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金剑,劳嘉,钟灿,贺炜,蔡媛,周伟良,邓敏,张水寒.基于仿生提取法研究黄精蒸制过程抗氧化活力动态变化[J].湖南中医药大学学报英文版,2019,39(7):837-840.[Click to copy
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| 基于仿生提取法研究黄精蒸制过程抗氧化活力动态变化 |
| 金剑,劳嘉,钟灿,贺炜,蔡媛,周伟良,邓敏,张水寒 |
| (湖南省中医药研究院中药研究所, 湖南 长沙 410013;绿之韵生物工程集团有限公司, 湖南 长沙 410329;湖南神舟中药饮片有限公司, 湖南 慈利 427200;长沙医学院, 湖南 长沙 410219;湖南省中医药研究院中药研究所, 湖南 长沙 410013;湖南省2011数字中医药协同创新中心, 湖南 长沙 410208) |
| 摘要: |
| 目的 研究黄精蒸制加工过程的抗氧化活力动态变化，探讨黄精九蒸九制的机理。方法 对比仿生提取和化学提取黄精样品的抗氧化活力，采用ABTS、FRAP和AAPH法测定不同蒸制次数黄精样品抗氧化活力动态变化。结果 仿生提取的抗氧化活力整体更强，最高是化学提取的2.97倍。抗氧化活力随着蒸制次数增加而增强。ABTS、FRAP和AAPH 3种方法的测定结果显示，黄精蒸制后抗氧化活力较鲜黄精分别增强了1.35、2.54和2.53倍。结论 仿生提取更能全面的代表黄精的抗氧化能力，抗氧化活力的增强是黄精炮制增效的一个很好解释。 |
| 关键词: 黄精 仿生提取 蒸制 抗氧化 |
| DOI：10.3969/j.issn.1674-070X.2019.07.010 |
| Received:January 09, 2019 |
| 基金项目:中央引导地方科技发展专项资金（2018XF5048）；多花黄精内生菌结构多样性及其与药效成分的相关性研究（201705）；湖南省中药材全产业链发展专项（S2017SFXYZY0019）。 |
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| Dynamic Changes of Antioxidant Activity of Polygonati Rhizoma During Steaming Process Based on Bionic Extraction |
| JIN Jian,LAO Jia,ZHONG Can,HE Wei,CAI Yuan,ZHOU Weiliang,DENG Min,ZHANG Shuihan |
| (Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha, Hunan 410013, China;Resgreen Biological Engineering Group Co. Ltd., Changsha, Hunan 410329, China;Hunan Shenzhou Chinese Medicine Decoction Pieces Co. Ltd., Cili, Hunan 427200, China;Changsha Medical University, Changsha, Hunan 410219, China;Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha, Hunan 410013, China;Hunan 2011 Collaboration and Innovation Center for Digital Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the dynamic changes of antioxidant activity of Polygonati Rhizoma during the process of steaming and to explore the mechanism of nine-time steaming and nine-time processing of Polygonati Rhizoma. Methods Polygonati Rhizoma samples with different steaming times were extracted by biomimetic and chemical extraction. The antioxidant activity was determined by ABTS, FRAP and AAPH methods. Results The antioxidant activity of the bionic extraction was stronger overall, and the highest was 2.97 times to that of the chemical extraction. The antioxidant activity was increased as the times of steaming increased. According to the results of ABTS, FRAP and AAPH, the antioxidant activity of steamed Polygonati Rhizoma was respectively 1.35, 2.54 and 2.53 times higher than that of the fresh samples. Conclusion Bionic extraction can more comprehensively represent the antioxidant capacity of Polygonati Rhizoma, and the enhancement of the antioxidant activity is a good explanation for the enhanced efficacy of Polygonati Rhizoma after the steaming process. |
| Key words: Polygonati Rhizoma bionic extraction steaming antioxidant |
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