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谢赛飞,谭劲,郑玲,朱可可,周领航,陈明.扶正活血解毒方通过Wnt/β-catenin信号通路对口腔黏膜下纤维化的调控作用[J].湖南中医药大学学报英文版,2020,40(11):1305-1309.[Click to copy
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| 扶正活血解毒方通过Wnt/β-catenin信号通路对口腔黏膜下纤维化的调控作用 |
| 谢赛飞,谭劲,郑玲,朱可可,周领航,陈明 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 探索扶正活血解毒方对槟榔碱提取物（areca nut extract，ANE）诱导的口腔黏膜下纤维化（oral submucous fibrosis，OSF）Wnt/β-catenin信号通路的作用机制。方法 体外培养大鼠口腔黏膜上皮细胞（epithelial cell，EC），分为正常组、模型组、中药高/中/低剂量组和IWR-1组。以ANE为诱导剂，扶正活血解毒方含药血清为干预药物，以Wnt/β-catenin信号通路抑制剂IWR-1为阳性对照物，采用CCK8检测EC细胞增殖；PCR检测Wnt1、β-catenin、Axin、cylinD1基因的表达；Western blot检测Wnt1、β-catenin、Axin、cylinD1蛋白的表达。结果 与正常组相比，模型组EC增殖水平提高，Wnt1、β-catenin、cylinD1基因及蛋白表达增加，Axin基因及蛋白表达降低（P<0.05）；与模型组相比，中药高、中剂量组EC增殖水平降低，Wnt1、β-catenin、cylinD1基因及蛋白表达降低，Axin基因及蛋白表达升高（P<0.05）；与模型组相比，IWR-1组EC增殖水平降低，Wnt1、β-catenin、cylinD1基因及蛋白表达降低，Axin基因及蛋白表达升高（P<0.05）。结论 扶正活血解毒方可通过调控Wnt/β-catenin信号通路，抑制ANE刺激的EC增殖，逆转OSF癌前病变的形成。 |
| 关键词: 口腔黏膜下纤维化 槟榔碱提取物 扶正活血解毒 Wnt/β-catenin信号通路 |
| DOI：10.3969/j.issn.1674-070X.2020.11.001 |
| Received:June 15, 2020 |
| 基金项目:国家自然科学基金项目（81874496）；湖南省中医药管理局课题（201808）；湖南中医药大学研究生创新课题（2019CX73）。 |
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| Regulating Effect of Fuzheng Huoxue Jiedu Prescription on Oral Submucosal Fibrosis Through Wnt/β-catenin Signaling Pathway |
| XIE Saifei,TAN Jin,ZHEN Ling,ZHU Keke,ZHOU Linghang,CHEN Ming |
| (The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To explore the mechanism of Fuzheng Huoxue Jiedu Prescription on Wnt/β-catenin signaling pathway induced by areca nut extract (ANE) in oral submucosal fibrosis. Methods The rat oral mucosa epithelial cells (ECs) cultured in vitro were assigned into a normal group, a model group, a Chinese materia medica high, medium and low dose group, a IWR-1 group. ANE was inducer, and Fuzheng Huoxue Jiedu Prescription-containing serum was intervention medicine. Wnt/β-catenin signaling pathway inhibitor IWR-1 was the positive control medicine. The CCK8 was used to test EC cell proliferation; PCR was used to detect Wnt1, β- catenin, Axin, cylinD1 gene expression; Western Blot was used to detect Wnt1, β-catenin, Axin, cylinD1 protein expression. Results Compared with the normal group, ECs proliferation levels of the model group increased, and Wnt1, β-catenin, cylinD1 gene and protein expression increased. Axin gene and protein expression decreased (P<0.05); Compared with model group, ECs proliferation levels of Chinese materia medica high and medium dose groups were reduced, and Wnt1, β-catenin, cylinD1 gene and protein expression were reduced. Axin gene and protein expression increased (P<0.05); Compared with the model group, ECs proliferation level of the IWR-1 group was reduced, and Wnt1, β-catenin, cylinD1 gene and protein expression were reduced. Axin gene and protein expression increased (P<0.05). Conclusion Fuzheng Huoxue Jiedu Prescription can inhibit the proliferation of EC cells stimulated by ANE, and reverse the formation of OSF precancerous lesions through regulating Wnt/β-catenin signaling pathway. |
| Key words: oral submucosal fibrosis areca nut extract Fuzheng Huoxue Jiedu Wnt/β-catenin signal pathway |
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