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欧阳波,刘晓丹,杨筱倩,丁煌,唐三,黄小平,邓常清.冰片配伍黄芪甲苷和三七总皂苷对大鼠脑缺血再灌注损伤神经元的保护作用[J].湖南中医药大学学报英文版,2020,40(10):1210-1215.[Click to copy
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| 冰片配伍黄芪甲苷和三七总皂苷对大鼠脑缺血再灌注损伤神经元的保护作用 |
| 欧阳波,刘晓丹,杨筱倩,丁煌,唐三,黄小平,邓常清 |
| (湖南中医药大学分子病理实验室, 中西医结合心脑疾病防治湖南省重点实验室, 细胞生物学与分子技术湖南省高校重点实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨冰片配伍黄芪甲苷（astragaloside Ⅳ，AST Ⅳ）和三七总皂苷（panax notoginseng saponins，PNS）对大鼠脑缺血再灌注损伤（cerebral ischemia reperfusion injury，CIRI）后神经元的保护作用。方法 成年雄性SD大鼠随机分为假手术组、模型组、冰片组、AST Ⅳ组、PNS组、AST Ⅳ+PNS组、冰片+AST Ⅳ+PNS低剂量组、冰片+AST Ⅳ+PNS高剂量组、依达拉奉组。依达拉奉组腹腔注射给药，其他组灌胃给药，2次/d。采用线栓法阻断右侧大脑中动脉（middle cerebral artery，MCA），缺血2 h再灌注22 h建立CIRI模型。行神经功能缺损评分评定神经功能；尼氏染色计数大鼠缺血侧海马CA1区细胞尼氏体数量；免疫荧光法检测缺血侧海马CA1区神经丝蛋白200（neurofilament-200，NF-200）表达；Westrn blot法检测αII-血影蛋白（αII-Spectrin，αII SP）表达。结果 CIRI后，模型组神经功能缺损评分显著升高（P<0.01），尼氏体计数显著减少（P<0.01），海马CA1区NF-200及αII SP蛋白表达均显著减少（P<0.01）。冰片＋AST Ⅳ＋PNS组可显著降低神经功能缺损评分，增加尼氏体计数量及NF-200和αII SP蛋白表达（P<0.05，P<0.01），冰片＋AST Ⅳ＋PNS配伍的作用强于各药物单用组及AST Ⅳ＋PNS组。结论 冰片、AST Ⅳ、PNS具有抑制CIRI后神经元损伤的作用，3种药物配伍效应增强。 |
| 关键词: 冰片 黄芪甲苷 三七总皂苷 脑缺血再灌注 神经元 |
| DOI：10.3969/j.issn.1674-070X.2020.10.007 |
| Received:July 08, 2020 |
| 基金项目:国家自然科学基金项目（81573875）；湖南省高校创新平台开放基金项目（14K068）；湖南省自然科学基金项目（2018JJ3382）；湖南省教育厅优秀青年项目（18B236）；湖南中医药大学“十三五”一级学科基础医学建设项目（No.06）。 |
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| Protective Effect of Borneol Combined with Astragaloside Ⅳ and Panax Notoginseng Saponins on Neurons after Cerebral Ischemia/Reperfusion Injury in Rats |
| OUYANG Bo,LIU Xiaodan,YANG Xiaoqian,DING Huang,TANG San,HUANG Xiaoping,DENG Changqing |
| (Molecular Pathology Laboratory of Hunan University of Chinese Medicine, Hunan Provincial Key Laboratory of Cardio-Brain Disease Prevention and Treatment of Integrated Traditional Chinese and Western Medicine, Hunan Provincial University Key Laboratory of Cell Biology and Molecular Technology, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the protective effect of borneol combined with Astragaloside Ⅳ and Panax notoginseng saponins on neurons after cerebral ischemia/reperfusion injury (CIRI) in rats. Methods The adult male Sprague-Dawley (SD) rats were randomly divided into a sham operation group, a model group, a borneol group, a Astragaloside Ⅳ(AST Ⅳ) group, a Panax notoginseng saponins (PNS) group, a AST Ⅳ + PNS group, a low dose (borneol + AST Ⅳ + PNS) group, a high dose (borneol + AST Ⅳ + PNS) group and a edaravone group. The edaravone group was intraperitoneally injected and the other groups were administered by intragastric administration, twice a day. The right middle cerebral artery (MCA) was blocked by wire bolt, and the CIRI model was established after ischemia for 2 hours and reperfusion for 22 hours. The neurological function scores were scored and the number of Nissl bodies in the CA1 area of the right hippocampus of rats were observed by Nissl staining. The protein expressions of neurofilament-200 (NF-200) in the CA1 area of the right brain hippocampus were detected by immunofluorescence. The expressions of αII-Spectrin (αII SP) were detected by Western blot. Results After CIRI, the score of neural dysfunction in the model group was significantly increased (P<0.01), and the number of Nissl body decreased significantly (P<0.01). The expressions of NF-200 and αIISP protein in hippocampus CA1 area were significantly reduced (P<0.01). Borneol + AST IV + PNS could significantly reduce the score of nerve function defect, and increased the number of Nissl body, the expression of NF-200 and αII SP protein (P<0.05 and P<0.01). The borneol + AST IV + PNS effect was better than that of single drug and AST IV + PNS. Conclusion Borneol, AST Ⅳ, and PNS have anti-neuronal damage after cerebral ischemia reperfusion injury, and the effect of the combination of the three drugs was enhanced. |
| Key words: borneol astragaloside IV panax notoginseng saponins cerebral ischemia reperfusion neurons |
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