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王珊珊,李卫红,仁青加,李腾辉,解宇晴,李雅璇,杜庆红.藏药七味铁屑胶囊对CCL4大鼠肝纤维化程度的影响[J].湖南中医药大学学报英文版,2022,42(2):223-228.[Click to copy
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| 藏药七味铁屑胶囊对CCL4大鼠肝纤维化程度的影响 |
| 王珊珊,李卫红,仁青加,李腾辉,解宇晴,李雅璇,杜庆红 |
| (北京中医药大学中医学院, 北京 100029;西藏藏医药大学, 西藏 拉萨 850000;北京中医药大学东直门医院, 北京 100700) |
| 摘要: |
| 目的 观察藏药七味铁屑胶囊对CCL4诱导的肝纤维化大鼠肝纤维化程度的影响。方法 健康雄性SD大鼠60只，分为空白组（N组）、模型组（M组）、鳖甲软肝片组（Y组），以及七味铁屑胶囊高剂量组（QH组）、中剂量组（QM组）、低剂量组（QL组），每组10只。除N组外，其余大鼠建立大鼠CCL4肝纤维化模型。造模4周后，测定肝脾指数；全自动生化仪检测血清天冬氨酸氨基转移酶（aspartateaminotransferase，AST）、丙氨酸氨基转移酶（alanineaminotransferase，ALT）含量；采用ELISA法检测肝组织肿瘤坏死因子-α（tumornecrosisfactor，TNF-α）、白细胞介素-1β（interleukin，IL-1β）水平；定量检测肝组织羟脯氨酸（hydroxyproline，HYP）含量和谷胱甘肽过氧化物酶（glutathioneperoxidase，GSH-Px）活力。结果 与N组相比，M组肝脾指数明显升高（P<0.001）；AST、ALT、HYP含量显著上升（P<0.001）；TNF-α、IL-1β含量明显升高（P<0.05，P<0.01），GSH-Px活力显著降低（P<0.001）。与M组相比，各给药组肝脏指数均显著降低（P<0.01，P<0.001）；QM组脾脏指数显著降低（P<0.01）；Y组、QH组血清AST、ALT含量明显降低（P<0.05）；Y组、QH组、QM组HYP含量显著降低（P<0.01，P<0.001），GSH-Px活力升高（P<0.01，P<0.001）；Y组、QM组TNF-α水平明显降低（P<0.05），各治疗组IL-1β水平均显著下降（P<0.01，P<0.001）；与Y组比，QH组GSH-Px活力明显升高（P<0.05）。结论 藏药七味铁屑胶囊可减轻CCL4诱导的大鼠肝纤维化程度，其机制可能与缓解炎症反应、抑制胶原生成、增强抗氧化能力有关。 |
| 关键词: 藏药 七味铁屑胶囊 肝纤维化 炎症 抗氧化 |
| DOI：10.3969/j.issn.1674-070X.2022.02.009 |
| Received:May 24, 2021 |
| 基金项目:西藏自治区重点研发计划项目（XZ202001ZY0001G）；西藏自治区藏医药管理局级科技专项（JJKT2020006）；西藏自治区教育厅项目（2021ZYDKY001）；西藏藏医药大学2019年中医学（藏医）博士点建设项目（XZ202001ZY0001G）。 |
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| Effect of Tibetan medicine Qiwei Tiexie Capsule on the degree of liver fibrosis rats induced by CCL4 |
| WANG Shanshan,LI Weihong,REN Qingjia,LI Tenghui,XIE Yuqing,LI Yaxuan,DU Qinghong |
| (School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;University of Tibetan Medicine, Lasa, Tibetan 850000, China;Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China) |
| Abstract: |
| Objective To observe the effect of Qiwei Tiexie Capsule on the degree of liver fibrosis in CCL4-induced liver fibrosis rats. Methods 60 healthy male SD rats were divided into blank group (N group), model group (M group), Biejia Ruangan Tablet group (Y group), high-dose (QH), medium-dose (QM) and low-dose (QL) groups of Qiwei Tiexie Capsule, with 10 rats in each group. The CCL4 liver fibrosis model of rats was established except N group. After 4 weeks of modeling, the liver and spleen indexes were measured; the content of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) was detected by automatic biochemical analyzer; the levels of tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β) in liver tissues were detected by ELISA the content of hydroxyproline (HYP) and the activity of glutathione peroxidase (GSH-Px) in liver tissues was determined quantitatively. Results Compared with the N group, the liver and spleen index of the M group increased significantly (P<0.001); the content of AST, ALT, and HYP increased significantly (P<0.001); the content of TNF-α and IL-1β increased significantly (P<0.05, P<0.01); the activity of GSH-Px was significantly reduced (P<0.001). Compared with the M group, the liver index of each administration group significantly reduced (P<0.01, P<0.001); the spleen index of QM group significantly reduced (P<0.01); the content of serum AST and ALT of Y group and QH group significantly reduced (P<0.05); the content of HYP of Y group, QH group, QM group significantly reduced (P<0.01, P<0.001), the activity of GSH-Px significantly increased (P<0.01, P<0.001); the TNF-α level of liver tissue of Y group and QM group significantly reduced (P<0.05), the level of IL-1β decreased significantly in all treatment groups (P<0.01, P<0.001); compared with the Y group, the activity of GSH-Px of the QH group was significantly increased (P<0.05). Conclusion Tibetan medicine Qiwei Tiexie Capsule can alleviate CCL4-induced liver fibrosis in rats, which may be related with reduced inflammation, decreased collagen production, and enhanced antioxidant capacity. |
| Key words: Tibetan medicine Qiwei Tiexie Capsule liver fibrosis inflammation antioxidant |
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