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魏露,朱明芳,吴淑辉,刘娟,张曦.石榴皮多酚乳膏对金黄地鼠皮脂腺斑组织中AMPK/ACC-1信号通路的影响[J].湖南中医药大学学报英文版,2020,40(10):1221-1225.[Click to copy
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| 石榴皮多酚乳膏对金黄地鼠皮脂腺斑组织中AMPK/ACC-1信号通路的影响 |
| 魏露,朱明芳,吴淑辉,刘娟,张曦 |
| (湖南中医药大学第二附属医院, 湖南 长沙 410005) |
| 摘要: |
| 目的 通过观察石榴皮多酚乳膏对金黄地鼠皮脂腺斑腺苷酸活化蛋白激酶（AMP-activated protein kinase，AMPK）、乙酰辅酶A羧化酶-1（acetyl-COA carboxylase-1，ACC-1）的影响，研究外用石榴皮多酚乳膏治疗痤疮的作用机制。方法 将24只雄性金黄地鼠随机分成4组，分别是空白组、基质组、石榴皮多酚乳膏组、阳性对照组，每组6只，分别于双侧皮脂腺斑处涂抹蒸馏水、基质、石榴皮多酚乳膏、0.025%维A酸乳膏，连续给药4周，2次/d。观察各组金黄地鼠皮脂腺斑面积变化，并取材观察组织病理学变化，免疫组化法检测各组AMPK、ACC-1的表达水平。结果 与空白组和基质组相比，各治疗组皮脂腺体积变小，数量减少，排列疏松；AMPK水平均明显升高，差异有统计学意义（P<0.05）；ACC-1水平明显降低，差异有统计学意义（P<0.05）。与阳性对照组相比，石榴皮多酚乳膏组ACC-1水平降低，差异有统计学意义（P<0.05）。结论 石榴皮多酚乳膏治疗痤疮的作用机制可能与激活AMPK/ACC-1信号通路表达，从而改善皮脂稳态失衡有关。 |
| 关键词: 石榴皮多酚乳膏 痤疮 皮脂稳态失衡 腺苷酸活化蛋白激酶 乙酰辅酶A羧化酶-1 |
| DOI：10.3969/j.issn.1674-070X.2020.10.009 |
| Received:June 10, 2020 |
| 基金项目:湖南省教育厅科学研究项目（18A212）；湖南省临床医疗技术创新引导项目（2018SK51102）；湖南省研究生科研创新项目（CX2018B501）；湖南中医药大学研究生创新项目（2019CX03）；湖南省卫生健康委2020年度科研计划项目（20201862）；湖南省高层次卫生人才“225”工程培养项目资助。 |
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| Effects of Pomegranate Peel Polyphenol Cream on AMPK/ACC-1 Signaling Pathway in of Golden Hamsters' Flank Organs |
| WEI Lu,ZHU Mingfang,WU Shuhui,LIU Juan,ZHANG Xi |
| (The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China) |
| Abstract: |
| Objective To observe the effect of pomegranate peel polyphenol cream on AMP-activated protein kinase (AMPK) and acetyl-COA carboxylase-1 (ACC-1) signaling pathway in golden hamsters' flank organs, and to explore the action mechanism of pomegranate peel polyphenol cream in the treatment of acne. Methods A total of 24 female golden hamsters were randomly divided into 4 groups:a blank group, a matrix group, a pomegranate peel polyphenol cream group and a positive control group, with 6 golden hamsters in each group. Distilled water, matrix, pomegranate polyphenol cream and 0.025% Vitamin A cream were applied to bilateral flank organs, twice a day for 4 weeks. After continuous treatment for 4 weeks, the change of golden hamsters' flank organs in each group was observed. The histopathologic changes were observed after collecting materials. The expression level of AMPK and ACC-1 in local tissue was tested by immunohistochemistry method. Results Compared with the blank group and the matrix group, the size and quantity of sebaceous glands in each treatment group were reduced, and arranged loosely. The level of AMPK in each treatment group were significantly higher than the blank group and the matrix group, and the difference was statistically significant (P<0.05). The level of ACC-1 in each treatment group was significantly lower than the blank group and the matrix group, and the difference was statistically significant (P<0.05). Compared with the positive control group, the level of ACC-1 in pomegranate peel polyphenol cream were significantly decreased, and the difference was statistically significant (P<0.05). Conclusion The mechanism of pomegranate peel polyphenol cream in acne treatment may be related to the activation of AMPK/ACC-1 signaling pathway expression, thus improving sebum homeostasis imbalance. |
| Key words: pomegranate peel polyphenol cream acne sebum homeostasis imbalance AMP-activated protein kinase acetyl-COA carboxylase-1 |
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