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姜雄,李木清,毛滔,刘梨,程学荣,杨振武,杜攀攀.象皮生肌膏对慢性创面愈合及PTEN、p-AKT、VEGF蛋白表达的影响[J].湖南中医药大学学报英文版,2021,41(1):67-71.[Click to copy
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| 象皮生肌膏对慢性创面愈合及PTEN、p-AKT、VEGF蛋白表达的影响 |
| 姜雄,李木清,毛滔,刘梨,程学荣,杨振武,杜攀攀 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第二附属医院, 湖南 长沙 410005;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 通过研究象皮生肌膏对大鼠慢性难愈性创面模型PTEN、p-AKT、VEGF蛋白表达的影响，探讨象皮生肌膏促进创面愈合的作用机制。方法 从72只SD雄性大鼠中随机选取18只为空白组，其余54只大鼠使用全层皮肤缺损法制备慢性难愈性创面模型，并随机分为实验组、对照组、模型组，每组18只。造模完成后实验组予象皮生肌膏外敷，对照组予重组牛碱性成纤维细胞生长因子凝胶外敷，模型组和空白组予生理盐水外涂，于造模后的第3、7、14天观察创面愈合情况，计算创面愈合率，HE染色观察皮肤组织形态，ELISA检测各组皮肤组织PTEN、p-AKT、VEGF蛋白表达情况。结果 第3、7、14天实验组、对照组创面愈合率均高于模型组（P<0.05），且第7天实验组优于对照组（P<0.05）。HE染色结果显示，空白组第3、7、14天皮肤组织完整，前后无明显形态学变化；模型组第3、7天创面组织大量炎性细胞浸润、表皮大量坏死脱落，第14天见少量毛细血管形成；实验组及对照组第3天炎性细胞浸润、表皮灶性坏死脱落，第7、14天炎性细胞浸润逐渐减少、较多成纤维细胞和毛细血管增生、肉芽组织基本形成。第3、7、14天空白组PTEN、p-AKT、VEGF蛋白表达水平均无明显差异（P>0.05）。第3、7、14天模型组PTEN蛋白表达水平均低于空白组（P<0.05），实验组及对照组均低于模型组（P<0.05），且第7天达到最低水平（P<0.05）。第3、7、14天模型组p-AKT蛋白表达水平均高于空白组（P<0.05），实验组及对照组均高于模型组（P<0.05），且第7天达到最高水平（P<0.05）。第3、7、14天模型组VEGF蛋白表达水平均低于空白组（P<0.05），实验组及对照组均高于模型组（P<0.05），且第7天达到最高水平（P<0.05）。结论 象皮生肌膏能降低大鼠创面中PTEN蛋白表达水平、提高p-AKT、VEGF蛋白表达水平，加快慢性创面愈合速度，调控PTEN/AKT/VEGF信号通路可能是象皮生肌膏促进慢性创面愈合的机制之一。 |
| 关键词: 象皮生肌膏 慢性难愈性创面 PTEN p-AKT VEGF |
| DOI：10.3969/j.issn.1674-070X.2021.01.012 |
| Received:October 01, 2020 |
| 基金项目:湖南省科技厅临床医疗技术创新引导项目（2018SK51101）。 |
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| Effect of Xiangpi Shengji Ointment on Chronic Wound Healing and Expression of PTEN, p-AKT and VEGF |
| JIANG Xiong,LI Muqing,MAO Tao,LI Li,CHENG Xuerong,YANG Zhenwu,DU Panpan |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To study the effect of Xiangpi Shengji Ointment on PTEN, p-AKT and VEGF protein expression in rat chronic wound model, and to explore the mechanism of Xiangpi Shengji Ointment in promoting wound healing. Methods 18 SD male rats were randomly selected as the blank group, and the remaining 54 rats were made into chronic refractory wound model by full-thickness skin defect method, and were randomly divided into the experimental group, the control group and the model group, with 18 rats in each group. After modeling, the experimental group was externally applied with Xiangpi Shengji Ointment, the control group was externally applied with recombinant bovine basic fibroblast growth factor gel, and the model group and the blank group were externally coated with normal saline. The wound healing was observed on the 3rd, 7th and 14th day after modeling, the wound healing rate was calculated, the tissue morphology was observed by HE staining, and the expressions of PTEN, p-AKT and VEGF protein in skin tissue in each group were detected by ELISA. Results On the 3rd, 7th and 14th day, the wound healing rate of the experimental group and the control group was higher than the model group (P<0.05), the experimental group was better than the control group on the 7th day (P<0.05). The results of HE staining showed that on the 3rd, 7th and 14th day, the skin tissue in the blank group was intact, and there was no obvious morphological change before and after. In the model group, a large number of inflammatory cells infiltrated into the wound tissue on the 3rd and 7th day, a large number of epidermal necrosis and exfoliation, and a few capillaries formed on the 14th day; in the experimental group and the control group, inflammatory cells infiltrated, epidermal focal necrosis and exfoliationon the 3rd day, and inflammatory cells infiltration gradually decreased, with more fibroblasts and capillaries proliferating, and granulation tissue basically formed on the 7th and 14th day. The expression levels of PTEN, p-AKT and VEGF protein showed that there were no significant difference in the blank group on the 3rd, 7th and 14th day (P>0.05). On the 3rd, 7th and 14th day, the level of PTEN proteinin the model group was lower than the blank group (P<0.05), the experimental group and the control group were lower than the model group (P<0.05), and reached the lowest level on the 7th day (P<0.05). On the 3rd, 7th and 14th day, the level of p-AKT protein in the model group was higher than the blank group (P<0.05), the experimental group and the control group were higher than the model group (P<0.05), and reached the highest level on the 7th day (P<0.05). On the 3rd, 7th and 14th day, the expressionof VEGF protein in the model group was lower than the blank group (P<0.05), the experimental group and the control group was higher than the model group (P<0.05), and reached the highest level on the 7th day (P<0.05). Conclusion Xiangpi Shengji Ointment can reduce the expression level of PTEN protein, increase the expression levels of p-AKT and VEGF protein, and accelerate the speed of chronic wound healing. Regulating PTEN/AKT/VEGF signal pathway may be one of the mechanisms of Xiangpi Shengji Ointmentin promoting chronic wound healing. |
| Key words: Xiangpi Shengji Ointment chronic refractory wound PTEN p-AKT VEGF |
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