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林也,戴宗顺,张婷,张逢,胡胜涛,张二兵,宋厚盼,陈聪,蔡雄,廖菁.基于“以方测证”的类风湿关节炎风寒湿痹证动物模型的构建研究[J].湖南中医药大学学报英文版,2021,41(5):668-672.[Click to copy
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| 基于“以方测证”的类风湿关节炎风寒湿痹证动物模型的构建研究 |
| 林也,戴宗顺,张婷,张逢,胡胜涛,张二兵,宋厚盼,陈聪,蔡雄,廖菁 |
| (湖南中医药大学中药粉体与创新药物省部共建国家重点实验室(培育基地), 湖南长沙 410208;湖南中医药大学中医方证转化医学研究湖南省重点实验室, 湖南长沙 410208) |
| 摘要: |
| 目的 基于以方测证理论建立与类风湿关节炎（RA）病变特征、发病机制相符的中医“痹证”动物模型。方法 将40只雄性SD大鼠随机分为空白组、风寒湿痹证模型组、风寒湿痹证模型+乌头汤组（乌头汤组）、风寒湿痹证模型+四妙丸组（四妙丸组），每组10只大鼠。空白组大鼠无任何干预措施，余各组大鼠均先予以风寒湿环境刺激14 d，再诱导建立佐剂性关节炎（AIA）大鼠模型，同时乌头汤组与四妙丸组分别给予乌头汤和四妙丸灌胃治疗，直至实验结束。记录各组大鼠发病时间和发病率；动态检测大鼠足肿胀体积和评价关节炎指数积分；HE染色观察大鼠踝关节病理变化；CT检测大鼠骨关节破环情况。结果 与风寒湿痹证模型组比较，乌头汤明显延迟大鼠发病时间并降低其发病率，显著减轻大鼠关节肿胀率及关节炎指数评分，明显抑制滑膜组织增生及骨破坏程度（P<0.01），而四妙丸组大鼠病变程度与风寒湿痹证模型组无明显差别（P>0.05）。结论 基于以方测证思路，成功复制了与RA病变特征和发病机制相符的RA风寒湿痹病证结合大鼠模型，为系统深入研究治疗RA方证对应分子机制奠定了基础。 |
| 关键词: 类风湿关节炎 风湿痹证 方证对应 佐剂性关节炎 乌头汤 四妙丸 |
| DOI：10.3969/j.issn.1674-070X.2021.05.003 |
| Received:December 22, 2020 |
| 基金项目:国家自然科学基金项目（81703920）；湖南省“芙蓉学者奖励计划”项目（湘教通[2020]58号）；湖南省自然科学基金项目（2018JJ2293，2019JJ40225）；湖南省121创新人才培养工程项目（湘人社函[2019]192号）；湖南省“刘良院士专家工作站”项目（湘科协通[2020]34号）；湖南省高校创新平台开放基金项目（17K069）；湖南省中医药科研计划项目（201909）；湖南中医药大学中医学国内一流建设学科项目（校行科字[2018]3号）。 |
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| Construction of Animal Model of Rheumatoid Arthritis with Wind Cold Dampness Arthralgia Syndrome Based on the Theory of “Testing Syndrome by Formula” |
| LIN Ye,DAI Zongshun,ZHANG Ting,ZHANG Feng,HU Shengtao,ZHANG Erbing,SONG Houpan,CHEN Cong,CAI Xiong,LIAO Jing |
| (Institute of Innovation and Applied Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Key Laboratory of Translational Research in TCM Formulas and Zheng, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To establish an animal model of "arthralgia syndrome" in traditional Chinese medicine (TCM) based on the theory of testing syndrome by formula, which was consistent with the pathological characteristics and pathogenesis of rheumatoid arthritis (RA). Methods 40 male Sprague-Dawely (SD) rats were randomly assigned into the blank group, the wind cold dampness arthralgia syndrome model group, wind cold dampness arthralgia syndrome model+Wutou Decoction group, wind cold dampness arthralgia syndrome model+Simiao Pill group, with 10 rats in each group. There was no intervention in the blank group, the rats in other groups were stimulated by wind, cold and wet environment for 14 days, and then induced to establish adjuvant arthritis (AIA) rat model. At the same time, the wind cold dampness arthralgia syndrome model+Wutou Decoction group, wind cold dampness arthralgia syndrome model+Simiao Pill group were given Wutou Decoction and Simiao Pill by gavage until the end of the experiment. The onset time and incidence rate of rats were recorded. Foot swelling volume and arthritis index score were measured dynamically. HE staining was used to observe the pathological changes of the ankle joint in rats. CT was used to detect the fracture of bone and joint in rats. Results Compared with the wind cold dampness arthralgia syndrome model group, Wutou Decoction significantly delayed the onset time of rats and reduce the incidence of a disease, significantly reduce index score, joint swelling rate and arthritis of rats significantly inhibit the synovial tissue and bone damage (P<0.01), and the lesion degree of wind cold dampness arthralgia syndrome model+Simiao Pill group and the wind cold dampness arthralgia syndrome model group was no significant difference (P>0.05). Conclusion Based onthe theory of testing syndrome by formula, we successfully reproduce the characteristics and pathogenesis of RA and pathological changes of RA wind cold dampness arthralgia syndrome in the rat model of combined disease for system in-depth study in the treatment of RA certificate corresponding to molecular mechanism laid a foundation. |
| Key words: rheumatoid arthritis wind cold dampness arthralgia syndrome correspondence of formula-Zheng adjuvant-induced arthritis Wutou Decoction Simiao Pill |
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