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肖荣,赵澄,张香港,吴涛,魏科,胡珏,卢芳国,秦裕辉.湖南预防“新冠肺炎”2号方对免疫缺陷小鼠模型的免疫调节作用研究[J].湖南中医药大学学报英文版,2021,41(3):333-337.[Click to copy
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| 湖南预防“新冠肺炎”2号方对免疫缺陷小鼠模型的免疫调节作用研究 |
| 肖荣,赵澄,张香港,吴涛,魏科,胡珏,卢芳国,秦裕辉 |
| (湖南中医药大学, 湖南 长沙 410208;湖南人文科技学院, 湖南 娄底 417000) |
| 摘要: |
| 目的 探讨湖南预防“新冠肺炎”2号方（简称“2号方”）的免疫调节作用与可能机制。方法 将30只BALB/c小鼠随机分为正常对照组、模型对照组、2号方干预组，每组10只。腹腔注射环孢菌素A（cyclosporin A，CsA）建立免疫缺陷小鼠模型，经灌胃给临床等效剂量的2号方水煎剂干预7 d后，常规法检测胸腺指数、脾脏指数、肺指数，ELISA法检测血清中IL-2、IFN-γ、TNF-α表达水平以及肺组织匀浆液中血管紧张素转化酶2（angiotensin converting enzyme 2，ACE2）和血管紧张素Ⅱ（angiotensin Ⅱ， AngⅡ）表达水平。结果 （1）与正常对照组比较，模型对照组小鼠胸腺指数显著降低（P<0.01），胸腺组织萎缩、出现明显的病理改变，并伴有血清中的IL-2、IFN-γ表达水平和肺组织匀浆液中ACE2含量显著降低（P<0.05）以及血清中TNF-α表达水平和肺组织匀浆液AngⅡ表达水平显著上升（P<0.05或P<0.01）；（2）与模型对照组比较，2号方干预组小鼠胸腺指数显著升高（P<0.01）、胸腺组织得到明显的改善，血清中IFN-γ表达水平显著提高（P<0.01），肺组织匀浆中AngⅡ表达水平均显著降低（P<0.01），脾脏指数和肺脏指数差异无统计学意义（P>0.05）。结论 2号方可能通过改善胸腺组织结构、调节IFN-γ、AngⅡ的表达水平而影响CsA所致免疫缺陷小鼠模型的免疫功能。 |
| 关键词: 免疫缺陷 新冠肺炎 免疫调节 2号方 炎性因子 |
| DOI：10.3969/j.issn.1674-070X.2021.03.002 |
| Received:November 11, 2020 |
| 基金项目:国家自然科学基金项目（82074250，81774126）；湖南省自然科学基金项目（2020JJ4063）；长沙市杰出创新青年人才培养计划项目（2019-23）；湖南中医药大学中医药防治新型冠状病毒肺炎科研专项项目（2020XGXM03，2020XGXM02）；湖南省高校科技创新团队《感染性疾病中医药防治研究》资助项目（15）；湖南中医药大学一流学科《基础医学》资助项目（1）。 |
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| Study on Immunomodulatory Effects of Hunan No.2 Preventive Prescription for “COVID-19” on Immunodeficiency Mouse Model |
| XIAO Rong,ZHAO Cheng,ZHANG Xianggang,WU Tao,WEI Ke,HU Jue,LU Fangguo,QIN Yuhui |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Institute of Humanities and Technology, Loudi, Hunan 417000, China) |
| Abstract: |
| Objective To explore the immunomodulatory effects and possible mechanisms of Hunan No.2 prevention prescription of "COVID-19" (No.2 prescription"). Methods 30 BALB/c mice were randomly divided into normal control group, model control group and No.2 prescription intervention group, with 10 mice in each group. The immune deficiency model of mice was established by intraperitoneal injection of cyclosporin A (CsA). After 7 days of intervention with clinical equivalent dose of decoction of No.2 prescription, thymus index, spleen index and lung index were detected by routine method, and the contents of IL-2, IFN-γ and TNF-α in serum and the levels of angiotensin converting enzyme 2 (ACE2) and angiotensin Ⅱ (AngⅡ) in lung homogenate were detected by ELISA. Results (1) Compared with the normal control group, the thymus index of the model control group was significantly decreased (P<0.01), the thymus tissue atrophy and obvious pathological changes were observed, and the levels of IL-2 and IFN-γ in serum and as well as ACE 2 in lung tissue homogenate were significantly decreased (P<0.05). Meanwhile, the expressions of TNF-α in serum and AngⅡ in lung tissue homogenate were significantly increased (P<0.05 or P<0.01). (2) Compared with the model control group, the thymus index of No.2 prescription intervention group was significantly increased (P<0.01), thymus tissue got obvious improvement, IFN-γ expression level in serum increased significantly (P<0.01), the level of Ang Ⅱ in lung tissue homogenate was significantly decreased (P<0.01). There were no statistical differences in spleen index and lung index (P>0.05). Conclusion No.2 prescription may affect the immune function of CsA induced immunodeficiency mice by improving the structure of thymus and regulating the expression levels of IFN-γ and AngⅡ. |
| Key words: immunodeficiency COVID-19 immunomodulatory No.2 prescription inflammatory factors |
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