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陈晓莹,何昶昊,张晓萌,王磊,石中玉,葛东宇,董瑞娟,闫玥,叶一帆,胡素敏.益气解毒方对2Gy 60Coγ射线诱导的雄性小鼠生精功能损伤的防护作用[J].湖南中医药大学学报英文版,2020,40(12):1456-1461.[Click to copy
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| 益气解毒方对2Gy 60Coγ射线诱导的雄性小鼠生精功能损伤的防护作用 |
| 陈晓莹,何昶昊,张晓萌,王磊,石中玉,葛东宇,董瑞娟,闫玥,叶一帆,胡素敏 |
| (北京中医药大学中医学院, 北京 100029) |
| 摘要: |
| 目的 探究2Gy 60Coγ射线对雄性小鼠睾丸的损伤以及益气解毒方对睾丸辐射损伤的防护效应。方法 将60只雄性BALB/c小鼠随机分为空白组、模型组、安多霖组以及益气解毒方高、中、低剂量组，每组10只。预防性给药7 d后，除空白组小鼠外，其余各组小鼠均给予2Gy 60Coγ射线一次性全身照射，并于照射后第4天持续给药至第34天，给药期间对所有小鼠进行体质量监测，照射后第35天取材检测小鼠全血细胞，记录睾丸和附睾质量，HE染色观察睾丸及附睾形态结构的变化，并对小鼠睾丸生精上皮进行半定量分析。结果 照射后第4、15天，模型组小鼠体质量较空白组均明显下降（P<0.05，P<0.01），而各给药组小鼠体质量下降趋势不明显，至照射后第35天各照射组体质量恢复至空白组水平（P>0.05）。照射后第35天，与空白组比较，模型组小鼠全血细胞、睾丸和附睾质量显著下降（P<0.05，P<0.01）；睾丸生精小管损伤严重，生精上皮厚度显著变薄（P<0.01）；附睾管腔内空泡化变性明显，精子数显著下降。与模型组比较，益气解毒方中剂量组小鼠淋巴细胞、红细胞、血小板数量及睾丸质量均明显升高（P<0.05），附睾质量差异无统计学意义（P>0.05）；睾丸及附睾病理改变均明显改善，且其生精上皮厚度均显著恢复（P<0.01）。结论 预防加治疗性给予益气解毒方能够有效改善电离辐射后雄性小鼠睾丸损伤，促进生精上皮再生，且该方中剂量的效果更优。 |
| 关键词: 益气解毒方 电离辐射 睾丸损伤 生精功能 |
| DOI：10.3969/j.issn.1674-070X.2020.12.004 |
| Received:November 06, 2020 |
| 基金项目:国家自然科学基金项目（11675027，12075035）；北京中医药大学科研发展基金项目（2019-ZXFZJJ-009）；北京中医药大学重点攻关项目（2020-JYB-ZDGG-008）。 |
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| Protective Effect of Yiqi Jiedu Prescription on Injury of Male Mice Spermatogenic Function Induced by 2Gy 60Coγ Rays |
| CHEN Xiaoying,HE Changhao,ZHANG Xiaomeng,WANG Lei,SHI Zhongyu,GE Dongyu,DONG Ruijuan,YAN Yue,YE Yifan,HU Sumin |
| (School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China) |
| Abstract: |
| Objective To explore injury caused by 2Gy 60Coγ rays in testes of male mice and the protective effect of Yiqi Jiedu Prescription on radiation injury in testes. Methods Sixty male BALB/c mice were randomly assigned into a blank group, a model group, an Anduolin group, and high-, medium-, and low-dose Yiqi Jiedu Prescription groups, with 10 mice in each group. After 7 days of preventive administration, except for the mice in the blank group, the mice in the other groups were given one-time whole body irradiation with 2Gy 60Coγ rays, and the administration was continued from the 4th day to the 34th day. All the mice were monitored for body mass during the administration. On the 35th day after the irradiation, samples were taken to detect mouse whole blood cells, to record mass of testes and epididymides, and to observe changes in morphology and structure of the testes and epididymides by HE staining. Semi-quantitative analysis on mouse testis seminiferous epithelium was performed. Results The body mass of mice in the model group was significantly lower than that in the blank group on the 4th and 15th days after the irradiation (P<0.05, P<0.01), while the decline in the body mass of the mice in each administration group was not obvious. Until the 35th day after the irradiation, the body mass in each irradiation group returned to the level in the blank group (P<0.05). On the 35th day after the irradiation, compared with the blank group, the mass of whole blood cells, testes and epididymides in the model group was decreased significantly (P<0.05, P<0.01); testicular seminiferous tubules were seriously injured, and the thickness of the seminiferous epithelium was significantly thinner (P<0.01); vacuolation and degeneration in the epididymal lumen was obvious, and the sperm count was significantly decreased. Compared with the model group, the numbers of lymphocytes, red blood cells and platelets and the testes mass in the medium-dose Yiqi Jiedu Prescription group were significantly increased (P<0.05), and there was no statistically significant difference in the epididymal mass (P>0.05); The pathological changes of the testes and epididymides were significantly improved, and the thickness of the seminiferous epithelium was significantly restored (P<0.01). Conclusion Preventive and therapeutic administration of Yiqi Jiedu Prescription can effectively improve the testes injury in male mice after ionizing radiation and promote regeneration of seminiferous epithelium, and the medium dose of this prescription has a better efficacy. |
| Key words: Yiqi Jiedu Prescription ionizing radiation testicular injury spermatogenic function |
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