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陈日兰,何彦霖,江海燕,刘泓毅,韦秋娜,罗国馨,彭元霞,朱英.基于尿液代谢组学探究刺血结合药线点灸对急性痛风性关节炎大鼠作用机制研究[J].湖南中医药大学学报英文版,2019,39(2):234-240.[Click to copy
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| 基于尿液代谢组学探究刺血结合药线点灸对急性痛风性关节炎大鼠作用机制研究 |
| 陈日兰,何彦霖,江海燕,刘泓毅,韦秋娜,罗国馨,彭元霞,朱英 |
| (广西中医药大学附属瑞康医院, 广西 南宁 530001;广西中医药大学, 广西 南宁 530001) |
| 摘要: |
| 目的 通过观察刺血结合药线点灸对急性痛风性关节炎模型大鼠尿液代谢组学影响，初步探讨刺血结合药线点灸对急性痛风性关节炎作用机制。方法 将39只SD大鼠随机分3组：空白组、模型组、治疗组，每组13只。模型组仅造模，治疗组造模后给予刺血结合药线点灸治疗。记录各组大鼠的步态、关节肿胀度变化；并运用气相色谱仪与飞行时间质谱仪的联用代谢组学技术分析大鼠尿液代谢产物，进行主成分分析和偏最小二乘方判别分析对数据进行统计。结果 治疗后3组大鼠的步态评价和造模后各时间段的关节肿胀度比较，差异均有统计学意义（P<0.05）；以VIP>1为标准寻找治疗组与模型组中的标志性差异性代谢物16个，以P<0.05及质谱信息再鉴别出标志性差异代谢物α-酮戊二酸、γ-氨基丁酸1、肌醇、半乳糖苷、柠檬酸5个；经MetPA数据库进行二次分析，发现急性痛风性关节炎模型大鼠治疗前后其尿液代谢组学变化涉及代谢通路13条，其中丁酸代谢、三羧酸循环、半乳糖代谢以及丙氨酸、天冬氨酸和谷氨酸代谢这4条代谢途径影响值的临界点>0.10。结论 刺血结合药线点灸可以改善急性痛风性关节炎大鼠步态及关节肿胀度。其作用机制可能是通过影响体内丁酸代谢、三羧酸循环、半乳糖代谢以及丙氨酸、天冬氨酸和谷氨酸代谢通路从而抑制炎症因子转录生成，加速局部代谢循环，起到消炎、镇痛作用。 |
| 关键词: 痛风性关节炎 刺血 药线点灸 代谢组学 |
| DOI：10.3969/j.issn.1674-070X.2019.02.020 |
| Received:May 21, 2018 |
| 基金项目:国家自然科学基金（8146076） |
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| Mechanism of Action of Blood-Pricking Therapy Combined with Medicated Thread Moxibustion in Rats with Acute Gouty Arthritis: A Study Based on Urine Metabolomics |
| CHEN Rilan,HE Yanlin,JIANG Haiyan,LIU Hongyi,WEI Qiuna,LUO Guoxin,PENG Yuanxia,ZHU Ying |
| (Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, China) |
| Abstract: |
| Objective To explore the mechanism of action of blood-pricking therapy combined with medicated thread moxibustion (BPT+MTM) in the treatment of acute gouty arthritis by observing the effect of the above treatment on the urine metabolomics in a rat model of acute gouty arthritis. Methods In this study, a total of 39 Sprague-Dawley rats were randomized into blank group, model group, and treatment group, with 13 rats in each group. Rats in the model group were subjected to modelling only, while the rats in the treatment group were given BPT+MTM after modelling. The changes in gait and joint swelling were recorded for each group; the urine metabolites of the rats were analyzed by gas chromatography coupled to time-of-flight mass spectrometry; meanwhile, principal component analysis and partial least squares-discriminant analysis were carried out to summarize the statistical data. Results There were significant differences between the three groups in gait evaluation results after treatment and the degree of joint swelling at different time points after modelling (P<0.05). Sixteen metabolites with remarkable differences betweer the treatment group and the model group were identified with VIP>1 as the criteria, and five more metabolites (α-ketoglutaric acid, γ-aminobutyric acid 1, inositol, galactoside, and citric acid) with remarkable differences between the above two groups were identified with P<0.05 and mass spectrometric data as the criteria. A secondary analysis of the MetPA database revealed 13 metabolic pathways involved in the changes of urine metabolomics after treatment in the rat model of acute gouty arthritis, among which four pathways (butyric acid metabolism; tricarboxylic acid cycle; galactose metabolism; and alanine, aspartic acid, and glutamic acid metabolism) had a critical point of influence of >0.10. Conclusion BPT+MTM can improve the gait and joint swelling in rats with acute gouty arthritis, the mechanism of which may involve affecting in vivo butyric acid metabolism, tricarboxylic acid cycle, galactose metabolism, and alanine, aspartic acid, and glutamic acid metabolism, thus inhibiting the transcription and production of inflammatory factors, accelerating local metabolism and circulation, and finally reducing the inflammation and pain. |
| Key words: gouty arthritis blood-pricking therapy medicated thread moxibustion metabolomics |
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