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刘朝圣,申梦洁,韩晓凤,彭丽丽,刁庆春.红景天苷对UVB辐射HaCat细胞活性及Caspase-3/9蛋白表达的影响[J].湖南中医药大学学报英文版,2019,39(8):952-956.[Click to copy
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| 红景天苷对UVB辐射HaCat细胞活性及Caspase-3/9蛋白表达的影响 |
| 刘朝圣,申梦洁,韩晓凤,彭丽丽,刁庆春 |
| (湖南中医药大学第一附属医院皮肤科, 湖南 长沙 410007;重庆市第一人民医院皮肤科, 重庆 400011;漳州正兴医院医疗美容科, 福建 漳州 363000;湖南中医药大学护理学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 研究红景天苷对中波紫外线（ultraviolet B， UVB）辐射HaCat细胞增殖及Caspase-3、Caspase-9蛋白表达的作用。方法 将HaCat细胞分为6组：即正常对照组（Ⅰ组）、红景天苷中剂量组（Ⅱ组）、UVB辐射组（Ⅲ组）、UVB+红景天苷低剂量组（Ⅳ组）、UVB+红景天苷中剂量组（Ⅴ组）、UVB+红景天苷高剂量组（Ⅵ组），Ⅰ组未经任何处理，Ⅱ组加入40 μg/mL的红景天苷，Ⅲ组接受200 mJ/cm2剂量紫外线照射，Ⅳ、Ⅴ、Ⅵ组分别加入10、40、80 μg/mL红景天苷并进行200 mJ/cm2紫外线照射。HaCat细胞培养24 h后，采用MTT法检测细胞活力，流式细胞仪观察细胞周期，Western blotting法检测Caspase-3、Caspase-9表达的蛋白水平。结果 MTT结果显示随着红景天苷浓度的增加，HaCat细胞的生存率逐渐增加；流式细胞仪检测细胞周期显示HaCat细胞的S峰值随着红景天苷浓度的增加呈现上升趋势，Ⅵ组S峰值高于Ⅱ组；Western blotting显示Ⅵ组Caspase-3/9的蛋白表达量均有下降。结论 红景天苷可通过抑制Caspase-3和Caspase-9蛋白表达，减少UVB诱导HaCat细胞凋亡。 |
| 关键词: 红景天苷 中波紫外线 细胞增殖 Caspase-3 Caspase-9 |
| DOI：10.3969/j.issn.1674-070X.2019.08.005 |
| Received:June 15, 2018 |
| 基金项目:湖南省中医药科研计划重点项目（201518）；重庆市人社局博士后特别资助基金项目(Xm2015087)；湖南省“十二五”重点学科开放基金项目（ZXLCXK201508）。 |
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| Effect of Salidroside on Activity of HaCat Cell by UVB Radiation and Expression of Caspase-3/9 Protein |
| LIU Chaosheng,SHEN Mengjie,HAN Xiaofeng,PENG Lili,DIAO Qingchun |
| (Department of Dermatolgy, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Departmentof Dermatolgy, Chongqing First People's Hospital, Chongqing 400010, China;Department of Medical Cosmetology, The Zhangzhou Zhengxing Hospital, Zhangzhou, Fujian 363000, China;Nursing School, The Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the effects of Salidroside on proliferation and expression of Caspase-3/9 protein in HaCat cells induced by ultraviolet B (UVB) radiation. Methods HaCat cells were divided into 6 groups:a normal control group (group I), a medium dose of Salidroside group (group II), a UVB radiation group (group III), a UVB+ low dose of Salidroside group (group IV), a UVB+ medium dose group (group V), a UVB+ high dose of Salidroside group (VI group). Group I was not treated. Group II was added with 40 μg/mL of Salidroside; Group III was irradiated with ultraviolet radiation at 200 mJ/cm2; Group IV, Group V, Group VI were added with 10 μg/mL, 40 μg/mL, 80 μg/mL of Salidroside respectively and irradiated with 200 mJ/cm 2 doses of ultraviolet. After 24 hours of HaCat culture, cell viability was detected by MTT assay, and cell cycle was observed by flow cytometry. The protein levels of Caspase-3 and Caspase-9 were detected by western blotting. Results The results of MTT showed that the survival rate of HaCat cells was increased with the increase of Salidroside concentration. Flow cytometry suggested the S peak of HaCat cells increased with the increasing concentration of Salidroside. The S peak of group VI was higher than that of Group II. Western blotting revealed that the protein expression of Caspase-3/9 decreased in the Salidroside group with high concentration. Conclusion Salidroside can reduce HaCat cell apoptosis induced by UVB through inhibiting the expression of Caspase-3 and Caspase-9 protein. |
| Key words: Salidroside ultraviolet B cell proliferation Caspase-3 Caspase-9 |
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