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黄淑梅,张䶮之,阿丽米热·阿克木江,木拉提·克扎衣别克.阿尔泰瑞香提取物的体外抗炎及抗氧化活性[J].湖南中医药大学学报英文版,2019,39(7):832-836.[Click to copy
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| 阿尔泰瑞香提取物的体外抗炎及抗氧化活性 |
| 黄淑梅,张䶮之,阿丽米热·阿克木江,木拉提·克扎衣别克 |
| (新疆医科大学药学院, 新疆 乌鲁木齐 830001;伊犁哈萨克自治州中医医院, 新疆 伊宁 835000;伊犁州友谊医院药剂科, 新疆 伊宁 835000) |
| 摘要: |
| 目的 探讨阿尔泰瑞香二氯甲烷提取物（Da-Dm）的体外抗炎及抗氧化活性。方法 以不同浓度（1、6、30 μg/mL）的Da-Dm作用于脂多糖（LPS，1 μg/mL）诱导的RAW264.7小鼠巨噬细胞，采用CCK-8法测定Da-Dm对细胞增殖的影响；Griess法检测细胞上清液中一氧化氮（NO）的释放量；ELISA法检测细胞上清液中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）的分泌量。以维生素C为对照，采用FRAP法、DPPH法评价Da-Dm总抗氧化能力。结果 Da-Dm在浓度为30 μg/mL及以下时对RAW264.7细胞增殖没有影响。与LPS组比较，1~30 μg/mL的Da-Dm可明显降低LPS诱导的RAW264.7细胞分泌炎症因子NO、IL-1β、IL-6和TNF-α的含量（P<0.05，P<0.01），并呈现浓度依赖性。Da-Dm对DPPH自由基有较好的清除能力，其清除率的IC50为318.1 μg/mL，在FRAP实验中其对Fe2+离子具有较强的还原能力。结论 Da-Dm可以抑制LPS诱导的RAW264.7细胞炎症反应，它的抗炎作用可能与抑制NO、IL-1β、IL-6和TNF-α的释放有关。Da-Dm具有较好的抗氧化活性。 |
| 关键词: 阿尔泰瑞香 二氯甲烷提取物 RAW264.7细胞 炎症介质 抗氧化作用 |
| DOI：10.3969/j.issn.1674-070X.2019.07.009 |
| Received:March 23, 2019 |
| 基金项目:国家自然科学基金（81360499）；新疆维吾尔自治区青年科技创新人才培养工程（QN2016YX0759）。 |
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| Antioxidant and Anti-inflammatory Activities in Vitro of the Extracts from Daphne altaica Pall |
| HUANG Shumei,ZHANG Yanzhi,AKEMUJIANG Alimire,KEZHAYIBIEKE Mulati |
| (College of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang 830001, China;Department of Pharmacy, The Friendship Hospital of Ili Kazak Autonomous Prefecture, Yining, Xinjiang 835000, China;Traditional Chinese Medicine Hospital of Ili Kazak Autonomous Prefecture, Yining, Xinjiang 835000, China) |
| Abstract: |
| Objective To explore the in vitro anti-inflammatory and antioxidant activities of the dichloromethane extracts from Daphne altaica Pall (Da-Dm). Methods Mice RAW264.7 cells induced by lipopolysaccharides (LPS, 1 μg/mL) were treated with different concentrations of Da-Dm (1, 6, 30 μg/mL) respectively. The proliferation of RAW264.7 cells was determined by CCK-8 method; the output of nitric oxide (NO) in the cell supernatant was evaluated by Griess reagent assay; the secretion volumes of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6) were detected by ELISA. The total antioxidant activity of the Da-Dm was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and ferric reducing-antioxidant power (FRAP) assay, and vitamin C (Vc) was used as a positive control. Results Da-Dm with the concentration of 30 μg/mL and below had no influence on the the proliferation of RAW264.7 cells. Compared with the LPS group, 1-30 μg/mL of Da-Dm in the LPS-induced RAW264.7 cells greatly inhibited their release of inflammatory mediators, such as NO, IL-1β, IL-6 and TNF-α (P<0.05, P<0.01), in a dose-dependent manner. The Da-Dm exhibited good DPPH radical cleaning power and good Fe2+ reducing power. The median inhibitory concentration (IC50) of scavenging DPPH radical was 318.1 μg/mL. Conclusion Da-Dm can inhibit LPS-induced inflammatory response in RAW264.7 cells, and its anti-inflammatory effect may be related to the reduction of the release of inflammatory cytokines, like NO, IL-1β, IL-6 and TNF-α. The antioxidant activity of Da-Dm is strong. |
| Key words: Daphne altaica Pall dichloromethane extracts RAW264.7 cells inflammatory cytokines antioxidant |
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