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洪慧,张林.绿茶提取物表没食子儿茶素没食子酸酯对腹膜透析患者腹膜纤维化的防治作用及其机制研究[J].湖南中医药大学学报英文版,2022,42(1):49-54.[Click to copy
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| 绿茶提取物表没食子儿茶素没食子酸酯对腹膜透析患者腹膜纤维化的防治作用及其机制研究 |
| 洪慧,张林 |
| (湖南省直中医院肾内科, 湖南 长沙 410000;长沙市第四医院肾内科, 湖南 长沙 410006) |
| 摘要: |
| 目的 探讨绿茶提取物表没食子儿茶素没食子酸酯（epigallocatechin gallate，EGCG）对腹膜透析（peritoneal dialysis，PD）患者腹膜纤维化的防治作用及其机制。方法 培养人腹膜间皮细胞（human peritoneal mesothelial cells，HPMCs），分别以0、12.5、25、50、100 μmol/L的EGCG对HPMCs进行预处理后，采用晚期糖基化终末产物（advanced glycation end products，AGEs）诱导建立上皮-间充质转化（epithelial-mesenchymal transition，EMT）模型，将未做任何处理的细胞作为对照组。采用MTT法分析EGCG对HPMCs增殖的影响；划痕实验法分析EGCG对HPMCs迁移的影响；蛋白质免疫印迹法检测HPMCs上皮细胞分子标志蛋白（Snail、E-cadherin、CK、ZO-1）和间质细胞分子标志蛋白（α-SMA、FSP1）表达水平；上皮跨膜细胞电阻仪检测EGCG对HPMCs跨细胞电阻（transcellular resistance，TER）的影响。结果 与对照组比较，EMT模型组的细胞活力明显增加，Snail、E-cadherin、CK和ZO-1蛋白表达水平降低，α-SMA和FSP1蛋白表达水平升高，TER值增加（P<0.05）；EGCG可以剂量依赖性地减少HPMCs细胞活力，抑制HPMCs细胞迁移，增加Snail、E-cadherin、CK和ZO-1蛋白表达水平，减少α-SMA和FSP1蛋白表达水平，降低TER值（P<0.05）。结论 EGCG可有效抑制HPMCs增殖和迁移，并通过上调上皮细胞分子标志蛋白表达，下调间质细胞分子标志蛋白表达，增加HPMCs通透性，抑制HPMCs的EMT，延缓腹膜纤维化，具有临床应用价值。 |
| 关键词: 表没食子儿茶素没食子酸酯 腹膜透析 人腹膜间皮细胞 上皮-间充质转化 终末期肾脏病 |
| DOI：10.3969/j.issn.1674-070X.2022.01.010 |
| Received:December 13, 2020 |
| 基金项目:湖南省卫生健康委员会课题（B20180473）。 |
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| Effect of epigallocatechin gallate extracted from green tea on peritoneal fibrosis in peritoneal dialysis patients and its mechanism |
| HONG Hui,ZHANG Ling |
| (Department of Nephrology, Hunan Provincial Hospital of Traditional Chinese Medicine, Changsha, Hunan 410000, China;Department of Nephrology, The Fourth Hospital of Changsha, Changsha, Hunan 410006, China) |
| Abstract: |
| Objective To investigate the effect and mechanism of epigallocatechin gallate (EGCG) extracted from green tea on peritoneal fibrosis in peritoneal dialysis (PD) patients. Methods Human peritoneal mesothelial cells (HPMCs) were cultured and pretreated with 0, 12.5, 25, 50, 100 μmol/L EGCG respectively. The epithelial-mesenchymal transition (EMT) model was induced by advanced glycation end products (AGEs). Cells without any treatment were taken as control group. MTT method was used to analyze the effect of EGCG on HPMCs proliferation, scratch test was used to analyze the effect of EGCG on HPMCs migration, Western blot method was used to detect the expression level of HPMCs epithelial molecular marker protein (Snail, E-cadherin, CK, ZO-1) and interstitial molecular marker protein (α-SMA, FSP1), and epithelial transmembrane cell resistance meter was used to detect the effect of EGCG on HPMCs transcellular resistance (TER). Results Compared with the control group, the cell viability of EMT model group was significantly increased, the protein expression levels of Snail, E-cadherin, CK and ZO-1 were decreased, the protein expression levels of α-SMA and FSP1 were increased, and the TER value was increased (P<0.05). EGCG can dose-dependently reduce the viability of HPMCs cells, inhibit the migration of HPMCs cells, increase the protein expression levels of Snail, E-cadherin, CK and ZO-1, decrease the protein expression levels of α-SMA and FSP1, and decrease the TER value (P<0.05). Conclusion EGCG can effectively inhibit the proliferation and migration of HPMCs, increase the permeability of HPMCs, inhibit the EMT of HPMCs, and delay the peritoneal fibrosis by up-regulating the expression of epithelial cell molecular marker protein, down-regulating the expression of interstitial cell molecular marker protein, which has clinical application value. |
| Key words: epigallocatechin gallate peritoneal dialysis human peritoneal mesothelial cells epithelial-mesenchymal transition end-stage renal disease |
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