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葛冰景,曹红燕,戴彦成,阙任烨,傅志泉.白芍总苷对葡聚糖硫酸钠诱导的IBD模型小鼠炎症因子水平的影响[J].湖南中医药大学学报英文版,2020,40(8):957-963.[Click to copy
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| 白芍总苷对葡聚糖硫酸钠诱导的IBD模型小鼠炎症因子水平的影响 |
| 葛冰景,曹红燕,戴彦成,阙任烨,傅志泉 |
| (上海中医药大学附属上海市中西医结合医院, 上海 200082) |
| 摘要: |
| 目的 探讨白芍总苷对葡聚糖硫酸钠（dextran sulfate sodium,DSS）诱导的小鼠炎症性肠病（inflammatory bowel disease,IBD）模型的作用及相关机制研究。方法 将50只SPF级C57BL/6小鼠随机分成正常组（N组）、模型组（M组）、柳氮磺吡啶组（L组）、低剂量白芍总苷组（BL组）、高剂量白芍总苷组（BH组），每组10只。M组及治疗组小鼠自由饮用30 g/L DSS，同时予以相应药物灌胃1次；N组自由饮水、进食，连续7 d后处死。期间每天记录小鼠体质量及粪便情况，进行结肠炎疾病活动指数（disease activity index,DAI）评分，利用结肠组织HE染色进行组织病理学评价，利用ELISA法检测血清中IL-8、IL-10、IL-23、IL-36、CCL20、CCR6的表达。结果 与M组比较，用药组DAI评分均显著降低（P<0.05或P<0.01），各用药组之间差异无统计学意义（P>0.05）；与M组比较，用药组均可下调IL-8、IL-23、IL-36、CCL20和CCR6的表达，上调IL-10的表达，并以L组、BH组最为显著（P<0.05或P<0.01）；病理组织学结果显示，L组、BH组结肠组织的炎症反应得到了显著改善。结论 白芍总苷可以有效调节炎症因子表达，减少结肠组织的炎症损伤，并改善小鼠的腹泻及便血症状。 |
| 关键词: 白芍总苷 炎症性肠病 葡聚糖硫酸钠 炎症因子 疾病活动指数 |
| DOI：10.3969/j.issn.1674-070X.2020.08.010 |
| Received:December 17, 2019 |
| 基金项目:国家自然科学基金面上项目（81873253）；上海市自然科学基金项目（16ZR1433800）；上海市中西医结合医院人才引进专项基金项目（160304）。 |
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| Effects of Total Glucosides of Paeony on Inflammatory Factors in Dextran Sulfate Sodium Induced-Mice Model of Inflammatory Bowel Disease |
| GE Bingjing,CAO Hongyan,DAI Yancheng,QUE Renye,FU Zhiquan |
| (Shanghai Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Chinese Medicine, Shanghai 200082, China) |
| Abstract: |
| Objective To explore the effect and the underlying mechanism of total glucosides of paeony on inflammatory factors in dextran sulfate sodium (DSS) induced-mice model of inflammatory bowel disease (IBD). Methods A total of 50 SPF-class C57BL/6 mice were randomly divided into a normal group (N group), a model group (M group), a sulfasalazine group (L group), a low-dose of total glucosides of paeony group (BL group) and high-dose of total glucosides of paeony group (BH group), with 10 mice in each group. The mice in M group and treatment groups were free to drink 30 g/L DSS, and were given the corresponding medicine by gavage once, while the mice in the N group were free to drink water and eat. All the mice were executed 7 days later. During this period, the status of weight and feces of the mice were recorded, and colitis disease activity index (DAI) score were assessed. In addition, HE staining of colon tissue were performed to evaluate the histopathological injury, and the expression of serum IL-8, IL-10, IL-23, IL-36, CCL20 and CCR6 were determined by using ELISA. Results Compared with the M group, DAI scores of mice in the treatment group were significantly decreased(P<0.05 or P<0.01), and the differences in each treatment group was not statistically significant (P>0.05). Compared with the M group, the expression of IL-8, IL-23, IL-36, CCl20, and CCR6 were decreased in the treatment group, while the expression of IL-10 was increased, in which the L group and the BH group were most significant (P<0.05 or P<0.01). The histopathological results showed that the inflammatory response of colon tissue in the L group and the BH group was significantly improved. Conclusion Total glucosides of paeony could effectively regulate the expression of inflammatory factors, reduce the inflammatory injury of colon tissues, and improve the diarrhea and hematochezia symptoms in mice. |
| Key words: total glucosides of paeony inflammatory bowel disease dextran sulfate sodium inflammatory factor disease activity index |
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