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杨筱倩,陈仙蕾,杨仁义,唐标,刘晓丹,邓常清,黄小平.冰片配伍黄芪甲苷与三七总皂苷抗脑缺血再灌注损伤有效剂量的研究[J].湖南中医药大学学报英文版,2019,39(4):441-447.[Click to copy
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| 冰片配伍黄芪甲苷与三七总皂苷抗脑缺血再灌注损伤有效剂量的研究 |
| 杨筱倩,陈仙蕾,杨仁义,唐标,刘晓丹,邓常清,黄小平 |
| (湖南中医药大学分子病理实验室, 中西医结合心脑疾病防治湖南省重点实验室, 细胞生物学与分子技术湖南省高校重点实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨冰片配伍黄芪甲苷（astragalosides Ⅳ，AST Ⅳ）和三七总皂苷（panax notoginseng saponins，PNS）抗脑缺血再灌注损伤的有效剂量。方法 采用U6*（64）均匀设计实验，设立6个不同剂量的冰片、AST Ⅳ和PNS配伍，予大脑中动脉阻塞（middle cerebral artery occlusion，MCAO）再灌注大鼠灌胃干预，以神经功能评分、脑梗死率、脑含水量、脑组织病理形态、血脑屏障（blood-brain barrier，BBB）通透性评价药物的作用，对效应指标进行多重线性回归分析，确立3种组（成）分的有效配伍剂量，并在此基础上进行验证。结果 均匀设计实验表明，大鼠脑缺血再灌注后，出现明显的神经功能障碍，脑梗死率、脑含水量、神经细胞损伤率和BBB通透性均显著增加。冰片、AST Ⅳ、PNS不同剂量配伍对上述指标均有一定的改善作用；多重线性回归分析表明，冰片7.5 mg/kg、AST Ⅳ10 mg/kg、PNS25 mg/kg配伍为其抗脑缺血再灌注损伤的有效配伍剂量。验证实验显示，3种药物高、低剂量配伍能显著降低脑缺血再灌注大鼠神经功能评分和脑梗死率，3种药物配伍的效应显著优于各药物单用或AST Ⅳ+PNS配伍（P<0.05或P<0.01）。结论 冰片、AST Ⅳ、PNS配伍可增强抗脑缺血再灌注损伤的效应，3种药物的有效配伍剂量为冰片7.5 mg/kg+AST Ⅳ 10 mg/kg+PNS 25 mg/kg。 |
| 关键词: 冰片 黄芪甲苷 三七总皂苷 脑缺血再灌注 配伍剂量 |
| DOI：10.3969/j.issn.1674-070X.2019.04.003 |
| Received:October 03, 2018 |
| 基金项目:国家自然科学基金项目（81573875）；湖南省中医药管理局重点项目（201509）；2016年湖南省研究生科研创新项目（CX2016B372）；2017年度湖南省大学生研究性学习和创新性实验计划项目（1029）；湖南省科技厅科技创新平台与人才计划支持项目（2017SK4005） |
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| Study on the Effective Dosage of Borneol Combined with Astragalosides Ⅳ and Panax Notoginseng Saponins Antagonizing Cerebral Ischemia-Reperfusion Injury |
| YANG Xiaoqian,CHEN Xianlei,YANG Renyi,TANG Biao,LIU Xiaodan,DENG Changqing,HUANG Xiaoping |
| (Molecular Pathology Laboratory, Key Laboratory of Hunan Province for Prevention and Treatment of Integrated Traditional Chinese and Western Medicine on Cardio-Cerebral Diseases, Key Laboratory of Hunan Universities for Cell Biology and Molecular Techniques, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the effective dosage of Borneol combined with Astragalosides Ⅳ (AST Ⅳ) and panax notoginseng saponins (PNS) antagonizing cerebral ischemia-reperfusion injury. Methods According to U6*(64) uniform design experiment, 6 different dosage combinations of Borneol, AST Ⅳ and PNS were set. The middle cerebral artery occlusion (MCAO)-reperfusion rats was administrated. The neurological function score, cerebral infarct volume, brain water content, brain histopathological morphology, blood-brain barrier (BBB) permeability were used to assess the effects of drugs. The effect index was analyzed using multiple linear regression method, thus to ascertain the effective combination dosage of 3 ingredients (components), and the effective combination dosage were exerted to the experimental verification. Results The uniform design experiment results showed that the rats appeared obvious neurological dysfunction, cerebral infarction rate, brain water content, nerve cell injury rate and BBB permeability were all increased after cerebral ischemia-reperfusion. The different dosage combinations of Borneol, AST Ⅳ and PNS could improve the index above. Multiple linear regression analysis showed that the effective combination dosage of Borneol, AST Ⅳ and PNS antagonizing cerebral ischemia-reperfusion injury was Borneol 7.5 mg/kg, AST Ⅳ 10 mg/kg, PNS 25 mg/kg, respectively. Verification experiment results showed that 3 drug combinations with high or low dosage could significantly decrease neurological function score and cerebral infarct volume of cerebral ischemia-reperfusion rats, and the effects of the combinations were significantly better than that of single drug or AST Ⅳ+PNS (P<0.05 or P<0.01). Conclusion The combinations of Borneol, AST Ⅳ and PNS can enhance the effects of anti-cerebral ischemia-reperfusion injury, and the effective combination dosage is Borneol 7.5 mg/kg+AST Ⅳ 10 mg/kg+PNS 25 mg/kg. |
| Key words: Borneol Astragalosides Ⅳ panax notoginseng saponins cerebral ischemia-reperfusion combination dosage |
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