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蔺晓源,徐菲,张嘉伦,赵应松,王敏,刘杰民,胡国恒.健脾益肠散对溃疡性结肠炎大鼠CD86和ICAM-1表达的影响[J].湖南中医药大学学报英文版,2019,39(11):1321-1325.[Click to copy
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| 健脾益肠散对溃疡性结肠炎大鼠CD86和ICAM-1表达的影响 |
| 蔺晓源,徐菲,张嘉伦,赵应松,王敏,刘杰民,胡国恒 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学中医学国内一流建设学科, 湖南 长沙 410208;贵阳中医学院, 贵州 贵阳 550002;贵州省人民医院, 贵州 贵阳 550002) |
| 摘要: |
| 目的 观察健脾益肠散对溃疡性结肠炎（ulcerative colitis，UC）大鼠CD86分子（CD86）和细胞间黏附分子-1（intercellular adhesion molecule-1，ICAM-1）表达的影响，探讨其对UC肠黏膜的免疫保护机制。方法 将60只SD大鼠先随机分为空白组和造模组，造模组采用2，4，6-三硝基苯磺酸（2，4，6-trinitrobenzene sulfonic acid，TNBS）溶液灌肠法制备UC大鼠模型，待模型复制成功后再将造模组随机分为模型组、西药组和中药高、中、低剂量组；干预21 d后，免疫组化法检测各组大鼠结肠组织中CD86的阳性表达，Western Blot、RT-PCR法分别检测结肠中ICAM-1的蛋白和mRNA表达。结果 与空白组比较，模型组大鼠结肠组织中CD86的阳性表达以及ICAM-1的蛋白和mRNA表达均显著增高（P<0.01）；与模型组比较，中药高、中、低剂量组和西药组CD86、ICAM-1的表达均不同程度地降低（P<0.05或P<0.01），且以中药高剂量组最为明显（P<0.05或P<0.01）。结论 健脾益肠散可能通过降低结肠组织中CD86、ICAM-1的表达水平起到对UC结肠黏膜的免疫保护作用。 |
| 关键词: 溃疡性结肠炎 健脾益肠散 CD86分子 细胞间黏附分子-1 |
| DOI：10.3969/j.issn.1674-070X.2019.11.006 |
| Received:December 04, 2018 |
| 基金项目:国家自然科学基金项目（81560773）。 |
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| Effects of Jianpi Yichang Powder on Expression of CD86 Molecule, Intercellular Adhesion Molecule-1 in Rats with Ulcerative Colitis |
| LIN Xiaoyuan,XU Fei,ZHANG Jialun,ZHAO Yingsong,WANG Min,LIU Jiemin,HU Guoheng |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;The Domestic First Class Construction Discipline of Chinese Medicine in Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550002, China;People's Hospital of Guizhou Province, Guiyang, Guizhou 550002, China) |
| Abstract: |
| Objective To observe the effects of Jianpi Yichang Powder (JYP) on the expressions of CD86 molecule (CD86) and intercellular adhesion molecule-1 (ICAM-1) in ulcerative colitis (UC) rats, and to explore its immunoprotective mechanism of UC intestinal mucosa. Methods A total of 60 SD rats were randomly divided into a blank group and a modeling group. The UC models were established by the method of 2,4,6-trinitrobenzene sulfonic acid (TNBS) solution clyster. Then the successfully modeled rats were randomly divided into a model group, a western medicine group, a high, middle, and low dosage groups of Chinese herbal medicine. After intragastric administration for 21 days, immunohistochemical method was used to detect the positive expression of CD86. Western Blot and Real-Time PCR methods were used to detect the expression of ICAM-1 protein and mRNA in colonic tissue of rats in each group. Results The positive expression of CD86 and the protein and mRNA expressions of ICAM-1 in colonic tissue of the model group were significantly increased than those in the blank group (P<0.01). The expressions of CD86 and ICAM-1 in high, middle, low dose groups of Chinese herbal medicine groups and western medicine groups were decreased than the model group in different degrees (P<0.05 or P<0.01), and the high dose group of Chinese herbal medicine was the most obvious (P<0.05 or P<0.01). Conclusion JYP may play an immune protective role on colon mucosa of UC by decreasing the expression of CD86, ICAM-1 in colon tissue. |
| Key words: ulcerative colitis Jianpi Yichang Powder CD86 molecule intercellular adhesion molecule-1 |
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