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卢园,陈海南,杨舟,薛惠天,王兰兰,孙梦龙,彭亮.小鱼际滚法对兔骨骼肌急性钝搓伤组织机化期GDF-8/Smad2通路的影响[J].湖南中医药大学学报英文版,2021,41(9):1345-1349.[Click to copy
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| 小鱼际滚法对兔骨骼肌急性钝搓伤组织机化期GDF-8/Smad2通路的影响 |
| 卢园,陈海南,杨舟,薛惠天,王兰兰,孙梦龙,彭亮 |
| (湖南中医药大学针灸推拿学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察小鱼际滚法干预新西兰白兔骨骼肌急性钝挫伤组织机化期的生长/分化因子8（growth differentiation factor 8，GDF-8）表达、受体活化型通路限制性蛋白Smad2、Smad3的表达水平以及Smad2的磷酸化水平（p-Smad2），探究小鱼际滚法干预骨骼肌急性钝挫伤组织机化期的可能作用机制。方法 采用随机数字表法将18只兔分为空白组、模型组、滚法组，每组6只。采用自制重力锤打击装置制备新西兰白兔骨骼肌急性钝挫伤模型，于造模后第5天进行干预，空白组和模型组进行捆绑，滚法组由经过专门培训的专员进行小鱼际滚法干预，皆为上午下午各1次，5 min/次，共3 d。干预结束后，观察苏木精-伊红（hematoxylin-eosin，HE）染色组织形态学变化，采用蛋白质印迹法（Western blot，WB）检测GDF-8、p-Smad2、Smad2/3蛋白相对表达量。结果 HE染色结果显示，模型组的股四头肌肌束间不规则结缔样组织明显增多，肌纤维排列紊乱，数量减少、变小，滚法组存在少量结缔组织，肌束排列紧密，肌纤维排列较为整齐，大小近似。WB检测显示，与空白组比较，模型组和滚法组的GDF-8表达都升高（P<0.01），滚法组表达水平低于模型组（P<0.01）；Smad2/3在各组的蛋白表达一致（P>0.05）；与空白组比较，模型组的p-Smad2蛋白表达、p-Smad2/Smad2/3的灰度比值都升高（P<0.01），滚法组均显著低于模型组（P<0.01）。结论 小鱼际滚法在兔急性钝挫伤组织机化期可能通过抑制GDF-8/Smad2信号通路达到延缓组织纤维化、促进肌纤维生长以及加强骨骼肌修复的目的。 |
| 关键词: 骨骼肌损伤 钝挫伤 推拿 小鱼际滚法 GDF-8 Smad2 |
| DOI：10.3969/j.issn.1674-070X.2021.09.006 |
| Received:April 03, 2021 |
| 基金项目:湖南省教育厅优秀青年项目（19B438）；湖南省学位与研究生教育改革研究项目（217）；湖南省普通高校教学改革项目（385）。 |
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| The Effects of Hypothenar Rolling Method on GDF-8/Smad2 in Tissue Mechanization Stage of Acute Blunt Rubbing Injury in Skeletal Muscle of Rabbit |
| LU Yuan,CHEN Hainan,YANG Zhou,XUE Huitian,WANG Lanlan,SUN Menglong,PENG Liang |
| (College of Acupuncture and Massage, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the effects of hypothenar rolling manipulation on the expression of growth differentiation factor 8 (GDF-8), receptor activated pathway limiting protein Smad2, Smad3, phosphorylation of Smad2 (p-Smad2) in skeletal muscle of New Zealand white rabbits during the tissue mechanization stage of acute blunt muscle contusion, and to explore the possible mechanism of hypothenar rolling in the intervention of skeletal muscle acute blunt trauma tissue during the tissue mechanization stage. Methods 18 rabbits randomly divided into blank group, model group, manipulation group according to random number table method, with 6 rabbits in each group. All rabbits except control group induced skeletal muscle acute blunt contusion model of New Zealand white rabbits by manufacture of self-made gravity hammer striking device. Different intervention methods were performed after 5th day. Blank group and model group were bundled, manipulation group was intervened by the specially trained specialists in the hypothenar rolling method, once in the morning and afternoon, 5 minutes/time for 3 days. After the intervention, hematoxylin-eosin (HE) staining was observed, and the relative protein expression levels of GDF-8, p-Smad2 and Smad2/3 were detected by Western blot (WB). Rseults HE staining results showed that the irregular connective tissue between the muscle bundles of the quadriceps femoris in the model group was significantly increased, and the muscle fibers were disordered, and the number was reduced or decreased. In the manipulation group, there was a small amount of connective tissue, the muscle bundles were closely arranged, and the muscle fibers were orderly and similar in size. WB assay showed that compared with blank group, GDF-8 expression was increased in both model group and manipulation group (P<0.01), while the expression level in manipulation group was lower than that in model group (P<0.01). The protein expression of Smad2/3 was consistent in all groups (P>0.05). Compared with blank group, p-Smad2 protein expression and p-Smad2/Smad2/3 gray ratio in model group were increased (P<0.01), while manipulation group was significantly lower than model group (P<0.01). Conclusion The hypothenar rolling method may delay tissue fibrosis, promote muscle fiber growth and enhance skeletal muscle repair by inhibiting GDF-8/Smad2 signaling pathway during the tissue mechanization stage of acute blunt contusion in rabbits. |
| Key words: muscle injury blunt injury Tuina hypothenar rolling manipulation GDF-8 Smad2 |
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