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艾珏萍,罗婷,吴安林,黄晓蒂,杨程,叶平,谢秀惠,彭亮.小鱼际滚法对组织机化期骨骼肌钝性损伤家兔Fibronectin-1与CTGF-1表达的影响[J].湖南中医药大学学报英文版,2020,40(2):204-208.[Click to copy
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| 小鱼际滚法对组织机化期骨骼肌钝性损伤家兔Fibronectin-1与CTGF-1表达的影响 |
| 艾珏萍,罗婷,吴安林,黄晓蒂,杨程,叶平,谢秀惠,彭亮 |
| (湖南中医药大学针灸推拿学院, 湖南 长沙 410208;长沙医学院, 湖南 长沙 410219;湖南中医药大学中医学院医学基础教学实验中心, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察小鱼际滚法对组织机化期骨骼肌钝性损伤家兔纤连蛋白-1（fibronectin-1，FN-1）、结缔组织生长因子-1（connective tissue growth factor，CTGF-1）表达的影响。方法 选取30只成年新西兰大耳白兔，随机均分为手法治疗组、模型对照组、空白对照组，各10只。手法治疗组予造模、治疗处理，模型对照组予造模、不治疗处理，空白对照组予不造模、不治疗处理，采取自制重力铅锤击打兔右后下肢股四头肌造成骨骼肌钝性损伤模型，并在疗程3 d后3组全处死，在兔股四头肌受损区域内取肌肉组织，HE染色观察组织形态的变化，Western-Blot检测治疗前后FN-1、CTGF-1的变化情况。结果 HE染色结果显示：模型对照组与空白对照组对比，其模型对照组肌纤维受损较多，并有不规则结缔样组织填充其中；手法治疗组与模型对照组对比，手法治疗组新生的肌纤维及新生血管更明显，炎性细胞较少，肌肉形态受损较轻。Western-Blot结果显示：模型对照组FN-1、CTGF-1指标显著高于空白对照组，手法治疗组FN-1、CTGF-1指标均显著高于空白对照组、低于模型对照组，差异均有统计学意义（P<0.05）。结论 在组织机化期骨骼肌钝性损伤模型家兔股四头肌修复过程中，运用小鱼际滚法治疗，可有助修复受损的骨骼肌，使炎性细胞减少，促进骨骼肌新生，改善家兔运动功能，提高疗效，值得推广应用。 |
| 关键词: 骨骼肌 组织机化期 钝性损伤 小鱼际滚法 纤连蛋白-1 结缔组织生长因子-1 |
| DOI：10.3969/j.issn.1674-070X.2020.02.017 |
| Received:September 01, 2019 |
| 基金项目:湖南省自然科学基金（2018JJ2292）；湖南省教育科学“十三五”规划2017年度课题（编号85）。 |
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| The Effects of Interthenar Rolling Method on Rabbit Fibronectin-1, Ctgf-1 Induced By Tissue Mechanization of Skeletal Muscle Injury |
| AI Jueping,LUO Ting,WU Anlin,HUANG Xiaodi,YANG Cheng,YE Ping,XIE Xiuhui,PENG Liang |
| (College of Acupuncture and Moxibustion, Hunan University of CM, Changsha, Hunan 410208, China;Changsha Medical University, Changsha, Hunan 410219, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of Interthenar rolling method on the expression of skeletal muscle blunt injury fibronectin-1 (Fibronecttin-1, FN-1) and connective tissue growth factor (CTGF-1) in rabbits with blunt injury of skeletal muscle in tissue mechanization stage. Methods A total of 30 adult New Zealand rabbits with big ears were randomly divided into a manual treatment group, a model control group and a blank control group, with 10 cases in each group. The manipulation treatment group was given model making and treatment, and the model control group was given modeling without treatment. The blank control group do not make model and treatment. A blunt injury model of skeletal muscle was made by hitting the rabbit's right lower limb quadriceps with a self-made homemade gravity lead hammer, and all the groups were sacrificed after 3 days of treatment. Muscle tissue was taken from the rabbit quadriceps damaged area, and HE staining was used for observing tissue morphological changes. The changes of Fibronectin-1 and CTGF-1 before and after treatment were detected by Western-Blot. Result The results of HE showed that compared with the model control group and the blank control group, the muscle fibers in the model control group were more damaged and serious than those in the manipulation treatment group, in which irregular connective tissue was filled. Compared with the manipulation treatment and model control group, the new muscle fibers and neovascularization in the manipulation treatment group were more obvious, and the inflammatory cells were fewer than those in the model control group. The results of Western-Blot showed that the indexes of FN-1 and CTGF-1 in the manipulation treatment group were significantly higher than those in the blank control group.The indexes of FN-1 and CTGF-1 in the manual treatment group were significantly higher than those of the blank control group and lower than those in the model control group, and the differences were statistically significant (P<0.05). Conclusion In the process of repairing quadriceps femoris in rabbits with blunt injury of skeletal muscle in organized phase, the method of interthenar rolling was used, which can help to repair the damaged skeletal muscle, to reduce inflammatory cells, to promote skeletal muscle regeneration, to improve the motor function of rabbits, and improve the curative effects. It is worthy of popularizing and applying. |
| Key words: skeletal muscle tissue mechanization period blunt injury interthenar rolling method fibronectin-1 connective tissue growth factor-1 |
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