Three constipation models with dry stagnation and water loss, spleen deficiency and compound diphenoxylate were established. In each model, a blank group, a model group, a Maren soft capsule group, a Jueming Erren Formula low-dose group, a Jueming Erren Formula medium-dose group and a Jueming Erren Formula high-dose group were further established, with 6 mice in each group. After successful modeling, the model group was given pure water, and the treatment groups were given Maren soft capsule and Jueming Erren Formula low-dose (0.208 g/mL), medium-dose (0.416 g/mL), high-dose (0.832 g/mL) liquid medicine, respectively. After 14 days of drug administration, the excretion time of the first black stool and the defecation grain number within 4 h were observed, and the serum content levels of VIP and SP were detected by ELISA. Results Compared with the blank group of the same model, the body weight, 4 h defecation grain number, serum VIP and SP concentrations of the mice in the model groups were significantly reduced, and the first black stool time was significantly increased (P<0.05 or P<0.01). Compared with the model group of the same model, the first black stool time in each administration group was significantly decreased (P<0.05 or P<0.01). Compared with the dry stagnation and water loss constipation model group, the body weight and serum SP concentration of mice in each administration group were increased, and the 4 h defecation grain number in Jueming Erren Formula high-dose group and serum VIP concentration in Jueming Erren Formula medium-dose and high-dose groups were also significantly increased (P<0.05 or P<0.01). Compared with the spleen deficiency constipation model group, the 4 h defecation grain number, serum VIP and SP concentrations of mice in each administration group were increased, and the body weight of mice in the Jueming Erren Formula medium-dose and high-dose groups were also significantly increased (P<0.05 or P<0.01). Compared with the compound diphenoxylate model group, the body weight, serum VIP and SP concentrations of mice in each administration group were increased, and the 4 h defecation grain numbers in the Jueming Erren Formula medium-dose and high-dose groups were also significantly increased (P<0.05 or P<0.01). Conclusion Jueming Erren Formula has good function of promoting defecation on mice with the three kinds of constipation models, which may be related to the increase of serum VIP and SP levels. |
| Key words: Jueming Erren Formula constipation dry stagnation and water loss spleen deficiency compound diphenoxylate vasoactive intestinal peptide substance P |
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