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张又玮,覃艮艳,彭晓芳,彭俊,潘坤,周亚沙,彭清华.密蒙花滴眼液对去势雄兔干眼泪腺组织中ICAM-1、IL-6、IL-17表达的影响[J].湖南中医药大学学报英文版,2019,39(4):448-452.[Click to copy
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| 密蒙花滴眼液对去势雄兔干眼泪腺组织中ICAM-1、IL-6、IL-17表达的影响 |
| 张又玮,覃艮艳,彭晓芳,彭俊,潘坤,周亚沙,彭清华 |
| (石家庄市中医院, 河北 石家庄 050000;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察不同浓度密蒙花滴眼液对雄兔干眼泪腺组织中炎性细胞因子：细胞间黏附因子-1（intercellular cell adhesion molecule-1，ICAM-1）、白细胞介素-6（interleukin-6，IL-6）、白细胞介素-17（interleukin-17，IL-17）表达的影响，从而探讨其疗效。方法 将36只成年新西兰雄兔随机分为空白组（A），模型组（B），密蒙花滴眼液低（C）、中（D）、高（E）浓度组，睾酮组（F）组，每组6只。除A组外，其余各组均切除双侧睾丸及附睾，造模成功后，C、D、E组开始双眼滴用滴眼液，F组注射丙酸睾酮注射液。术前及治疗后第4周各组雄兔均测定1次泪膜破裂时间（tear break-up time，BUT）和泪液分泌功能（Schirmer Ⅰ test，SIT）。治疗后第4周处死实验用兔，并摘取其双侧泪腺，采用免疫组化的方法检测ICAM-1、IL-6与IL-17的表达。结果 （1） SIT和BUT：B组自身前后对比，治疗后第4周：B组与C、D、E、F组相比，E组与C、D组相比，F组与C、D、E组相比，差异均有统计学意义（P<0.05或P<0.01）；（2） ICAM-1、IL-6与IL-17电镜下表达的结果：A、F组未见明显表达；B组可以见到大量棕黄色颗粒存在细胞浆与细胞膜中；C、D、E组可见散在的表达；（3）治疗后第4周，组间ICAM-1、IL-6与IL-17的表达相比较：B组与其余各组相比，E组与C、D组相比，差异均有统计学意义（P<0.05或P<0.01）。结论 密蒙花滴眼液具有拟雄激素的作用，可以抑制炎性细胞因子ICAM-1、IL-6与IL-17的表达，疗效弱于雄激素。不同浓度的密蒙花滴眼液中，高浓度的疗效最佳。 |
| 关键词: 密蒙花滴眼液 干眼 泪腺 炎性细胞因子 |
| DOI：10.3969/j.issn.1674-070X.2019.04.004 |
| Received:October 30, 2018 |
| 基金项目:国家自然科学基金面上资助项目（30772824，81574031）；湖南省高层次卫生人才"225"工程培养项目资助；湖南省科技厅科研基金资助项目（2015SF2016-6）；湖南省发展改革委科研基金资助项目（湘发改投资[2014]658号）；长沙市科技计划重大专项（K1501014-31）；湖南中医药大学研究生创新项目（2016CX19）；国家中医药管理局中医眼科学重点学科建设项目；中医药防治眼耳鼻喉疾病湖南省重点实验室建设项目；湖南中医药大学中医学国内一流建设学科 |
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| Effects of Butterflybush Flower Eye Drops on Expression of ICAM-1,IL-6 and IL-17 in the Lacrimal Gland Cells of Castrated Male Rabbits with Dry Eye Disease |
| ZHANG Youwei,QIN Genyan,PENG Xiaofang,PENG Jun,PAN Kun,ZHOU Yasha,PENG Qinghua |
| (Shijiazhuang Traditional Chinese Medicine Hospital, Shijiazhuang, Hebei 050000, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of different concentration of butterflybush flower eye drops on the expression of inflammatory factors:intercellular cell adhesion molecule-1 (ICAM-1), interleukin-6 (IL-6) and interleukin-17 (IL-17) in the acrimal gland cells of castrated male rabbits with dry eye disease, then to discuss the curative effects. Methods A total of 36 male adult New Zealand rabbits into 6 groups, with 6 rabbits in each group:the blank group (A), the model group (B), the low concentration group (C), the middle concentration group (D), the high concentration group (E), the testosterone group (F). Except group A, all the other male rabbits were excised bilateral testicles and epididymis. After the modeling, group C, group D and group E began to use the relative concentration of butterflybush flower eye drops in the eyes. Group F was injected Testosterone Propionate Injection. All the male rabbits need to be determined break-up time (BUT) and tear secretion function (Schirmer I, SIT) before the operation and the 4th week after the treatment. All the experimental rabbits were killed and the lacrimal gland of the both eyes were extracted the 4th week after the treatment. Immunohistochemical method was used to detect the expression of ICAM-1, IL-6 and IL-17. Results (1) SIT and BUT:for self-comparison in the group B, the 28th day after treatment, comparison between the group B, and group C, D, E, F, comparison between group E, and group C, D, comparison between group F, and group C, D F, the differences were statistically significant (P<0.05); (2) The results of the expression of ICAM-1, IL-6 and IL-17 under electron microscope:There was no obvious expression in group A and group F; A large number of brown and yellow granules were found in the cytoplasm and cell membrane in group B; There was sparse expression in group C, group D and group E; (3) The comparison of ICAM-1, IL-6 and IL-17 between groups in the 4th week after the treatment:for comparison between group B and other groups, and comparison between group E and group C, D, the differences were statistically significant (P<0.05). Conclusion Butterflybush flower eye drops have the similar effects of androgen, and can inhibit the expression of inflammatory cytokines ICAM-1, IL-6 and IL-17, and the effect is weaker than that of androgen. Among the different concentrations of eye drops, the high concentration is the best. |
| Key words: butterflybush flower eye drops dry eye disease lacrimal gland inflammatory factors |
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