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陈学彰,田华琴,梁贵文,王斌,李宏良,叶伟琼.癌理通膏外敷治疗癌性疼痛的机制研究[J].湖南中医药大学学报英文版,2020,40(7):841-845.[Click to copy
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| 癌理通膏外敷治疗癌性疼痛的机制研究 |
| 陈学彰,田华琴,梁贵文,王斌,李宏良,叶伟琼 |
| (广州中医药大学附属佛山市中医院肿瘤中心, 广东 佛山 528000) |
| 摘要: |
| 目的 观察癌理通膏对BALB/c癌性疼痛裸鼠的镇痛作用及对肿瘤生长、脊髓神经生长因子（NGF）、肿瘤坏死因子-α（TNF-α）、内皮素-1（ET-1）、β-内啡肽（β-EP）表达的影响。方法 通过在BALB/c裸鼠大腿外侧近臀部处皮下注射Walker 256乳腺癌细胞的方法建立裸鼠癌痛模型，动态观察癌理通组和模型组裸鼠的自发痛评分、肿瘤体积的变化，剥离肿瘤比较两组肿瘤重量的差异，RT-PCR法检测两组脊髓NGF、TNF-α、ET-1、β-EP的含量变化。结果 癌理通组裸鼠从第7天起自发痛评分明显低于模型组，持续到第10天（P<0.05），肿瘤体积从第3天起明显小于模型组，持续到第10天（P<0.01）。与模型组比较，癌理通组肿瘤重量（P<0.01）、脊髓NGF（P<0.01）及TNF-α（P<0.05）的含量均明显下降，脊髓β-EP含量明显升高（P<0.05），脊髓ET-1含量差异无统计学意义（P>0.05）。结论 癌理通膏能明显改善BALB/c裸鼠的癌性疼痛，其作用机制可能与癌理通膏抑制肿瘤的生长、降低脊髓NGF、TNF-α含量、升高脊髓β-EP的含量有关。 |
| 关键词: 癌理通膏 癌性疼痛 肿瘤坏死因子-α 神经生长因子 β-内啡肽 内皮素-1 |
| DOI：10.3969/j.issn.1674-070X.2020.07.012 |
| Received:February 24, 2020 |
| 基金项目:佛山市科技局立项课题（2017AB002261）。 |
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| Mechanism Study of External Application with Ailitong Ointment in Treating Cancer Pain |
| CHEN Xuezhang,TIAN Huaqin,LIANG Guiwen,WANG Bin,LI Hongliang,YE Weiqiong |
| (Cancer Center, Foshan Hospital of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Foshan, Guangdong 528000, China) |
| Abstract: |
| Objective To observe the analgesic effects of external application with Ailitong Ointment on BALB/c nude mouse with cancer pain and its impact on tumor growth, spinal nerve growth factor (NGF), tumor necrosis factor alpha (TNF-α), endothelin-1 (ET-1) and beta-endorphin (β-EP) expressions. Methods Walker 256 breast cancer cells were hypodermically injected in lateral thigh of BALB/c nude rats to establish nude rat model of cancer pain. The changes of spontaneous pain scores, gross tumor volume in the Ailitong group and the model group were dynamically observed. Tumor tissues were stripped to compare the tumor weight in the 2 groups; RT-PCR was used to detect the changes of spinal NGF, TNF-α, ET-1 and β-EP levels. Results From day 7 to day 10, spontaneous pain scores in the Ailitong group were significantly lower than the model group (P<0.05); From day 3 to day 10, tumor volume in the Ailitong group was significantly smaller than model group (P<0.01). Compared with the model group, the tumor weight (P<0.01), NGF levels (P<0.01) and TNF-α levels (P<0.05) in the Ailitong group were significantly decreased; β-EP levels were significantly increased (P<0.05); ET-1 levels between the 2 groups were not significantly different (P>0.05). Conclusion Ailitong Ointment can significantly relieve the cancer pain of BALB/c nude rats, whose mechanism may be correlated with Ailitong Ointment in inhibiting tumor growth, decreasing NGF and TNF-α contents and increasing β-EP levels. |
| Key words: Ailitong Ointment cancer pain tumor necrosis factor alpha spinal nerve growth factor beta-endorphin endothelin-1 |
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