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苏炳烛,简功辉,谢青玲,唐晓璐,杨卓,向黎黎,陆小龙,熊辉.膝骨关节炎肝肾亏虚证与寒湿痹阻证的血浆代谢组学差异研究[J].湖南中医药大学学报英文版,2020,40(6):749-753.[Click to copy
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| 膝骨关节炎肝肾亏虚证与寒湿痹阻证的血浆代谢组学差异研究 |
| 苏炳烛,简功辉,谢青玲,唐晓璐,杨卓,向黎黎,陆小龙,熊辉 |
| (湖南中医药大学, 湖南 长沙 410208;鞍山市第三医院, 辽宁 鞍山 114031;湖南中医药大学第二附属医院, 湖南 长沙 410005) |
| 摘要: |
| 目的 研究膝骨关节炎(knee osteoarthritis,KOA)肝肾亏虚证与寒湿痹阻证的差异代谢物，探讨KOA肝肾亏虚证与寒湿痹阻证辨证差异的物质代谢轮廓。方法 选取KOA肝肾亏虚证和寒湿痹阻证患者各30例，采用核磁共振氢谱(1H nuclear magnetic resonance spectroscopy，1H-NMR)技术测定血浆中的小分子代谢物，采用主成分分析法和正交偏最小二乘法-判别分析,进行多元统计分析。结果 肝肾亏虚组与寒湿痹阻组血浆样本有5个差异代谢物有统计学意义（P<0.05），其中高密度脂蛋白、柠檬酸、低密度脂蛋白/极低密度脂蛋白在肝肾亏虚组患者中含量明显高于寒湿痹阻组患者，而醋酸、组氨酸含量明显低于寒湿痹阻组患者。依富集程度（P<0.05）判别代谢通路为乙醛酸和二羟酸代谢、组氨酸代谢、柠檬酸循环、β-丙氨酸代谢、丙酮酸代谢、糖酵解和糖异生。结论 KOA肝肾亏虚证和寒湿痹阻证患者存在差异代谢物，分型的差异代谢物质基础可能是柠檬酸盐、组氨酸和醋酸。其中醋酸、组氨酸可能为寒湿痹阻证的标志性代谢产物。 |
| 关键词: 膝骨关节炎 肝肾亏虚 寒湿痹阻 核磁共振氢谱 代谢组学 |
| DOI：10.3969/j.issn.1674-070X.2020.06.021 |
| Received:May 20, 2020 |
| 基金项目:湖南省自然科学基金项目（2019JJ40224）。 |
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| Study on the Difference of Plasma Metabolomics Between Liver and Kidney Deficiency Syndrome and Cold-Dampness Bi Syndrome in Knee Osteoarthritis |
| SU Bingzhu,JIAN Gonghui,XIE Qingling,TANG Xiaolu,YANG Zhuo,XIANG Lili,LU Xiaolong,XIONG Hui |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Third Hospital of Anshan, Anshan, Liaoning 114031, China;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China) |
| Abstract: |
| Objective To study the difference of metabolites between liver-kidney deficiency syndrome and cold-dampness Bi syndrome in knee osteoarthritis (KOA), and to explore the material metabolism profile of the difference between liver-kidney deficiency syndrome and cold-dampness Bi syndrome in KOA. Methods The plasma of 30 KOA patients with liver and kidney deficiency syndrome and cold-dampness Bi syndrome were collected. 1H nuclear magnetic resonance spectroscopy (1H-NMR) technology was applied to determine small molecule metabolites in plasma. Principal component analysis and partial least squares discrimination analysis were performed to conduct multivariate statistical analysis. Results There were 5 different metabolites in the plasma samples of liver and kidney deficiency group and cold-dampness Bi group, with statistical significance (P<0.05). The contents of high density lipoprotein (HDL), citrate and low density lipoprotein (LDL)/very low density lipoprotein (VLDL) in patients with liver-kidney deficiency group were significantly higher than those in patients with cold-dampness Bi group, while the contents of acetate and histidine were significantly lower than those in patients with cold-dampness Bi group. According to the enrichment degree (P<0.05), the metabolic pathway was identified as glyoxylate and dicarboxylate metabolism, histidine metabolism, citrate cycle, β-alanine, pyruvate metabolism, glycolysis/gluconeogenesis. Conclusion There are different metabolites in KOA patients with liver and kidney deficiency syndrome and cold-dampness Bi syndrome. Citrate, histidine and acetate may be the different metabolites of syndrome type. Acetate and histidine may be the iconic metabolites of cold-dampness Bi syndrome. |
| Key words: knee osteoarthritis deficiency of liver and kidney cold-dampness Bi syndrome 1H nuclear magnetic resonance spectroscopy metabolomics |
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