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史雯,陈信义,田劭丹,姚靓,何昊.黄连汤与加味黄连汤对小鼠胰腺癌移植瘤的抑制作用[J].湖南中医药大学学报英文版,2022,42(4):576-582.[Click to copy
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| 黄连汤与加味黄连汤对小鼠胰腺癌移植瘤的抑制作用 |
| 史雯,陈信义,田劭丹,姚靓,何昊 |
| (北京中医药大学东直门医院, 北京 100700;北京市第六医院, 北京 100007;西安医学院药学院, 陕西 西安 710021) |
| 摘要: |
| 目的 观察黄连汤、加味黄连汤对胰腺癌移植瘤模型小鼠体质量、游泳时间、肿瘤体积、肿瘤抑制率的影响。方法 将小鼠胰腺癌细胞接种于BALB/c小鼠右前肢腋窝下，构建胰腺癌移植瘤模型。造模后将84只小鼠随机分为7组：模型对照组（0.01 mL/g），黄连汤中剂量组（5.98 mg/g）、黄连汤高剂量组（11.96 mg/g）、加味黄连汤中剂量组（8.32 mg/g）、加味黄连汤高剂量组（16.64 mg/g）、吉西他滨中剂量组（0.25 mg/g）、吉西他滨高剂量组（0.50 mg/g），每组12只。模型对照组每日给予生理盐水灌胃，黄连汤中、高剂量组给予相应剂量黄连汤灌胃，加味黄连汤中、高剂量组给予相应剂量加味黄连汤灌胃，吉西他滨中、高剂量组皮下注射相应剂量注射用盐酸吉西他滨，各组均连续干预14 d。实验过程中和实验结束后，观察各组小鼠体质量、游泳时间、肿瘤体积、肿瘤抑制率。结果 模型对照组、黄连汤中剂量组、黄连汤高剂量组第4、8、12天，加味黄连汤高剂量组第8、12天，吉西他滨高剂量组第4、8天肿瘤体积均明显大于给药前（P<0.05）。黄连汤中、高剂量组第12天，加味黄连汤高剂量组第8天肿瘤体积均明显大于第4天（P<0.05）。模型对照组、黄连汤中剂量组、加味黄连汤中剂量组第12天肿瘤体积均明显大于第8天（P<0.05）。吉西他滨高剂量组第12天肿瘤体积明显小于第8天（P<0.05）。黄连汤中剂量组、黄连汤高剂量组、加味黄连汤中剂量组第4、8、12天，加味黄连汤高剂量组第12天体质量均明显大于给药前（P<0.05）。加味黄连汤中、高剂量组第12天体质量均明显大于第8天（P<0.05）。吉西他滨中、高剂量组第12天体质量均明显小于第4、8天（P<0.05）。黄连汤中剂量组、加味黄连汤中剂量组、加味黄连汤高剂量组第7、14天，黄连汤高剂量组、吉西他滨中剂量组、吉西他滨高剂量组第14天游泳时间均明显短于给药前（P<0.05）。模型对照组第14天游泳时间明显短于第7天（P<0.05）。第12天，黄连汤高剂量组、加味黄连汤高剂量组体质量、肿瘤体积均明显大于吉西他滨高剂量组（P<0.05）；黄连汤中剂量组、加味黄连汤中剂量组体质量均明显大于吉西他滨中剂量组（P<0.05）。第14天，黄连汤高剂量组、加味黄连汤高剂量组游泳时间均明显长于吉西他滨高剂量组（P<0.05）；黄连汤中剂量组、加味黄连汤中剂量组游泳时间均明显长于吉西他滨中剂量组（P<0.05）。加味黄连汤高剂量、吉西他滨中剂量组、吉西他滨高剂量组肿瘤抑制率均明显高于模型对照组（P<0.05，P<0.01）。结论 高剂量的加味黄连汤抑制小鼠胰腺癌移植瘤的效果与吉西他滨相近，虽然在缩小肿瘤体积方面不如吉西他滨，但在增加体质量及延长游泳时间方面明显优于吉西他滨。而黄连汤仅在增加体质量及延长游泳时间方面优于吉西他滨。 |
| 关键词: 胰腺癌 黄连汤 加味黄连汤 胰腺癌移植瘤 肿瘤抑制率 吉西他滨 疏肝健脾 平调寒热 |
| DOI：10.3969/j.issn.1674－070X.2022.04.010 |
| Received:January 10, 2022 |
| 基金项目:北京市科学技术委员会科技计划课题（D131100002213002）。 |
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| Inhibitory effect of Huanglian Decoction and Jiawei Huanglian Decoction on mouse pancreatic cancer transplanted tumor |
| SHI Wen,CHEN Xinyi,TIAN Shaodan,YAO Liang,HE Hao |
| (Dongzhimen Hospital of Beijing University of Chinese Medcine, Beijing 100700, China;The Sixth Hospital of Beijing, Beijing 100007, China;School of Pharmacy, Xi'an Medical University, Xi'an, Shaanxi 710021, China) |
| Abstract: |
| Objective To observe the effects of Huanglian Decoction and Jiawei Huanglian Decoction on the body weight, swimming time, tumor volume and tumor inhibition rate of pancreatic cancer transplanted tumor model mice. Methods Mouse pancreatic cancer cells were inoculated under the armpit of the right forelimb of BALB/c mice to construct a pancreatic cancer transplanted tumor model. After tumor formation, 84 mice were randomly divided into 7 groups: model control group (0.01 mL/g), Huanglian Decoction medium-dose group (5.98 mg/g), Huanglian Decoction high-dose group (11.96 mg/g), Jiawei Huanglian Decoction medium-dose group (8.32 mg/g), Jiawei Huanglian Decoction high-dose group (16.64 mg/g), gemcitabine medium-dose group (0.25 mg/g) and gemcitabine high-dose group (0.50 mg/g), with 12 mice in each group. The model control group was given normal saline intragastric administration daily, the medium-dose and high-dose groups of Huanglian Decoction were given corresponding doses of Huanglian Decoction, the medium-dose and high-dose groups of Jiawei Huanglian Decoction were given corresponding doses of Jiawei Huanglian Decoction intragastric administration, and the medium-dose and high-dose gemcitabine groups were subcutaneously injected with appropriate doses of gemcitabine hydrochloride for injection, and the intervention lasted for 14 days. During and after the experiment, the body weight, swimming