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周海亮,周兴,李波男,周青,何清湖.雄蚕益肾方对肾阳虚大鼠睾丸抗氧化作用及睾丸Bcl-2、Cyt-c蛋白表达的影响[J].湖南中医药大学学报英文版,2019,39(12):1435-1439.[Click to copy
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| 雄蚕益肾方对肾阳虚大鼠睾丸抗氧化作用及睾丸Bcl-2、Cyt-c蛋白表达的影响 |
| 周海亮,周兴,李波男,周青,何清湖 |
| (湖南中医药大学中西结合学院, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 研究雄蚕益肾方对肾阳虚大鼠抗氧化作用及睾丸Bcl-2、Cyt-c蛋白表达的影响。方法 取雄性SD大鼠40只，随机分成正常组10只、造模组30只。造模组予200 mg/kg腺嘌呤混悬液造肾阳虚模型成功后，随机分为模型组、左卡尼汀组、雄蚕益肾组，正常组与模型组给予生理盐水灌胃，其他组分别给予相应药物连续灌胃，4 mL/d。4周后检测睾丸组织SOD、MDA、GSH-Px及血清性激素相关指标，免疫组化法测定睾丸Bcl-2、Cyt-c蛋白表达情况，取两侧睾丸做常规病理切片分析。结果 与模型组相比，雄蚕益肾方组睾丸GSH-PX、SOD、血清T、E2浓度显著升高（P<0.01），睾丸MDA浓度明显降低（P<0.05），雄蚕益肾方组睾丸Bcl-2蛋白阳性表达率明显升高（P<0.01），Cyt-c蛋白阳性表达率明显降低（P<0.01）；与模型组相比，雄蚕益肾方组睾丸生精上皮尚比较完整，组织内生精小管内可见精母细胞，生精小管内仍有大量精子生成，管腔可见大量精子。结论 雄蚕益肾方能提高肾阳虚大鼠睾丸组织抗氧化作用，调节生精细胞凋亡，这可能是其调节生精功能的机制之一。 |
| 关键词: 雄蚕益肾方 肾阳虚 抗氧化作用 生精功能 |
| DOI：10.3969/j.issn.1674-070X.2019.12.003 |
| Received:May 27, 2019 |
| 基金项目:国家自然科学基金资助项目（81673984，81774324）；湖南省自然科学基金资助（2018JJ3402）；湖南省教育厅优秀青年项目（16B200）；长沙市杰出创新青年人才项目（kq1802015）；湖南省高层次卫生人才225工程医学学科骨干人才培养对象（湘卫函[2019]196号）；湖南创新型省份建设专项经费资助“湖湘青年英才”支持计划（2019RS2041）。 |
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| Effects of Xiongcan Yishen Formula on Testicular Antioxidant Activity and Expression of Testicular Bcl-2 and Cyt-c Protein in Rats with Kidney-Yang Deficiency |
| ZHOU Hailiang,ZHOU Xing,LI Bonan,ZHOU Qing,HE Qinghu |
| (School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To study the effects of Xiongcan Yishen formula on antioxidant effect and expression of testicular bcl-2 and Cyt-c protein in rats with kidney-Yang deficiency. Methods A total of 40 male SD rats were randomly divided into a normal group (n=10) and a model group (n=30). After successful modeling of kidney-Yang deficiency model by giving 200 mg/kg adenine suspension to the model group, and they were randomly divided into the model group, levocarnitine group and Xiongcan Yishen formula group. The normal group and the model group were given normal saline by gavage, and the other groups were given continuous gavage with corresponding drugs, 4 mL/d. After 4 weeks, SOD, MDA, gsh-px and serum sex hormone related indicators in testicular tissue were detected, and the expressions of Bcl-2 and Cyt-c proteins in testicle were determined by immunohistochemistry. Routine pathological sections of both sides of testicle were taken for analysis. Results Compared with the model group, GSH-Px, SOD, serum T and E2 concentrations were significantly increased in the Xiongcan Yishen formula group (P<0.01), and MDA concentrations were significantly decreased (P<0.05). The positive expressions of Bcl-2 proteins were significantly increased (P<0.01), and the positive expression rate of Cyt-c protein was significantly reduced in the Xiongcan Yishen formula group (P<0.01). Compared with the model group, the spermatogenic epithelium of the testis in the Xiongcan Yishen formula group was relatively complete. Spermatogonial cells could be seen in the spermatogonial tubules in the tissue, and there were still a lot of spermatogenesis in the spermatogonial tubules, and a lot of spermatozoa could be seen in the lumen. Conclusion Xiongcan Yishen formula can improve the antioxidant effect of the testicular tissue and regulate the apoptosis of spermatogenic cells in rats with kidney-Yang deficiency, which may be one of the mechanisms of regulating spermatogenic function. |
| Key words: Xiongcan Yishen Formula Kidney-Yang deficiency antioxidation sperm production function |
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