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黄钲凯,梁明坤,吴发胜,陆飞国,陈广文,梁健.基于网络药理学的左金丸治疗胃癌作用机制研究[J].湖南中医药大学学报英文版,2020,40(6):709-716.[Click to copy
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| 基于网络药理学的左金丸治疗胃癌作用机制研究 |
| 黄钲凯,梁明坤,吴发胜,陆飞国,陈广文,梁健 |
| (湖南中医药大学中西医结合学院, 湖南 长沙 410208;广西中医药大学附属瑞康医院, 广西 南宁 530001;广西中医药大学第一附属医院, 广西 南宁 530023;湖南中医药大学中西医结合学院, 湖南 长沙 410208;广西大学医学院, 广西 南宁 530004) |
| 摘要: |
| 目的 基于网络药理学方法探讨左金丸治疗胃癌的作用机制。方法 利用TCMSP数据库获得左金丸所含中药的化学成分及对应作用靶点基因，检索GeneCards和OMIM数据库获得疾病胃癌相关靶点基因，然后取两者的交集,得到左金丸作用于胃癌的预测靶基因；利用Cytoscape 3.7.1软件构建左金丸化合物-作用靶点-疾病三者相互关系网络，采用STRING数据库对左金丸作用于胃癌的预测靶基因进行蛋白互作网络分析，利用Cytoscape3.7.1软件的插件cytohubba筛选出关键靶基因，并利用ONCOMINE数据库分析关键靶基因在胃癌中的表达情况；利用在线工具DAVID进行GO功能富集分析并利用Cytoscape3.7.1软件的ClueGO插件进行KEGG通路富集分析。结果 共筛选出左金丸作用于胃癌的预测靶基因68个,关键靶基因15个，这些关键基因在ONCOMINE数据库的胃癌样本和正常样本中的表达存在显著性差异。GO功能富集分析显示，左金丸作用于胃癌的预测靶基因主要涉及对有机物质的反应、细胞凋亡的负调控、细胞程序性死亡的负调控等生物过程；KEGG通路富集分析主要涉及癌症通路、p53信号通路、VEGF信号通路等。结论 本研究初步揭示了左金丸治疗胃癌的主要作用靶点及多靶点、多维调控、协同作用、整体调节的作用机制，可为后续进一步深入研究其作用机制提供参考。 |
| 关键词: 左金丸 胃癌 网络药理学 信号通路 |
| DOI：10.3969/j.issn.1674-070X.2020.06.014 |
| Received:June 02, 2019 |
| 基金项目:国家自然科学基金项目（81660743）；广西自然科学基金项目（2018JJA140950）。 |
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| Mechanism of Zuojin Pills in the Treatment of Gastric Cancer Based on Network Pharmacology |
| HUANG Zhengkai,LIANG Mingkun,WU Fasheng,LU Feiguo,CHEN Guangwen,LIANG Jian |
| (College of Integrated Chinese Medicine and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, China;The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, Guangxi 530023, China;College of Integrated Chinese Medicine and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Medical College of Guangxi University, Nanning, Guangxi 530004, China) |
| Abstract: |
| Objective To study the mechanism of Zuojin Pills in the treatment of gastric cancer based on network pharmacology. Methods Chemical constituents and corresponding action target genes of Chinese materia medica in Zuojin Pills were obtained from TCMSP database, while GeneCards and OMIM database was retrieved for the target genes of gastric cancer. Then the intersection of the 2 groups of genes were taken to get the predicted target gene of Zuojin Pills acting on gastric cancer. Cytohubba, the plug-in of Cytoscape3.7.1 software was used to screen out key target genes. And the ONCOMINE database was used to analyze the expression of key target genes in gastric cancer. Gene ontology (GO) enrichment analysis was conducted by DAVID online tool and kyoto encyclopedia of genes and genomes (KEGG) pathway analysis was carried out using Cytoscape3.7.1 software. Results A total of 68 target genes and 15 key genes were screened for Zuojin Pills in the treatment of gastric cancer. There were significant differences in the expression of these key genes in gastric cancer samples and normal samples in ONCOMINE database. GO functional enrichment analysis showed that Zuojin Pills acting the predicted target gens of gastric cancer involved in a series of biological process, such as response to organic substance, negative regulation of apoptosis, negative regulation of programmed cell death, etc. KEGG pathway enrichment analysis mainly involved pathways in cancer, p53 signaling pathway, VEGF signaling pathway, etc. Conclusion This study preliminarily revealed the main targets of Zuojin Pills in the treatment of gastric cancer, and the action mechanism of multi-target, multi-dimensional regulation, synergistic effect and overall regulation, which can provide beneficial thinking for further deeper study on its action mechanism in the furture. |
| Key words: Zuojin Pills gastric cancer network pharmacology signaling pathway |
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