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廖宗力,孙璐,朱重政,黄文韬,谭静,阳仁达.不同促透剂运用于隔药饼灸对高脂血症合并动脉粥样硬化兔主动脉形态及血清IL-6、IL-10的影响[J].湖南中医药大学学报英文版,2019,39(11):1368-1372.[Click to copy
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| 不同促透剂运用于隔药饼灸对高脂血症合并动脉粥样硬化兔主动脉形态及血清IL-6、IL-10的影响 |
| 廖宗力,孙璐,朱重政,黄文韬,谭静,阳仁达 |
| (湖南中医药大学, 湖南 长沙 410208;惠州市中医院, 广东 惠州 516000;湖南中医药大学第二附属医院, 湖南 长沙 410005) |
| 摘要: |
| 目的 探讨氮酮和冰片作为促透剂运用于隔药饼灸对高脂血症合并动脉粥样硬化兔主动脉病理形态及血清炎症因子的影响。方法 选取40只新西兰纯种兔随机分成5组，每组8只，即正常组、模型组、无促透剂组、氮酮组和冰片组。高脂血症合并动脉粥样硬化兔模型造模成功后除正常组和模型组外，其余各组采用隔药饼灸干预，每日1次，连续4周。采用ELISA检测血清白细胞介素6（Interleukin 6，IL-6）、白细胞介素10（Interleukin 10，IL-10）含量；采用HE染色，光学显微镜下观察主动脉组织形态。结果 与正常组比，模型组IL-6水平显著升高、IL-10水平显著降低（P<0.01）；与模型组比，无促透剂组、氮酮组及冰片组IL-6水平显著降低（P<0.01）、IL-10水平升高（P<0.05）；与无促透剂比，氮酮组和冰片组IL-6水平显著降低（P<0.01）、IL-10差异无统计学意义（P>0.05）；与氮酮组比，冰片组IL-6水平显著降低（P<0.01）、IL-10差异无统计学意义（P>0.05）。主动脉组织镜检结果示：正常组无异常；模型组可见组织整体凌乱，管腔壁可见明显增厚，内皮坏死，有明显的脂质斑块及泡沫细胞；无促透剂组有内膜增厚，可见少量泡沫细胞，细胞排列较模型组稍好，脂质沉积明显较模型组减少，斑块不明显；氮酮组和冰片组无显著异常。结论 隔药饼灸对高脂血症合并动脉粥样硬化兔具有抑制炎症反应和抗动脉粥样硬化作用，在药饼中加入促透剂后施灸疗效更优，可能与药饼中药物透皮吸收增强有关，值得进一步临床应用和研究。 |
| 关键词: 隔药饼灸 促透剂 高脂血症 动脉粥样硬化 白细胞介素6 白细胞介素10 |
| DOI：10.3969/j.issn.1674-070X.2019.11.017 |
| Received:July 05, 2019 |
| 基金项目:湖南省高校创新平台开放基金项目（16K068）；2017年湖南省大学生创新实验研究项目（CX2017B431）；湖南省大学生创新实验项目（017-132）。 |
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| Effects of Different Transdermal Enhancers for Herbal Cake-separated Moxibustion on the Aortic Morphology and Serum Il-6 and IL-10 Levels in Rabbits with Hyperlipidemia Concomitant with Atherosclerosis |
| LIAO Zongli,SUN Lu,ZHU Zhongzheng,HUANG Wentao,TAN Jing,YANG Renda |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Huizhou Hospital of Traditional Chinese Medicine, Huizhou, Guangdong 516000, China;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410000, China) |
| Abstract: |
| Objective To explore the effects of using laurocapram and borneol as transdermal enhancers for herbal cake-separated moxibustion on the arterial pathological morphology and serum inflammatory factors in rabbits with hyperlipidemia concomitant with atherosclerosis. Methods A total of 40 New Zealand purebred rabbits were randomized into 5 groups including a normal group, a model group, a transdermal enhancer-free group, a laurocapram group and a borneol group, with 8 cases in each group. Rabbit models with hyperlipidemia concomitant with atherosclerosis were established, after which except the normal group and the model group, the other groups were given herbal cake-separated moxibustion intervention 1 time each day for consecutive 4 weeks. Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum Interleukin 6 (IL-6) and Interleukin 10 (IL-10) levels. HE staining was performed to observe the aortic tissue morphology under optical microscope. Results Compared with normal group, model group had significantly increased IL-6 level and significantly decreased IL-10 level (P<0.01). Compared with model group, transdermal enhancer-free group, laurocapram group and borneol group had a markedly decreased IL-6 level (P<0.01) and increased IL-10 level (P<0.05). Compared with transdermal enhancer-free group, laurocapram group and borneol group had significantly decreased IL-6 level (P<0.01), while the differences of IL-10 level were not statistically significant (P>0.05). Compared with laurocapram group, borneol group had significantly decreased IL-6 level (P<0.01), while the differences of IL-10 level were not statistically significant (P>0.05). The aortic tissue microscopy results showed that in the normal group, no abnormality was noted; in the model group, the tissues were in disorder in the whole, the luminal wall was obviously thickened, the endothelium was necrotic, and notable lipid plaques and foam cells were noted; in transdermal enhancer-free group, the endarterium was thickened, a small amount of foam cells were noted, the cells were arranged slightly better than those in model group, the lipidosis decreased notably than that in model group, and the plaques were not obvious; no obvious abnormality was noted in neither Laurocapram group nor borneol group. Conclusion Herbal cake-separated moxibustion can inhibit the inflammatory reactions and promote the anti-atherosclerotic effect in rabbits with hyperlipidemia concomitant with atherosclerosis, whose efficacy is more superior when transdermal enhancers are added in the herbal cake, which may be resulted by the enhancement of the transdermal absorbability of the drugs in the herbal cake. It is worth to be further clinically applied and studied. |
| Key words: herbal cake-separated moxibustion transdermal enhancer hyperlipidemia atherosclerosis interleukin-6 interleukin-10 |
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