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黄新宇,陈丽,周忠志,胡晋婷,王霄霜,杨丁丁,王巍,叶永鑫,袁忠行,丁雅容,禚昭钦,刘平安.仙藕乳蒲方双向调节整合素β1、α-SMA对小鼠皮肤移植创面愈合的影响[J].湖南中医药大学学报英文版,2021,41(7):1017-1021.[Click to copy
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| 仙藕乳蒲方双向调节整合素β1、α-SMA对小鼠皮肤移植创面愈合的影响 |
| 黄新宇,陈丽,周忠志,胡晋婷,王霄霜,杨丁丁,王巍,叶永鑫,袁忠行,丁雅容,禚昭钦,刘平安 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学中西医结合学科, 湖南 长沙 410208) |
| 摘要: |
| 目的 从调节整合素β1、α-SMA探究仙藕乳蒲方促小鼠皮肤移植创面愈合的机制。方法 选用40只雌性C57BL/6小鼠，制作背部直径为1.2 cm圆形全层皮肤缺损模型，后进行自体原位皮肤移植，随机分为对照组和实验组，实验组给予仙藕乳蒲方粉末敷于皮肤移植区，对照组不予处理。免疫组织化学染色法比较组织整合素β1、α-SMA的含量；Masson染色比较两组皮肤移植区胶原纤维分布。结果 （1）免疫组化统计结果示：皮肤移植14 d，实验组整合素β1、α-SMA表达明显高于对照组（P<0.05）；皮肤移植21 d，实验组整合素β1、α-SMA表达则低于对照组（P<0.05）。（2）Masson染色显示：与对照组相比，皮肤移植14 d，实验组真皮层胶原纤维丰富，排列致密有序；皮肤移植21 d，实验组真皮层胶原纤维更少沉积，且排列疏松规则。结论 仙藕乳蒲方在皮肤移植创面愈合早期，可提高整合素β1、α-SMA的表达，加强胶原纤维的合成分泌并加快创面收缩；在创面愈合晚期，降低整合素β1、α-SMA的表达，减少胶原纤维的合成分泌并防止创面过度收缩。仙藕乳蒲方可能通过双向调节整合素β1、α-SMA的表达，发挥促进皮肤移植创面愈合及降低纤维化的作用，为仙藕乳蒲方运用于临床皮肤移植创面愈合提供依据。 |
| 关键词: 仙藕乳蒲方 皮肤移植 创面愈合 整合素β1 α-SMA |
| DOI：10.3969/j.issn.1674-070X.2021.07.008 |
| Received:March 28, 2021 |
| 基金项目:国家自然科学基金项目（81973862）；湖南省教育厅科研项目（18A207）；湖南省长沙市科技局项目（kq1801047）；湖南省大学生创新创业训练计划项目（S201910541006，S201910541016）。 |
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| Effect of Xian'ou Rupu Recipe on the Wound Healing of Mice Skin Transplantation by Regulating Integrin β1 and α-SMA Bidirectionally |
| HUANG Xinyu,CHEN Li,ZHOU Zhongzhi,HU Jinting,WANG Xiaoshuang,YANG Dingding,WANG Wei,YE Yongxin,YUAN Zhonghang,DING Yarong,ZHUO Zhaoqin,LIU Ping'an |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Discipline of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the mechanism of the Xian'ou Rupu Recipe promoting the healing of mouse skin graft wounds by regulating integrin β1 and α-SMA. Methods 40 female C57BL/6 mice were used to make a circular full-thickness skin defect model with a diameter of 1.2 cm on the back, autologous orthotopic skin graft were performed and divided into control group and experimental group. In the experimental group, the Xian'ou Rupu Recipe powder was applied to the skin grafting area, and the control group was not treated. Immunohistochemical staining was used to compare the content of integrin β1 and α-SMA; Masson staining was used to compare the distribution of collagen fibers in the skin graft area between the two groups. Results (1) The statistical results of immunohistochemistry showed that the expression levels of integrin β1 and α-SMA in the experimental group were significantly higher than that in the control group at 14 days after skin transplantation (P<0.05); at 21 days after the skin transplantation, the expression levels of integrin β1 and α-SMA in the experimental group were lower than that in the control group (P<0.05). (2) Masson staining showed that compared with the control group, 14 days after skin transplantation, the experimental group had rich collagen fibers in the dermis layer, and the arrangement was dense and orderly; 21 days after skin transplantation, the experimental group had less deposition of collagen fibers in the dermis layer, and the arrangement was loose and regular. Conclusion The Xian'ou Rupu Recipe can increase the expression of integrin β1, α-SMA, strengthen the synthesis and secretion of collagen fibers and accelerate the contraction of wounds in the early stage of skin graft wound healing; in the late stage of wound healing, reduce the expression of integrin β1, α-SMA, reduce synthesis and secretion of collagen fibers and prevent excessive contraction of the wound surface. It was confirmed that the Xian'ou Rupu Recipe may play a role in promoting skin graft wound healing and reducing fibrosis by bidirectionally regulating the expression of integrin β1 and α-SMA, providing a basis for the application of the Xian'ou Rupu Recipe in clinical skin graft wound healing. |
| Key words: Xian'ou Rupu Recipe skin transplantation wound healing integrin β1 α-SMA |
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