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邓楚欣,于征淼,林培政,吴智兵.半夏生物总碱对健康小鼠运动平衡、自主活动及空间工作记忆能力的影响[J].湖南中医药大学学报英文版,2021,41(11):1676-1682.[Click to copy
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| 半夏生物总碱对健康小鼠运动平衡、自主活动及空间工作记忆能力的影响 |
| 邓楚欣,于征淼,林培政,吴智兵 |
| (广州中医药大学第一附属医院, 广东 广州 510405;广州中医药大学, 广东 广州 510000) |
| 摘要: |
| 目的 使用与运动平衡、自主活动及空间工作记忆功能相关的行为学测试，观察半夏生物总碱（pinellia total alkaloids，PTA）对健康雄性小鼠神经系统的运动和认知功能的抑制作用，从而评价PTA的神经毒性。方法 限量试验取30例4周龄雄性KM小鼠，随机分为PTA组（2 g/kg）和空白组，每组15只，灌胃1次，在灌胃前（0 h）和灌胃后8 h、7 d、14 d进行转棒测试及Y臂迷宫测试。28 d重复剂量试验取60例4周龄雄性KM小鼠，随机分为PTA低、中、高剂量组（0.08、0.28、1 g/kg）及空白组，每组15只，连续灌胃28 d；在首剂灌胃前（0 h）和首剂灌胃后14 d、28 d、35 d进行转棒测试及Y臂迷宫测试。取脑和脊髓组织行HE染色病检，观察额叶、尾状核、海马、黑质、脑桥、小脑及脊髓前角的组织形态学改变。结果 限量测试：与空白组比，PTA组体质量在7 d和14 d较高（P<0.05），Y臂迷宫的自发路线转换行为比（SAB%）在8 h升高（P<0.05）。28 d重复剂量试验：与自身0 h比，PTA高剂量组的SAB%在28 d升高（P<0.05）。病检未见明显异常。结论 在本研究的给药方案及评价指标下，PTA未对雄性小鼠产生显著的神经毒性，个别时间点出现的行为学水平提高的意义尚不明确。 |
| 关键词: 半夏生物总碱 神经毒性 运动平衡 自主活动 空间工作记忆 病理 小鼠 |
| DOI：10.3969/j.issn.1674-070X.2021.11.006 |
| Received:June 07, 2021 |
| 基金项目:广东省中医药局项目（211020030624）。 |
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| Effect of Pinellia Total Alkaloids on Motor Coordination, Locomotion, and Spatial Working Memory on Healthy Mice |
| DENG Chuxin,YU Zhengmiao,LIN Peizheng,WU Zhibing |
| (The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, China;Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, China) |
| Abstract: |
| Objective Neurotoxicity evaluation concerning motor and cognitive inhibition of pinellia total alkaloids (PTA) on healthy mice was conducted by using behavioral tests related to motor coordination, locomotion, and spatial working memory. Methods In the limit test, 30 male KM mice (4 weeks old) were divided into a PTA group (2 g/kg) and a control group, with 15 mice in each group, and were intragastrically dosed once. The rotarod test (RT) and the Y-maze test (YMT) were examined before dosing (0 hour) and at 8 hours, 7 and 14 days after dosing. In the repeated 28-day dosing test, 60 male KM mice (4 weeks old) were assigned to the PTA low, medium, and high dose (0.08, 0.28, 1 g/kg) groups and the control group, with 15 mice in each group. Mice were intragastrically dosed once daily for 28 days. The RT and YMT were examined before the first dose (0 hour) and at 14, 28 and 35 days after the first dose. HE staining was conducted on sections of the frontal motor cortex, caudate putamen, hippocampal formation, substantia nigra, pons, cerebellum cortex, and ventral horns of the spinal cord. Results In the limit test, compared with the control group, the PTA group showed higher body weight at 7 and 14 days (P<0.05), and higher spontaneous alternation behavior (SAB%) at 8 hours (P<0.05). In the repeated 28-day dosing test, compared with 0 hour, the PTA high dose group showed higher SAB% at 28 days (P<0.05). No significant histopathological changes were observed. Conclusion With the dosing regimens and indicators used in this study, PTA did not induce significant neurotoxicity in healthy male mice, the significance of increased behavioral responses detected in certain time points remained undefined. |
| Key words: pinellia total alkaloids neurotoxicity motor coordination locomotion spatial working memory histopathology mice |
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