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林燊,齐诗仪,章思佳,林栋.不同时长电针对小鼠大脑皮质区BDNF及CREB蛋白表达的影响[J].湖南中医药大学学报英文版,2020,40(3):333-336.[Click to copy
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| 不同时长电针对小鼠大脑皮质区BDNF及CREB蛋白表达的影响 |
| 林燊,齐诗仪,章思佳,林栋 |
| (福建中医药大学, 福建 福州 350122) |
| 摘要: |
| 目的 观察不同干预时长电针对正常小鼠大脑皮质区环磷腺苷效应元件结合蛋白（cAMP-response element binding protein，CREB）及脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）蛋白表达的影响。方法 采用随机数字表法将36只正常小鼠分为空白组、手针组、电针5 s组、电针10 s组、电针20 s组、电针60 s组，每组6只。空白组仅模拟抓取过程；手针组针刺小鼠后海穴后立即取针不留针；电针组取后海穴透皮后分别向左右两侧沿皮针刺，针柄连接电针仪，按组别分别刺激不同时长（5 s、10 s、20 s、60 s），反复15次，频率2 Hz，强度2 mA，连续波。连续干预7 d后，采用Western Blot法检测各组小鼠大脑皮质区BDNF及CREB蛋白的表达。结果 电针5 s组及电针10 s组大脑皮质区CREB表达较空白组差异无统计学意义（P>0.05）；电针20 s组及电针60 s组CREB表达较空白组及手针组差异有显著统计学意义（P<0.001）；电针20 s组CREB表达升高较电针5 s、10 s组差异有显著统计学意义（P<0.001）；电针60 s组较电针20 s组差异有统计学意义（P<0.05），且较其余各组差异均有显著统计学意义（P<0.001）。大脑皮质区BDNF表达各组间差异无统计学意义（P>0.05）。结论 小鼠大脑皮质区CREB表达变化在电针20 s组（累积刺激5 min）开始起效，而电针5 s组、电针10 s组刺激无法触发大脑皮质区响应蛋白的表达，初步推断电针20 s组为针刺响应时间。 |
| 关键词: 大脑皮质区 电针 干预时长 环磷腺苷效应元件结合蛋白 脑源性神经营养因子 |
| DOI：10.3969/j.issn.1674-070X.2020.03.017 |
| Received:September 05, 2019 |
| 基金项目:福建省自然科学基金项目（2017J01540）。 |
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| Effects of Different Electroacupuncture Durations on the Expression of BDNF and CREB Protein in Mice Cortex |
| LIN Shen,QI Shiyi,ZHANG Sijia,LIN Dong |
| (Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China) |
| Abstract: |
| Objective To observe the effects of different intervention durations on the expression of brain-derived neurotrophic factor (BDNF) and cAMP-response element binding protein (CREB) in the cortex of normal mice. Methods A total of 36 normal mice were divided into a blank group, a hand acupuncture group, an electroacupuncture 5 s group, an electroacupuncture 10 s group, an electroacupuncture 20 s group and an electroacupuncture 60 s group with random number table method, with 6 rats in each group. The blank group only simulated the grasping process. The hand acupuncture group immediately acupunctured Houhai acupoint of the mice and then withdrew the needle without retaining the needle. The electroacupuncture groups acupunctured Houhai acupoint transdermally, and then acupunctured to the left and right side respectively. The needle handle was connected with the electroacupuncture instrument. The groups were stimulated for 5 s, 10 s, 20 s, 60 s, repeated 15 times, frequency 2 Hz, intensity 2 mA, with continuous wave. After continuous intervention for 7 days, Western Blot method was used to detect the expression of BDNF and CREB protein in the cerebral cortex of mice in each group. Results There was no significant difference in the expression of CREB between the electroacupuncture 5 s group and the electroacupuncture 10 s group (P>0.05). The expression of CREB in electroacupuncture 20 s group and the electroacupuncture 60 s group was significantly different than that in the blank group and the hand acupuncture group (P<0.001). The increase of expression of CREB in the electroacupuncture 20 s group was significantly different than that of the electroacupuncture 5 s group and the electroacupuncture 10 s group (P<0.001). The difference between the electroacupuncture 60 s group and the electroacupuncture 20 s group was statistically significant (P<0.05), and the difference was statistically significant compared with the other groups (P<0.001). There was no significant difference in the expression of BDNF in the cerebral cortex between groups (P>0.05). Conclusion The change of CREB expression in the cerebral cortex of mice began to take effect in the electroacupuncture 20 s group (cumulative stimulation for 5 min), but the stimulation of the electroacupuncture 5 s and 10 s groups could not trigger the expression of response proteins in the cerebral cortex. The preliminary conclusion is that the electroacupuncture 20 s group is the acupuncture response time. |
| Key words: cerebral cortex electroacupuncture duration of intervention cAMP-response element binding protein brain-derived neurotrophic factor |
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