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王珊,刘红梅,刘兵,谭文波,童巧珍,刘湘丹.UPLC-MS/MS法同时测定杞菊地黄胶囊中7种有效成分[J].湖南中医药大学学报英文版,2021,41(12):1876-1880.[Click to copy
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| UPLC-MS/MS法同时测定杞菊地黄胶囊中7种有效成分 |
| 王珊,刘红梅,刘兵,谭文波,童巧珍,刘湘丹 |
| (湖南中医药大学第二附属医院, 湖南 长沙 410005;湖南中医药大学药学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 建立超高效液相色谱-串联质谱（UPLC-MS/MS）同时测定杞菊地黄胶囊中莫诺苷、绿原酸、马钱苷、木犀草苷、丹皮酚、23-乙酰泽泻醇C、23-乙酰泽泻醇B的含量。方法 采用ACQUITY UPLC® HSS T3（100 mm×2.1 mm，1.8 μm）为色谱柱，乙腈-0.1%甲酸水溶液为流动相，梯度洗脱，流速为0.3 mL·min-1；采用电喷雾电离源（ESI）、多反应离子监测（MRM）模式对7种有效成分进行测定。结果 每粒杞菊地黄胶囊样品中莫诺苷含量为0.28~0.35 mg，绿原酸含量为0.08~0.11 mg，马钱苷含量为0.30~0.48 mg，木犀草苷含量为0.21~0.48 mg，丹皮酚含量为0.62~0.76 mg，23-乙酰泽泻醇C含量为0.04~0.08 mg，23-乙酰泽泻醇B含量为0.15~0.32 mg。7种有效成分相关系数为0.999 0~1.000 0；平均回收率（n=6）为97.72%~99.12%，RSD为0.61%~2.01%。结论 该方法专属性强、灵敏高、重复性好，适用于杞菊地黄胶囊中莫诺苷、绿原酸、马钱苷、木犀草苷、丹皮酚、23-乙酰泽泻醇C和23-乙酰泽泻醇B 7种有效成分的同时测定。 |
| 关键词: 杞菊地黄胶囊 超高效液相色谱-串联质谱 莫诺苷 绿原酸 马钱苷 木犀草苷 丹皮酚 23-乙酰泽泻醇C 23-乙酰泽泻醇B |
| DOI：10.3969/j.issn.1674-070X.2021.12.011 |
| Received:July 27, 2021 |
| 基金项目:财政部和农业农村部：国家现代农业产业技术体系资助项目（CARS-21）；湖南省现代农业产业技术体系建设专项。 |
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| Simultaneous Determination of Seven Effective Components in Qiju Dihuang Capsule by UPLC-MS/MS |
| WANG Shan,LIU Hongmei,LIU Bing,TAN Wenbo,TONG Qiaozhen,LIU Xiangdan |
| (The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To establish an UPLC-MS/MS method for simultaneous determination of seven effective components (morroniside, chlorogenic acid, loganin, luteolin-7-O-glucoside, paeonol, 23-acetate alisol C, 23-acetate alisol B) in Qiju Dihuang Capsule. Methods The ACQUITY UPLC® HSS T3 (100 mm×2.1 mm, 1.8 μm) column was adopted with mobile phase consisted of acetonitrile-0.1%-formic acid with gradient elution at the flow rate of 0.3 mL·min-1. Electrospray ionization (ESI) and multiple reactive ion monitoring (MRM) modes were used to determine the seven active components. Results The contents of morroniside, chlorogenic acid, loganin, luteolin-7-O-glucoside, paeonol, 23-acetate alisol C, 23-acetate alisol B in the samples per capsule were 0.28~0.35, 0.08~0.11, 0.30~0.48, 0.21~0.48, 0.62~0.76, 0.04~0.08, 0.15~0.32 mg. Correlation coefficient values of the 7 active components were among 0.999 0~1.000 0; the average recovery rates were among 97.72%~99.12% (n=6), and the RSD were among 0.61%~2.01%. Conclusion The method established is specific, sensitive and reproducible, which is suitable for the determination of seven effective components (morroniside, chlorogenic acid, loganin, luteolin-7-O-glucoside, paeonol, 23-acetate alisol C, 23-acetate alisol B) in Qiju Dihuang Capsule. |
| Key words: Qiju Dihuang Capsule UPLC-MS/MS morroniside chlorogenic acid loganin luteolin-7-O-glucoside paeonol 23-acetate alisol C 23-acetate alisol B |
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