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唐雪,李波男,周海亮,何清湖,周青,熊伟,高瑞松,林群芳,周兴.雄蚕益肾方对迟发性性腺功能减退症大鼠睾丸组织形态学改变及抗氧化作用研究[J].湖南中医药大学学报英文版,2020,40(8):951-956.[Click to copy
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| 雄蚕益肾方对迟发性性腺功能减退症大鼠睾丸组织形态学改变及抗氧化作用研究 |
| 唐雪,李波男,周海亮,何清湖,周青,熊伟,高瑞松,林群芳,周兴 |
| (湖南中医药大学第二附属医院, 湖南 长沙 410005;湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学第一附属医院, 湖南 长沙 410007;东部战区总医院, 江苏 南京 210002) |
| 摘要: |
| 目的 探讨雄蚕益肾方对迟发性性腺功能减退症（lateonset hypogonadism,LOH）大鼠睾丸组织形态学改变及抗氧化作用。方法 将18月龄SD雄性大鼠随机分为模型组，丙酸睾酮组，雄蚕益肾方低、中、高剂量组，2月龄SD雄性大鼠设为正常组。雄蚕益肾方低、中、高剂量组分别给予10.4、20.8、41.6 g/kg的雄蚕益肾方配方颗粒剂灌胃；丙酸睾酮组肌注5.21 mg/kg丙酸睾酮，隔日1次；正常组和模型组给予相同体积的蒸馏水灌胃，均连续28 d。实验结束后，记录大鼠悬尾实验不动时间，称体质量、采血，ELSIA法检测睾酮（T），睾丸组织进行黄嘌呤氧化酶法（SOD）、紫外吸收法（CAT）、硫代巴比妥酸法（MDA）、病理及电镜检测。结果 与正常组比较，模型组T水平降低（P<0.05），悬尾不动时间延长（P<0.05），睾丸组织抗氧化酶SOD、CAT降低（P<0.05）、MDA升高（P<0.05），HE染色睾丸间质减少，电镜示Leydig细胞线粒体数量减少、水肿明显，均符合LOH改变。与模型组比较，雄蚕益肾方各剂量组和丙酸睾酮组均能提高T水平（P<0.05）、缩短悬尾不动时间（P<0.05），雄蚕益肾方中、高剂量组和丙酸睾酮组均能升高SOD、CAT（P<0.05），降低MDA（P<0.05）。雄蚕益肾方各剂量组大鼠睾丸组织生精小管内精子数量增多，睾丸间质增加；电镜示Leydig细胞线粒体数量明显增多，部分可见线粒体鞘，部分线粒体嵴清晰，线粒体水肿不明显。结论 雄蚕益肾方能提升LOH大鼠T水平、缩短悬尾不动时间、一定程度改变睾丸组织形态和超微结构，可能与提高睾丸组织抗氧化能力有关。 |
| 关键词: 男性迟发性性腺功能减退症 雄蚕益肾方 睾酮 组织形态 抗氧化 |
| DOI：10.3969/j.issn.1674-070X.2020.08.009 |
| Received:November 12, 2019 |
| 基金项目:国家自然科学基金（81673984）；湖南省自然科学基金项目（2018JJ3402）；长沙市杰出创新青年培养计划项目（kq1802015）；湖南省高层次卫生人才225工程医学学科骨干人才培养对象（湘卫函〔2019〕196号）；湖南省创新型省份建设专项经费“湖湘青年英才”计划（2019RS2041）；中华中医药学会“青年人才托举工程”（2019-QNRC2-C07）。 |
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| Study on the Effects of Xiongcan Yishen Recipe on the Histomorphology and Antioxidation of Testes in Rats with Late onset Hypogonadism |
| TANG Xue,LI Bonan,ZHOU Hailiang,HE Qinghu,ZHOU Qing,XIONG Wei,GAO Ruisong,LIN Qunfang,ZHOU Xing |
| (The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Eastern Theater General Hospital, Nanjing, Jiangsu 210002, China) |
| Abstract: |
| Objective To study the effects of Xiongcan Yishen Recipe on testicular histomorphology and antioxidation in rats with late onset hypogonadism (LOH). Methods The 18-month-old SD male rats were randomly divided into a model group, a testosterone propionate group, a Xiongcan Yishen Recipe low, middle and high dose group. The 2-month-old SD male rats were set as normal group. Xiongcan Yishen Recipe low, middle and high dose group were given 10.4 g/kg, 20.8 g/kg and 41.6 g/kg Xiongcan Yishen Recipe granules by gavage respectively; Testosterone propionate group was intramuscularly given 5.21 mg/kg testosterone propionate, once every other day. The normal group and the model group were given the same volume of distilled water by gavage for 28 days. At the end of the experiment, the immobility time of tail suspension experiment was recorded. They were weighed, and blood was collected. Testosterone (T) was detected by ELSIA, and xanthine oxidase method was used to detect superoxide dismutase (SOD) in testicular tissue. Ultraviolet absorption method was used to detect catalase (CAT), and thiobarbituric acid method was used to detect malondialdehyde (MDA), pathology and electron microscopy. Results Compared with the normal group, T level in the model group decreased (P<0.05). Tail suspension time prolonged (P<0.05). antioxidant enzyme SOD, CAT decreased (P<0.05) and MDA increased (P<0.05) in testicular tissue. HE staining testicular stroma decreased. Electron microscopy showed that Leydig cell mitochondria decreased, and edema was obvious, which was in line with LOH change. Compared with the model group, each dose group of Xiongcan Yishen Recipe and testosterone propionate group could increase T level (P<0.05) and shorten tail suspension time (P<0.05), while SOD and CAT (P<0.05) increased, and MDA (P<0.05) was significantly decreased in the middle and high dose groups and testosterone propionate group. The number of sperm in the seminiferous tubules of the testis tissue of rats in each dose of Xiongcan Yishen Recipe increased, and the interstitium of the testis increased. Electron microscopy showed that the number of mitochondria in Leydig cells increased significantly, and some mitochondrial sheaths were visible. Some mitochondrial cristae were clear, and mitochondrial edema was not obvious. Conclusion Xiongcan Yishen Recipe can improve T level, reduce the time of tail suspension, and change the morphology and ultrastructure of testis to some extent, which may be related to improving the antioxidant capacity of testis. |
| Key words: late onset hypogonadism in male Xiongcan Yishen Recipe testosterone histomorphometric antioxidation |
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