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肖慧,彭林娟,熊武,白雪,谭梅鑫,李叶兰,杨莹,朱晨鸿,邹晓玲.阿魏酸对间充质干细胞增殖、分泌干细胞因子和成管分化的影响[J].湖南中医药大学学报英文版,2021,41(8):1160-1165.[Click to copy
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| 阿魏酸对间充质干细胞增殖、分泌干细胞因子和成管分化的影响 |
| 肖慧,彭林娟,熊武,白雪,谭梅鑫,李叶兰,杨莹,朱晨鸿,邹晓玲 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 探讨阿魏酸（ferulic acid，FA）对间充质干细胞（mesenchymal stem cells，MSCs）增殖、分泌干细胞因子（stem cell factor，SCF）和定向内皮细胞成管分化的影响。方法 取足月健康新生儿脐带血10 mL，采用密度梯度离心法得到单个细胞，并鉴定人脐带血间充质干细胞（human umbilical cord blood mesenchymal stem cells，hUCBMSCs）分化能力。将hUCBMSCs分别用不同浓度梯度的FA （0、1、2、4、8、16 mg/L）干预以确定FA促hUCBMSCs增殖的最佳浓度。取hUCBMSCs随机分为实验组与对照组，实验组用最佳浓度FA干预，对照组用等体积PBS液处理。分别采用CCK-8细胞增殖试验、Matrigel体外成管试验及ELISA法检测两组hUCBMSCs增殖、成管及分泌SCF的能力；采用免疫荧光法检测hUCBMSCs向内皮细胞分化后CD31、vWF的表达情况。结果 （1） hUCBMSCs能成功诱导分化为骨细胞、软骨细胞、脂肪细胞；（2） FA促进hUCBMSCs增殖的最佳浓度为2 mg/L；（3）与对照组相比，实验组hUCBMSCs增殖、成管及分泌SCF能力显著增强，且FA诱导成管分化后CD31和vWF的表达明显增多（P<0.05）。结论 FA能促进hUCBMSCs增殖及分泌SCF，同时能诱导hUCBMSCs成管分化，进一步证实FA具有促进血管新生的潜能。 |
| 关键词: 阿魏酸 间充质干细胞 细胞增殖 干细胞因子 成管分化 血管新生 |
| DOI：10.3969/j.issn.1674-070X.2021.08.005 |
| Received:January 05, 2021 |
| 基金项目:国家自然科学基金青年基金项目（81904217）；湖南省卫生健康委一般资助课题项目（20201388）。 |
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| Effects of Ferulic Acid on the Proliferation, Stem Cell Factor Secretion and Tube Differentiation of Mesenchymal Stem Cells |
| XIAO Hui,PENG Linjuan,XIONG Wu,BAI Xue,TAN Meixin,LI Yelan,YANG Ying,ZHU Chenhong,ZOU Xiaoling |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To study the effects of ferulic acid (FA) on the proliferation, the secretion of stem cell factor (SCF) and the differentiation of targeted endothelial cells by mesenchymal stem cells (MSCs). Methods Single nuclear cells were isolated by density gradient centrifugation from cord blood of full-term healthy newborns, and the differentiation ability of human umbilical cord blood mesenchymal stem cells (hUCBMSCs) were identified. hUCBMSCs were intervened with FA with different concentration gradients (0, 1, 2, 4, 8, 16 mg/L) to determine the optimal concentration of FA to promote the proliferation of hUCBMSCs. hUCBMSCs were randomly divided into the experimental group and the control group. The experimental group was intervened with the best concentration of FA, the control group was treated with PBS solution of equal volume. CCK-8 cell proliferation test, Matrigel in vitro tube test and ELISA method were used to detect the proliferation, tube formation and SCF secretion ability of the two groups of hUCBMSCs; immunofluorescence method was used to detect the expression of CD31 and vWF after hUCBMSCs differentiated into endothelial cells. Results (1) hUCBMSCs can successfully induce differentiation into bone cells, chondrocytes and adipocytes. (2) The optimal concentration of FA to promote the proliferation of hUCBMSCs is 2 mg/L. (3) Compared with the control group, the ability of hUCBMSCs in the experimental group to proliferate, form tubes and secrete SCF was significantly enhanced, and the expression of CD31 and vWF increased significantly after FA induced tube differentiation (P<0.05). Conclusion FA can promote the proliferation, the secretion of SCF from hUCBMSCs, and induce the differentiation of hUCBMSCs into tubes, further confirming that FA has the potential to promote angiogenesis. |
| Key words: ferulic acid mesenchymal stem cell cell proliferation stem cell factor tube differentiation angiogenesis |
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