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徐寅,张彧,陈末,周义方,曾蓉,郭璇,喻斌,王小娟.灭幽汤对幽门螺杆菌相关性胃炎脾胃湿热证模型小鼠FoxO3a、Bim和FasL的影响[J].湖南中医药大学学报英文版,2020,40(7):828-833.[Click to copy
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| 灭幽汤对幽门螺杆菌相关性胃炎脾胃湿热证模型小鼠FoxO3a、Bim和FasL的影响 |
| 徐寅,张彧,陈末,周义方,曾蓉,郭璇,喻斌,王小娟 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察灭幽汤对幽门螺杆菌相关性胃炎（Hp associated gastritis，HAG）脾胃湿热证模型小鼠FoxO3a、Bim和FasL的影响，探讨其对HAG脾胃湿热证的治疗机制。方法 将60只SPF级BALB/c小鼠随机分为模型组、胃四联组、低剂量灭幽汤组（灭幽低组）、中剂量灭幽汤组（灭幽中组）、高剂量灭幽汤组（灭幽高组）、正常组，共6组，每组10只。除正常组外其余5组均采用复合病因（肥甘食物+幽门螺杆菌+湿热环境）造模法建立HAG脾胃湿热证小鼠模型，造模成功后，各组予以相应干预，正常组及模型组予以蒸馏水灌胃，连续14 d。TUNEL法检测胃黏膜细胞凋亡情况，Western blot、RT-PCR分别检测胃黏膜组织FoxO3a、FasL和Bim蛋白表达及基因含量。结果 TUNEL法检测胃黏膜细胞凋亡情况：模型组较正常组细胞凋亡情况明显增多，除灭幽低组外其余治疗组细胞凋亡情况较模型组明显减少。与正常组比较，模型组FoxO3a、FasL、Bim蛋白及mRNA表达升高，差异有显著统计学意义（P<0.01）；与模型组比较，灭幽高组、灭幽中组、胃四联组FoxO3a、FasL、Bim蛋白及mRNA表达降低，差异有显著统计学意义（P<0.01）。结论 中、高剂量灭幽汤能有效治疗HAG脾胃湿热证，其机制可能是灭幽汤通过调控FoxO3a表达，下调凋亡因子FasL和Bim含量来抑制胃上皮细胞凋亡而实现。 |
| 关键词: 幽门螺杆菌相关性胃炎 灭幽汤 脾胃湿热证 细胞凋亡 FoxO3a FasL Bim |
| DOI：10.3969/j.issn.1674-070X.2020.07.010 |
| Received:December 02, 2019 |
| 基金项目:湖南省自然科学青年基金项目（2019JJ50463）；湖南省教育厅科研计划项目（2016C1237）；湖南省临床医疗技术创新引导项目（2018SK51201）；湖南省教育厅科研计划项目（2016C1237）；湖南省研究生科研创新项目（CX20190593）；湖南中医药大学中医学国内一流建设学科（2018－2020年）。 |
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| Effects of Mieyou Decoction on FoxO3a, Bim and FasL in Mouse Model of Helicobacter Pylori Associated Gastritis with Spleen-Stomach Damp-Heat Syndrome |
| XU Yin,ZHANG Yu,CHEN Mo,ZHOU Yifang,ZENG Rong,GUO Xuan,YU Bin,WANG Xiaojuan |
| (The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of Mieyou Decoction on FoxO3a, Bim and FasL in mice with helicobacter pylori (HP)-associated gastritis (HAG) of spleen-stomach damp-heat syndrome, and to explore its therapeutic mechanism on HAG of spleen-stomach damp-heat syndrome. Methods A total of 60 SPF-level BALB/c mice were randomly divided into a model group, a stomach quadruple group, a high-dose Mieyou Decoction group (Mieyou high group), and a low-dose Mieyou Decoction group (Mieyou low group), a middle-dose Mieyou Decoction group (Mieyou middle group), and a normal group, a total of 6 groups, with 10 mice in each group. The mouse model of HAG of spleen-stomach damp-heat syndrome was established by the composite cause (fat food + helicobacter pylori + damp heat environment) for the other 5 groups except the normal group. After successful modeling, the mice were given the corresponding intervention. The normal group and the model group were given distilled water by gavage, for consecutive 14 days. The apoptosis of gastric mucosa was detected by TUNEL method. The expressions and gene content of FoxO3a, FasL and Bim proteins in gastric mucosa were detected by Western blot and RT-PCR. Results The apoptosis rate of gastric mucosa was detected by TUNEL method. The apoptotic cells in the model group were significantly increased compared with the normal group. The apoptotic cells in the other treatment groups were significantly reduced compared with the model group, except the Mieyou low group. The expression of FoxO3a, FasL and Bim protein and mRNA in the model group was significantly increased than that in the normal group (P<0.01). Compared with the model group, the expression of FoxO3a, FasL and Bim protein and mRNA in the Mieyou high group, the Mieyou middle group and the stomach quadruple group were decreased when compared with the model group, and the difference was statistically significant (P<0.01). Conclusion The middle and high dose Mieyou Decoction can effectively treat HAG of spleen-stomach damp-heat syndrome. The mechanism may be that Mieyou Decoction can inhibit the apoptosis of gastric epithelial cells by regulating the expression of FoxO3a and down-regulating the content of apoptosis factors FasL and Bim. |
| Key words: HP associated gastritis Mieyou Decoction spleen-stomach damp-heat syndrome apoptosis FoxO3a FasL Bim |
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