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彭礼真,席建元,祁林.银屑平丸对银屑病样小鼠模型皮损中Notch1和Jagged1的影响[J].湖南中医药大学学报英文版,2021,41(10):1546-1550.[Click to copy
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| 银屑平丸对银屑病样小鼠模型皮损中Notch1和Jagged1的影响 |
| 彭礼真,席建元,祁林 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 探讨银屑平丸对银屑病样小鼠模型Notch1和Jagged1表达水平的影响。方法 将48只小鼠随机分为空白组、模型组、阿维A胶囊组、银屑平丸高、中、低剂量组，每组8只。各组小鼠背部脱毛后，每日外用咪喹莫特乳膏造模形成银屑病样小鼠，干预方式是同时予以相应药物给每组小鼠灌胃。观察并记录小鼠背部皮肤，予银屑病皮损面积与严重度指数（PASI）评分；运用形态观察、HE染色病理分析皮损组织形态学变化；免疫组化、反转录聚合酶链反应方法来检测小鼠皮肤组织Notch信号通路中Notch1、Jagged1的表达。结果 与空白组相比，模型组呈现明显的银屑病病理变化，在光镜下可表现为不同程度的角化不全，乳头上棘层变薄，乳头水肿，血管扩张充血；而银屑平丸高、中、低剂量组及阿维A胶囊组变化均较少。与空白组相比，模型组、银屑平丸高、中、低剂量组及阿维A胶囊组造模后PASI评分均升高（P<0.05）；与模型组相比，银屑平丸高、中、低剂量组及阿维A胶囊组造模后PASI评分均降低（P<0.05），其中银屑平丸高剂量组PASI评分降低最明显，其次为阿维A胶囊组（P<0.05）。与空白组相比，模型组Notch1、Jagged1表达量均增加（P<0.05）；与模型组相比，银屑平丸高、中、低剂量组及阿维A胶囊组Notch1、Jagged1表达量均降低（P<0.05）。与空白组相比，模型组Notch1、Jagged1 mRNA表达量均增加（P<0.05）；与模型组相比，银屑平丸高、中、低剂量组及阿维A胶囊组Notch1、Jagged1 mRNA表达量均降低（P<0.05）。结论 银屑平丸能够降低银屑病样小鼠模型的PASI评分，使银屑病的皮损能够改善；银屑病可能与Notch1/Jagged1信号通路相关联；银屑平丸治疗银屑病可能是通过降低Notch1、Jagged1的方式来表达。 |
| 关键词: 银屑平丸 银屑病 Notch1 Jagged1 阿维A胶囊 |
| DOI：10.3969/j.issn.1674-070X.2021.10.013 |
| Received:March 27, 2021 |
| 基金项目:湖南省科技创新项目（S2017SFYLJS0115）。 |
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| Effects of Yinxieping Pills on the Notch1 and Jagged1 in Skin Lesion of Psoriasis-like Mice Models |
| PENG Lizhen,XI Jianyuan,QI Lin |
| (The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To investigate the effect of Yinxieping Pill on the expression levels of Notch1 and Jagged1 in murine psoriasis model. Methods 48 mice were randomly divided into blank group, model group, acitretin capsule group, Yinxieping Pill high-dose, medium-dose and low-dose groups, with 8 mice in each group. After the back hair removal of mice, imiquimod cream was used daily to induce psoriasis-like mouse model. The intervention method was to give corresponding drugs to each group by gavage at the same time. The skin on the back of mice was observed and recorded, and the psoriasis area and severity index (PASI) was scored. Morphological observation and HE staining were used to analyze the histological changes of skin lesions. Immunohistochemistry, reverse transcription polymerase chain reaction were used to detect the expression of Notch1 and Jagged1 of Notch signaling pathway in mouse skin. Results Compared with the blank group, the model group showed obvious pathological changes of psoriasis, which can be manifested as different degrees of incomplete keratosis under light microscope, thinning of the spine layer of the nipple, nipple edema, vasodilation and hyperemia. However, there were fewer changes in Yinxieping Pill high-dose, medium-dose and low-dose groups and acitretin capsule group. Compared with the blank group, the PASI scores of the skin lesions in the model group, Yinxieping Pill high-dose, medium-dose and low-dose groups and acitretin capsule group were significantly increased (P<0.05); compared with the model group, the PASI scores of the skin lesions in the Yinxieping Pill high-dose, medium-dose and low-dose groups and acitretin capsule group decreased after modeling (P<0.05), among them, the PASI score of the Yinxieping Pill high-dose group had the most significant decrease, followed by the acitretin capsule group (P<0.05). Compared with the blank group, the expression levels of Notch1 and Jagged1 in the model group were significantly increased (P<0.05); compared with the model group, the expression levels of Notch1 and Jagged1 in the Yinxieping Pill high-dose, medium-dose and low-dose groups and acitretin capsule group were significantly decreased (P<0.05). Compared with the blank group, the expression levels of Notch1 and Jagged1 mRNA in the model group were significantly increased (P<0.05); compared with the model group, the expression levels of Notch1 and Jagged1 mRNA in the Yinxieping Pill high-dose, medium-dose and low-dose groups and acitretin capsule group were significantly decreased (P<0.05). Conclusion Yinxieping Pill can decrease PASI score of psoriasis-like mouse model, and has the effect of improving psoriatic lesions. The occurrence of psoriasis may be associated with Notch1/Jagged1 signaling pathways. Yinxieping Pill can reduce the expression of Notch1 and Jagged1, which has a certain significance for the treatment of psoriasis. |
| Key words: Yinxieping Pill psoriasis Notch1 Jagged1 acitretin capsule |
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