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周宵,魏彬彬,唐标.基于生物信息学从体质转化角度探讨湿性体质的关键基因与通路[J].湖南中医药大学学报英文版,2021,41(6):881-886.[Click to copy
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| 基于生物信息学从体质转化角度探讨湿性体质的关键基因与通路 |
| 周宵,魏彬彬,唐标 |
| (湖南中医药大学医学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 通过生物信息数据挖掘，从体质转化角度进行差异分析，探讨湿性体质关键的生物学机制。方法 通过高通量基因表达数据库（gene expression omnibus data base，GEO）下载芯片"GSE140769"，将平和体质转变为湿性体质的前后血清高通量蛋白测序的结果进行筛选，获得12个显著差异蛋白；将湿性体质转变为平和体质前后的血清高通量蛋白测序的结果进行差异分析，得到14个显著差异蛋白，将其与平和体质转变为湿性体质的12个显著差异蛋白进行交叉比对，得到4个介导平和体质与湿性体质的关键差异蛋白。利用R语言、KOBAS 3.0、DAVID 6.8等生物信息分析工具对其进行注释分析。结果 平和体质转变为湿性体质的显著差异蛋白GO分析显示，主要参与了细胞质应激颗粒等细胞组成、神经体细胞调节等生物过程、受体配体活性等分子功能；通路分析显示，主要与淋巴细胞和非淋巴细胞介导的免疫相互作用以及MSP/RON信号通路有关；疾病分析主要与溃疡性结肠炎、慢性阻塞性肺疾病等相关。平和体质与湿性体质的4个关键差异蛋白（MSP、DPPII、Siglec-7、TREML1），分别在平和体质中下调，湿性体质中上调，进一步分析发现该4个蛋白主要介导了细胞的免疫应答与炎症反应，以及MSP/RON信号通路。结论 MSP、DPPII、Siglec-7、TREML1介导的免疫应答与炎症反应，以及MSP/RON信号通路可能是介导平和体质与湿性体质差异关键的生物学基础。 |
| 关键词: 湿性体质 差异表达蛋白 关键蛋白 平和体质 生物学基础 |
| DOI：10.3969/j.issn.1674-070X.2021.06.011 |
| Received:July 29, 2020 |
| 基金项目:湖南省教育厅科学研究项目优秀青年项目（19B436）；湖南省大学生创新创业训练计划项目（X201910541028）。 |
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| The Key Genes and Pathways of Dampness Constitution from the Perspective of Constitution Transformation Based on Bioinformatics |
| ZHOU Xiao,WEI Bingbing,TANG Biao |
| (Medical School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the key biological mechanism of dampness constitution (DC) from the perspective of physique conversion by mining biological information data and analyzing the difference. Methods Chip “GSE140769” was downloaded through gene expression omnibus data base (GEO). The results of high-throughput protein sequencing before and after the transformation from balanced constitution (BC) to DC were screened, and 12 significant difference proteins were obtained; through the difference analysis of serum high-throughput protein sequencing results before and after the transformation from DC to BC, 14 significant difference proteins were obtained, which were cross compared with 12 significant difference proteins before and after the transformation from BC to DC, and 4 key difference proteins were obtained. R language, KOBAS 3.0, DAVID 6.8 and other bioinformatics analysis tools were used for annotation analysis. Results GO analysis of the significant difference proteins in the transformation from BC to DC showed that it was mainly involved in cell composition such as cytoplasmic stress particles, biological processes such as neural somatic cell regulation, and molecular functions such as receptor ligand activity; KEGG pathway analysis showed that it is mainly related to immune regulatory interactions between a lymphoid and a non-lymphoid cell and MSP/RON signaling pathway; disease analysis is mainly related to ulcerative colitis and chronic obstructive pulmonary disease. The four key differential proteins (MSP, DPPII, Siglec-7, TREML1) between BC and DC were down-regulated in BC and up-regulated in DC, and further analysis founded that the four proteins mainly mediate the cellular immune response and inflammation, and MSP/RON signaling pathway. Conclusion MSP, DPPII, Siglec-7, TREML1 mediated immune response and inflammation, and MSP/RON signaling pathway may be the key biological basis for the difference between BC and DC. |
| Key words: differentially expressed proteins key proteins constitution biological basis |
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