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刘蓉芳,毛以林,谭雄,张辉,毛湘屏,杨柳,陈志成.心康冲剂改善慢性心衰模型大鼠心肌凋亡及调控Caspase-3/9的表达[J].湖南中医药大学学报英文版,2019,39(8):948-951.[Click to copy
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| 心康冲剂改善慢性心衰模型大鼠心肌凋亡及调控Caspase-3/9的表达 |
| 刘蓉芳,毛以林,谭雄,张辉,毛湘屏,杨柳,陈志成 |
| (江门市五邑中医院, 广东 江门 529000;湖南中医药大学第二附属医院, 湖南 长沙 410005) |
| 摘要: |
| 目的 探讨心康冲剂对心衰大鼠Caspase-3、Caspase-9表达的调控作用。方法 58只SD大鼠分为正常组10只，模型组、心康组及对照组各16只，造模完后，心康组给予心康冲剂溶液0.5 g/kg灌服，对照组予芪苈强心胶囊溶液0.06 g/kg灌服。采用心脏彩超检测心功能；心脏切片行TUNNEL染色法观察心肌细胞凋亡；心肌组织采用Real-time PCR法及免疫组化法检测Caspase-3、Caspase-9基因及蛋白质表达。结果 与正常组比，模型组大鼠Caspase-3及Caspase-9的mRNA与蛋白表达显著增加（P<0.01），TUNNEL染色观察细胞凋亡明显增多；与模型组比较，心康组大鼠心肌凋亡减轻， Caspase-9 mRNA、Caspase-3 mRNA及蛋白明显下降（P<0.01）；与对照组相比，心康组心肌细胞凋亡相对降低，Caspase-3蛋白表达下降（P<0.05）。结论 心康冲剂可调控下调Caspase-3、Caspase-9表达抗心衰。 |
| 关键词: 心肌凋亡 Caspase-3 Caspase-9 慢性心衰 心康冲剂 |
| DOI：10.3969/j.issn.1674-070X.2019.08.004 |
| Received:September 10, 2017 |
| 基金项目:湖南省自然科学基金（2016JJ4068）；湖南省中医药科研计划（201610）；国家重点实验室中医诊断学开放基金（2015ZYZD11）；2016年研究生创新科研课题（2016CX08）。 |
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| Xinkang Granule Improves Myocardial Apoptosis and the Expression of Caspase-3/9 in Rats with Chronic Heart Failure |
| LIU Rongfang,MAO Yilin,TAN Xiong,ZHANG Hui,MAO Xiangping,YANG Liu,CHEN Zhicheng |
| (Wuyi Hospital of Traditional Chinese Medicine, Jiangmen, Guangdong 529000, China;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China) |
| Abstract: |
| Objective To investigate the regulatory effects of Xinkang Granule (XKG) on the rats with chronic heart failure (CHF) for the expression of Caspase-3 and Caspase-9. Methods A total of 58 SD rats were randomly divided into a normal group (n=10), a model group, a XKG group, and a control group, with 16 rats in each group. After the success of the modeling, the XKG group was given XKG solution at 0.5 g/kg, and the control group was given Qili Qiangxin Capsule (QLQXC) solution at 0.06 g/kg. Cardiac function was measured by echocardiography; Heart slices were stained by TUNNEL to observe myocardial apoptosis. The expression of gene and protein of Caspase-3, Caspase-9 were detected by Real-time PCR and immunohistochemistry. Results Compared with the normal group, the gene and protein of Caspase-3, Caspase-9 in the model group significantly increased (P<0.01). TUNNEL observed significant increase of apoptosis. Compared with the model group, the rats in XKG group showed alleviated myocardial apoptosis, and the Caspase-3, Caspase-9 mRNA and protein expression were significantly reduced (P<0.01). Compared with the control group, the myocardial apoptosis in the XKG group was relatively decreased, and Caspase-3 decreased (P<0.05). Conclusion XKG has the function of anti-heart failure by regulating and controlling the expression of Caspase-3 and Caspase-9. |
| Key words: myocardial fibrosis Caspase-3 Caspase-9 chronic heart failure Xinkang Granule |
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