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杨珊珊,陈红琼,陈瑶,郁洁,罗坚,周薇,彭振,雷晓明.壮骨止痛胶囊对去卵巢大鼠体质量及脂/骨代谢的影响[J].湖南中医药大学学报英文版,2022,42(1):18-24.[Click to copy
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| 壮骨止痛胶囊对去卵巢大鼠体质量及脂/骨代谢的影响 |
| 杨珊珊,陈红琼,陈瑶,郁洁,罗坚,周薇,彭振,雷晓明 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨壮骨止痛胶囊对去卵巢大鼠体质量及脂/骨代谢的影响。方法 选用健康雌性SD大鼠30只，随机分为空白（Blank）组、假手术（Sham）组、模型（OVX）组、壮骨止痛胶囊（ZGZTC）组和雌激素（EV）组，每组6只。OVX组、ZGZTC组以及EV组均摘除双侧卵巢，Sham组仅在卵巢周围切除相应体积的脂肪。ZGZTC组（567 mg/kg）、EV组（0.21 mg/kg）灌胃相应药物，Sham组和OVX组灌胃相应体积生理盐水，Blank组不做处理。每周检测体质量，干预12周后用显微CT检测大鼠右股骨骨密度（bone mineral density，BMD），扫描电镜分析左股骨骨小梁超微结构，HE染色评价胫骨组织微结构的变化，ELISA法测定血清雌激素（estrogen，E2）、脂联素（adiponectin，ADP）、瘦素（leptin，LEP）、骨碱性磷酸酶（bone alkaline phosphatase，BALP）、骨钙素（bone-Gla-protein，BGP）、I型前胶原N端前肽（N-terminal propeptide of type I procollagen，PINP）水平。结果 与Blank组和Sham组比较，OVX组大鼠BMD显著降低（P<0.001），骨小梁孔隙率增加，骨小梁厚度变薄，间距增大，内部骨微结构紊乱，BALP及ADP水平显著升高（P<0.05，P<0.01，P<0.001）。与Blank组比较，OVX组大鼠E2水平显著下降（P<0.05）。与OVX组比较，ZGZTC组大鼠胫骨、股骨骨小梁形态结构改善，骨髓腔内脂肪空泡面积及数目减少（P>0.05），体质量降低（P<0.001），血清E2水平和PINP水平显著升高（P<0.05），BALP水平显著降低（P<0.01）；其血清LEP水平升高，ADP、BGP水平降低，但差异无统计学意义（P>0.05）。结论 壮骨止痛胶囊可能通过调节血清E2、脂肪因子ADP、LEP水平，抑制成脂分化过度，促进成骨分化，减少骨髓脂肪堆积，抑制过度骨代谢，改善骨小梁微结构，防治绝经后骨质疏松症，并维持体质量稳定，减少骨折的发生。 |
| 关键词: 壮骨止痛胶囊 骨质疏松症 脂骨代谢 骨髓脂肪 扫描电镜 骨微结构 |
| DOI：10.3969/j.issn.1674-070X.2022.01.005 |
| Received:March 09, 2021 |
| 基金项目:国家自然科学基金面上项目（81973920）；湖南省教育厅创新平台开放基金项目（20K092）。 |
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| Effect of Zhuanggu Zhitong Capsule on body weight and lipid/bone metabolism in ovariectomized rats |
| YANG Shanshan,CHEN Hongqiong,CHEN Yao,YU Jie,LUO Jian,ZHOU Wei,PENG Zhen,LEI Xiaoming |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the effects of Zhuanggu Zhitong Capsule on body weight and lipid/bone metabolism of ovariectomized rats. Methods 30 healthy female SD rats were randomly divided into Blank group, Sham group, OVX group, ZGZTC group and EV group, with 6 rats in each group. Bilateral ovaries were removed in OVX group, ZGZTC group and EV group, and only the corresponding volume of fat around the ovaries was removed in Sham group. ZGZTC (567 mg/kg) and EV (0.21 mg/kg) groups were intragastricted with corresponding drugs, Sham group and OVX group were intragastricted with corresponding volume of normal saline, Blank group was not treated. The body weight was measured every week. After 12 weeks of intervention, the bone mineral density (BMD) of the rat's right femur was measured by micro-CT, the ultrastructure of the trabecular bone of the left femur was analyzed by scanning electron microscope (SEM), the changes of tibial tissue microstructure were evaluated by HE staining, ELISA method was used to determine serum estrogen (E2), adiponectin (ADP), leptin (LEP), bone alkaline phosphatase (BALP), bone-Gla-protein (BGP), N-terminal propeptide of type I procollagen (PINP) levels. Results Compared with Blank group and Sham group, BMD of rats from OVX group was significantly decreased (P<0.001), and the porosity of bone trabecular was increased, the thickness was thinner and spacing of bone trabecular increased, the internal bone microstructure was disturbed, and the levels of BALP and ADP were significantly increased (P<0.05, P<0.01, P<0.001). Compared with Blank group, E2 level of rats from OVX group was significantly decreased (P<0.05). Compared with OVX group, the morphology and structure of tibia and femur trabecular bone of rats from ZGZTC group were improved, and the area and number of fat cavitation in bone marrow cavity were decreased (P>0.05), body weight was decreased (P<0.001), serum E2 and PINP levels were significantly increased (P<0.05), and BALP level was significantly decreased (P<0.01), serum LEP level was increased and ADP and BGP levels were decreased, but the differences were not statistically significant (P>0.05). Conclusion Zhuanggu Zhitong Capsule may inhibit excessive adipogenic differentiation, promote osteogenic differentiation, reduce bone marrow fat accumulation, inhibit excessive bone metabolism, improve bone trabecular microstructure, prevent and cure postmenopausal osteoporosis, maintain stable body mass and reduce the occurrence of fractures by regulating serum E2, adipogenic factor ADP and LEP levels. |
| Key words: Zhuanggu Zhitong Capsule osteoporosis lipid bone metabolism bone marrow fat scanning electron microscope bone microstructure |
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