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白玉琢,徐菁,陈洁,张佳怡,许玥,鲁曼,李欣怡,霍泽军,张莉.电针“委中”对多裂肌损伤模型大鼠钙调蛋白信号通路的影响[J].湖南中医药大学学报英文版,2019,39(4):503-506.[Click to copy
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| 电针“委中”对多裂肌损伤模型大鼠钙调蛋白信号通路的影响 |
| 白玉琢,徐菁,陈洁,张佳怡,许玥,鲁曼,李欣怡,霍泽军,张莉 |
| (北京中医药大学针灸推拿学院, 北京 10029;北京大学第三医院中医科, 北京 100191) |
| 摘要: |
| 目的 探讨多裂肌损伤后，电针"委中"干预对多裂肌损伤过程中钙调蛋白信号通路的影响。方法 SD大鼠随机分为空白组、模型1 d组、模型3 d组、电针1 d组、电针3 d组，每组8只。电针组双侧"委中"电针，于治疗的第1、3天各组同步取材，采用酶联免疫吸附法检测多裂肌、脊髓、海马组织中CaM、CaMKⅡ、iNOS的含量。结果 模型1 d和3 d组，多裂肌、脊髓、海马CaM、CaMKⅡ含量显著高于空白组（P<0.01）；多裂肌、脊髓、海马iNOS含量高于空白组（P<0.05或P<0.01）。电针1 d后，多裂肌、脊髓、海马CaM、CaMKⅡ、iNOS含量显著低于模型1 d组（P<0.05或P<0.01）。电针3 d后，多裂肌、脊髓、海马CaM、CaMKⅡ、iNOS含量低于模型3 d组（P<0.05或P<0.01）。电针3 d后，脊髓、海马CaM、CaMKⅡ及多裂肌CaMKⅡ含量显著低于电针1 d组（P<0.01），多裂肌CaM、iNOS和脊髓、海马iNOS含量与电针1 d组比较差异无统计学意义（P>0.05）。结论 多裂肌损伤早期，电针"委中"干预可通过抑制CaM、CaMKⅡ的过度激活，减少组织中iNOS的过多产生，减轻组织炎症损伤。 |
| 关键词: 多裂肌 脊髓 电针 委中 海马 钙调蛋白 |
| DOI：10.3969/j.issn.1674-070X.2019.04.014 |
| Received:December 14, 2018 |
| 基金项目:国家自然科学基金项目（81574052） |
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| Effects of Electroacupuncture Weizhong (BL40) on Calmodulin Signaling Pathway in Rat Model of Multifidus Injury |
| BAI Yuzhuo,XU Jing,CHEN Jie,ZHANG Jiayi,XU Yue,LU Man,LI Xinyi,HUO Zejun,ZHANG Li |
| (School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China;Department of Traditional Chinese Medicine, Peking University Third Hospital, Beijing 100191, China) |
| Abstract: |
| Objective To investigate the effects of electroacupuncture (EA) Weizhong (BL40) intervention on calmodulin signaling pathway in the process of multifidus muscle injury. Methods SD rats were randomly divided into the blank group, model 1d group, model 3d group, EA 1d group and EA 3d group, with 8 rats in each group. For the EA group, EA was performed at bilateral Weizhong (BL40). Materials were collected on the first and third day of treatment among each group. The contents of CaM, CaMKⅡ and iNOS in multifidus muscles, spinal cord and hippocampus were detected by ELISA. Results The contents of CaM and CaMK Ⅱ in the hippocampus, spinal cord, hippocampus and multifidus muscles in the model 1d and 3d group were significantly higher than those in the blank group (P<0.01), while the iNOS contents in the multifidus muscles, spinal cord and hippocampus were significantly higher than those in the blank group (P<0.05 or P<0.01). After 1 day of EA, the contents of CaM, CaMKⅡ and iNOS in the multifidus muscles, spinal cord and hippocampus were significantly lower than those in the model 1d group (P<0.05 or P<0.01). After 3 days of EA, the contents of CaM, CaMKⅡ and iNOS in the multifidus muscles, spinal cord and hippocampus were significantly lower than those in the model 3d group (P<0.05 or P<0.01). After 3 days of EA, the contents of CaM, CaMKⅡ in the spinal cord and hippocampus and the CaMKⅡ in the multifidus muscles were significantly lower than those in the EA 1d group (P<0.01). The contents of CaM and iNOS in multifidus muscles, and iNOS in the spinal cord and hippocampus were not significantly different when compared with EA 1d group (P>0.05). Conclusion In the early stage of multifidus muscles injury, EA Weizhong (BL40) intervention can reduce the excessive activation of CaM and CaMK Ⅱ, reduce the excessive production of iNOS in tissues, and alleviate tissue inflammation injury. |
| Key words: multifidus muscle spinal cord electroacupuncture Weizhong (BL40) hippocampus almodulin |
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