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鲜瑶瑶,周俭,曾光,王莘智,刘沙,徐豫湘.熊果酸对胶原诱导性关节炎大鼠关节炎症相关因子mRNA表达的影响[J].湖南中医药大学学报英文版,2019,39(6):689-692.[Click to copy
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| 熊果酸对胶原诱导性关节炎大鼠关节炎症相关因子mRNA表达的影响 |
| 鲜瑶瑶,周俭,曾光,王莘智,刘沙,徐豫湘 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 研究熊果酸（ursolic acid，UA）对胶原诱导性关节炎（collageninduced arthritis，CIA）大鼠关节X线及关节炎症相关因子mRNA表达的影响。方法 SD大鼠采取皮下注射牛Ⅱ型胶原加弗氏不完全佐剂法建立CIA模型，取造模成功后大鼠随机分为CIA模型组、UA低剂量组、UA高剂量组、甲氨喋呤（methotrexate，MTX）组、UA+MTX组并予以对应干预。另取6只正常大鼠为对照的空白组。初次免疫30 d断颈处死大鼠，关节行X线摄片，再取下大鼠炎症关节组织匀浆，提取RNA，荧光定量PCR法检测IL-17A、IL-10、IL-6、TNF-α、IL-1β、TGF-β的mRNA表达。结果 与CIA模型组相比，UA高剂量组、MTX组、UA+MTX组X线评分减低（P<0.05或P<0.01）；各治疗组IL-17A、IL-6、TNF-α、IL-1β的mRNA表达降低（P<0.01或P<0.05）；各治疗组IL-10、TGF-β因子的mRNA表达增高（P<0.01或P<0.05）。结论 UA可明显缓解CIA大鼠关节炎症，改善关节X线评分，其机制可能与调节炎症因子的产生，维持组织促炎因子与抑炎因子的平衡有关。 |
| 关键词: 熊果酸 类风湿关节炎 胶原诱导性关节炎 X线评分 炎症因子 |
| DOI：10.3969/j.issn.1674-070X.2019.06.003 |
| Received:September 19, 2018 |
| 基金项目:国家自然科学基金青年科学基金项目（81403206）；湖南省教育厅重点项目（18A209）；湖南省中医药管理局重点项目（201919）；中国博士后基金委面上项目（2014M560646）；湖南省研究生科研创新项目（CX2017B447）。 |
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| Effects of Ursolic Acid on mRNA Expression of Joint Inflammation-related Factors in Collagen-induced Arthritis Rats |
| XIAN Yaoyao,ZHOU Jian,ZENG Guang,WANG Shenzhi,LIU Sha,XU Yuxiang |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To investigate the effects of ursolic acid (UA) on the articular X-ray and mRNA expressions of joint inflammation-related factors in collagen-induced arthritis (CIA) rats. Methods SD rats were given subcutaneous injection of bovine type Ⅱ collagen plus Freund's incomplete adjuvant for modeling. After successful modeling, the rats were randomly divided into a CIA model group, a UA low-dose group, a UA high-dose group, a methotrexate (MTX) group and a UA + MTX group and they were given corresponding intervention. Another 6 normal rats were selected as the control group. Rats were sacrificed by neck breaking after 30 d of primary immunization, and the joints were taken X-ray. The tissue homogenate of rat's inflammatory joint was removed and RNA was extracted. The expressions of IL-17A, IL-10, IL-6, TNF-α, IL-1β, TGF-β and mRNA were detected by fluorescence quantitative PCR. Results Compared with the CIA model group, the X-ray scores of the UA high-dose group, the MTX group, and the UA + MTX group were decreased (P<0.05 or P<0.01); the mRNA expressions of IL-17A, IL-6, TNF-α and IL-1β in each treatment group were decreased (P<0.01 or P<0.05); the mRNA expressions of IL-10 and TGF-β in each treatment group were increased (P<0.01 or P<0.05). Conclusion UA can significantly alleviate joint inflammation in CIA rats and improve the articular X-ray score. The mechanism may be related to regulating the production of inflammatory factors and maintaining the balance between tissue pro-inflammatory factors and anti-inflammatory factors. |
| Key words: ursolic acid rheumatoid arthritis collagen-induced arthritis X-ray score inflammatory factor |
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