time, tumor volume and tumor inhibition rate of each group were observed. Results The tumor volume of model control group, medium-dose and high-dose groups of Huanglian Decoction on the 4th, 8th and 12th day, Jiawei Huanglian Decoction high-dose group on the 8th and 12th day, and gemcitabine high-dose group on the 4th and 8th day were significantly higher than before (P<0.05). The tumor volume of medium-dose and high-dose groups of Huanglian Decoction on the 12th day and Jiawei Huanglian Decoction high-dose group on the 8th day were significantly higher than that on the 4th day (P<0.05). The tumor volume of model control group, Huanglian Decoction medium-dose group and Jiawei Huanglian Decoction medium-dose group on the 12th day was significantly higher than that on the 8th day (P<0.05). The tumor volume of gemcitabine high-dose group on the 12th day was significantly lower than that on the 8th day (P<0.05). The body weight in medium-dose and high-dose groups of Huanglian Decoction, Jiawei Huanglian Decoction medium-dose group on the 4th, 8th and 12th day and Jiawei Huanglian Decoction medium-dose group on the 12th day were significantly higher than before (P<0.05). The body weight on the 12th day in the medium-dose and high-dose groups of Jiawei Huanglian Decoction was significantly higher than that on the 8th day (P<0.05). In medium-dose and high-dose groups of gemcitabine, the body weight on the 12th day was significantly lower than that on the 4th day and 8th day (P<0.05). The swimming time of the Huanglian Decoction medium-dose group, the medium-dose and high-dose groups of Jiawei Huanglian Decoction on the 7th and 14th day, the Huanglian Decoction high-dose group, the medium-dose and high-dose groups of gemcitabine on the 14th day were significantly shorter than before (P<0.05). The swimming time of model control group on the 14th day was significantly shorter than that on the 7th day (P<0.05). On the 12th day, body weight and tumor volume of Huanglian Decoction high-dose group and Jiawei Huanglian Decoction high-dose group were significantly higher than those of gemcitabine high-dose group (P<0.05); the body weight of the Huanglian Decoction medium-dose group and Jiawei Huanglian Decoction medium-dose group was significantly higher than that of gemcitabine medium-dose group (P<0.05). On the 14th day, the swimming time of Huanglian Decoction high-dose group and Jiawei Huanglian Decoction high-dose group was significantly longer than that of gemcitabine high-dose group (P<0.05); the swimming time of Huanglian Decoction medium-dose group and Jiawei Huanglian Decoction medium-dose group was significantly longer than that of gemcitabine medium-dose group (P<0.05). The tumor inhibition rate of Jiawei Huanglian Decoction high-dose, medium-dose and high-dose groups of gemcitabine were significantly higher than that of model control group (P<0.05, P<0.01). Conclusion The inhibitory effect of high-dose Jiawei Huanglian Decoction on transplanted mouse pancreatic cancer is similar to gemcitabine. Although it is not as effective as gemcitabine in reducing tumor volume, it is superior to gemcitabine in increasing body weight and prolonging swimming time. Huanglian Decoction is superior to gemcitabine only in increasing body weight and prolonging swimming time. |
| Key words: pancreatic cancer Huanglian Decoction Jiawei Huanglian Decoction pancreatic cancer transplanted tumor tumor inhibition rate gemcitabine soothing liver and strengthening spleen moderating cold and heat |
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