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曹玉成,王哲,宋炜熙.补肾填髓方对阿尔茨海默病模型大鼠学习记忆能力及线粒体氧化应激的影响[J].湖南中医药大学学报英文版,2019,39(4):459-464.[Click to copy
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| 补肾填髓方对阿尔茨海默病模型大鼠学习记忆能力及线粒体氧化应激的影响 |
| 曹玉成,王哲,宋炜熙 |
| (祁阳县中医医院, 湖南 永州 426100;中南大学湘雅二医院, 湖南 长沙 410013;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察补肾填髓方对阿尔茨海默病（Alzheimer's disease，AD）模型大鼠学习记忆能力及线粒体氧化应激的影响。方法 50只大鼠先用Y迷宫初筛，再按旷场实验得分随机分成正常组、模型组、假手术组、中药组、西药组。假手术组两侧侧脑室注射β淀粉样蛋白（Aβ）42~1，余下除正常组外均以双侧侧脑室注射（Aβ）1-42复制AD模型。造模后中药组灌服补肾填髓方，西药组灌服多奈哌齐，其他组灌服等容量生理盐水，连续干预28 d。Morris水迷宫检测大鼠学习记忆能力，酶联免疫吸附方法（ELISA）检测大鼠脑皮质及血清超氧化物歧化酶（superoxide dismufase，SOD）活性，硫代巴比妥酸法（TBA）检测大鼠脑皮质及血清丙二醛（malondialdehyde，MDA）活性，透射电镜观测海马线粒体超微结构。结果 与模型组比较，中药组和西药组大鼠水迷宫逃避潜伏期缩短，在原平台象限游泳时间及距离的百分比增加，差异均有显著统计学意义（P<0.05或P<0.01）。与模型组比较，中药组SOD活性升高，MAD活性减低（P<0.05，P<0.01）；西药组的SOD活性升高，MDA活性降低（P<0.01）；中药组及西药组的海马线粒体超微结构病理损害改善。结论 补肾填髓方可以改善AD大鼠的学习记忆能力，其机制可能与改善线粒体氧化应激有关。 |
| 关键词: 阿尔茨海默病 补肾填髓方 氧化应激 线粒体 |
| DOI：10.3969/j.issn.1674-070X.2019.04.006 |
| Received:September 05, 2018 |
| 基金项目:国家自然科学基金项目（81373705）；湖南省自然科学基金资助项目（13JJ3030） |
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| Effects of Bushen Tiansui Fang on Learning and Memory Abilities and Oxidative Stress of Mitochondria of Alzheimer's Disease Model Rat |
| CAO Yucheng,WANG Zhe,SONG Weixi |
| (Qiyang Hospital of Traditional Chinese Medicine, Yongzhou, Hunan 426100, China;The Second Xiangya Hospital of Central South University, Changsha, Hunan 410013, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of Bushen Tiansui Fang on learning and memory abilities and oxidative stress of mitochondria of Alzheimer's disease (AD) model rats. Methods A total of 50 rats were initially screened by Y maze, and then divided into the normal group, the model group, the sham operation group, the Chinese medicine group, the Western medicine group randomly according to the score of open filed test. Aβ1-42 (amyloid β-protein) were injected into bilateral ventricle of rats to duplicate AD model, and Aβ42-1 were injected into the sham operation group rats as the same dose. After modeling, the Chinese medicine group and the Western medicine group were administered by gavage once a day for 4 weeks with Bushen Tiansui Fang and Donepezil respectively. At the same time, the remaining groups were given by gavage with the same capacity amount of normal saline, for continuous 28 days' intervention. The abilities of learning and memory were assessed with Morris water maze. The activity of superoxide dismutase (SOD) in prefrontal cortex and serum of rats was detected by enzyme linked immunosorbent assay (ELISA). The activity of malondialdehyde (MDA) in prefrontal cortex and serum of rats was detected by thiobarbituric acid (TBA) method. The ultrastructures of hippocampal mitochondria were observed with transmission electron microscopy. Results Compared with the model group, the escape latent period shortened in the Chinese medicine group and the Western medicine group, while the percentage of swimming time and swimming distance in the original platform quadrant increased (P<0.01). Compared with the model group, the SOD activity increased and MAD activity decreased in Chinese medicine group (P<0.05; P<0.01); the SOD activity increased and MDA activity decreased in the Western medicine group (P<0.01); the mitochondrial ultrastructure pathological changes in the Chinese medicine group and the Western medicine group were ameliorated. Conclusion The learning and memory abilities of AD rats can be improved by Bushen Tiansui Fang, and its mechanism may be related to the improvement of mitochondrial oxidative stress. |
| Key words: Alzheimer's disease Bushen Tiansui Fang oxidative stress mitochondria |
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