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徐拥建,冯高飞,张云城,黄裕华,邓远军.参苓白术散对非酒精性脂肪性肝病大鼠肝细胞、Kupffer细胞5-LO/LTB4通路的影响[J].湖南中医药大学学报英文版,2020,40(3):292-297.[Click to copy
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| 参苓白术散对非酒精性脂肪性肝病大鼠肝细胞、Kupffer细胞5-LO/LTB4通路的影响 |
| 徐拥建,冯高飞,张云城,黄裕华,邓远军 |
| (北京中医药大学深圳医院(龙岗), 广东 深圳 518172;暨南大学中医药学院, 广东 广州 510632) |
| 摘要: |
| 目的 基于5-脂氧化酶（5-lipoxygenase，5-LO）/白三稀B4（leukotrienes B4，LTB4）通路探讨参苓白术散对非酒精性脂肪性肝病（nonalcoholic fatty liver disease，NAFLD）大鼠肝细胞、库普弗（Kupffer）细胞作用机制。方法 通过Ⅳ型胶原酶离体循环灌注法分离SD大鼠肝细胞及Kupffer细胞，利用油红O染色观察肝组织脂滴蓄积情况；ELISA法检测肝组织LTB4含量；RT-PCR法、Western blot法检测大鼠肝细胞及Kupffer细胞5-LO、白三烯B4受体（LTB4 receptor type 1，BLT1）mRNA及蛋白表达水平。结果 高脂饮食8周能诱导大鼠NAFLD表现，建模成功。与模型组比较，2个药物剂量干预组大鼠肝组织LTB4含量均显著下降（P<0.01）；肝细胞、Kupffer细胞中的5-LO、BLT1 mRNA及其蛋白表达水平均亦有不同程度的下调（P<0.01）。2个剂量组间比较，以高剂量参苓白术散组效果较为显著（P<0.05或P<0.01）。结论 参苓白术散可能通过抑制NAFLD大鼠肝细胞、Kupffer细胞5-LO/LTB4通路的激活，减轻肝脏脂质蓄积，发挥防治NAFLD的作用。 |
| 关键词: 参苓白术散 肝细胞 Kupffer细胞 5-脂氧化酶 白三稀B4 |
| DOI：10.3969/j.issn.1674-070X.2020.03.009 |
| Received:September 29, 2019 |
| 基金项目:北京中医药大学校级科研项目（2018BUCMXJKY013）；深圳市龙岗区医疗卫生科技计划资助项目（20170405190544730）。 |
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| Effects of Shenling Baizhu Powder on 5-LO/ LTB4 Pathway in Hepatocytes and Kupffer Cells of Nonalcoholic Fatty Liver Disease Rats |
| XU Yongjian,FENG Gaofei,ZHANG Yuncheng,HUANG Yuhua,DENG Yuanjun |
| (Shenzhen Hospital (Longgang), Beijing University of Chinese Medicine, Shenzhen, Guangdong 518172, China;School of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510632, China) |
| Abstract: |
| Objective To investigate the action mechanism of Shenqi Baizhu Powder (SBP) on hepatocytes and Kupffer cells of nonalcoholic fatty liver disease (NAFLD) rats based on 5-lipoxygenase (5-LO)/leukotrienes B4 (LTB4) pathways. Methods SD rat hepatocytes and Kupffer cells were isolated by in vitro perfusion of type IV collagenase. The hepatic lipid accumulation of liver tissue was detected by oil red O staining. The content of LTB4 in liver tissue were detected by enzyme-linked immunosorbent ELISA. The mRNA and proteins expression of 5-LO and LTB4 receptor type 1 (BLT1) in were detected by RT-PCR and Western blot. Results It was observed that the rats of NAFLD model were induced by high-fat-diet with 8 weeks successfully. Compared with the model group, the content of LTB4 were decreased significantly in in the two drug intervention groups (P<0.01), as well as the lower expression levels of gene and proteins expression of 5-LO and BLT1 in hepatocytes and Kupffer cells for the varying degrees (P<0.01). Compared between two dose groups, the high-dose SBP group showed more significant effects (P<0.05 or P<0.01). Conclusion SBP may play a role in preventing and treating NAFLD by inhibiting the activation of 5-LO/LTB4 pathway in liver cells and Kupffer cells of NAFLD rats, and reducing liver lipid accumulation. |
| Key words: Shenling Baizhu Powder hepatocyte Kupffer cells 5-LO LTB4 |
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