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张付民,冉仁国,赵宾宾.酸枣仁汤对慢性睡眠剥夺大鼠视交叉上核生物钟基因Clock和Bmal1表达的影响[J].湖南中医药大学学报英文版,2019,39(1):19-22.[Click to copy
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| 酸枣仁汤对慢性睡眠剥夺大鼠视交叉上核生物钟基因Clock和Bmal1表达的影响 |
| 张付民,冉仁国,赵宾宾 |
| (湖北省恩施州中心医院, 湖北 恩施 445000;湖北中医药大学基础医学院, 湖北 武汉 430065) |
| 摘要: |
| 目的 观察酸枣仁汤对慢性睡眠剥夺大鼠视交叉上核（uprachiasmatic nucleus，SCN）生物钟基因Clock和Bmal1蛋白表达的影响。方法 将实验大鼠随机分为空白组、模型组、舒乐安定组和酸枣仁汤组，除空白组外，其他各组用多平台水环境法制定慢性睡眠剥夺模型并予以相应干预。采用自主活动测试仪记录大鼠自主活动时间，免疫组化和Western blot检测SCN中Clock和Bmal1蛋白表达水平。结果 与空白组比较，模型组大鼠自主活动时间TL（光照时段4个点之和）、TD（黑暗时段4个点之和）、TALL（光照+黑暗8个时段之和）明显增加（P<0.01）；SCN中Clock和Bmal1蛋白表达水平下降（P<0.01）。干预后，与模型组比较，舒乐安定组和酸枣仁汤组大鼠TL、TD、TALL均减少（P<0.01或P<0.05）。舒乐安定组和酸枣仁汤组大鼠SCN中Clock和Bmal1蛋白表达上升（P<0.01或P<0.05）。结论 酸枣仁汤能改善慢性睡眠剥夺大鼠的睡眠状态，其机制可能与酸枣仁汤调节大鼠SCN中Clock和Bmal1的表达有关。 |
| 关键词: 视交叉上核 酸枣仁汤 睡眠剥夺 生物钟基因 Clock Bmal1 自主活动时间 |
| DOI：10.3969/j.issn.1674-070X.2019.01.005 |
| Received:May 31, 2018 |
| 基金项目:国家自然科学基金项目（81703973）；湖北省教育厅科研项目（Q20172003）。 |
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| Effect of Suanzaoren Decoction on Expression of Circadian Genes Clock and Bmal1 in the Suprachiasmatic Nucleus in Chronically Sleep-Deprived Rats |
| ZHANG Fumin,RAN Renguo,ZHAO Binbin |
| (Enshi Central Hospital of Hubei Province, Enshi, Hubei 445000;School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, China) |
| Abstract: |
| Objective To observe the effect of Suanzaoren Decoction on the protein expression of circadian genes Clock and Bmal1 in the suprachiasmatic nucleus (SCN) in chronically sleep-deprived rats.Methods The experimental rats were randomly divided into blank group, model group, Eurodin group, and Suanzaoren Decoction group. All rats except those in the blank group were subjected to a multi-platform water bath to establish a model of chronic sleep deprivation, and each group received the corresponding treatment. An autonomic activity recorder was used to record the time of autonomic activities of the rats. Immunohistochemistry and Western blot were used to measure the protein expression of Clock and Bmal1 in the SCN.Results Compared with the blank group, the model group showed significantly increased TL (sum of automatic activity time recorded at four time points in the light), TD (sum of automatic activity time recorded at four time points in the dark), and TALL(sum of automatic activity time recorded at eight time points in the light and dark) (P<0.01), and significantly decreased protein expression levels of Clock and Bmal1 in the SCN (P<0.01). Compared with the model group, the Eurodin group and Suanzaoren Decoction group showed significantly decreased TL, TD, and TALL after administration (P<0.01 or P<0.05), and significantly increased protein expression levels of Clock and Bmal1 in the SCN (P<0.01 or P<0.05).Conclusion Suanzaoren Decoction can improve the sleep state of chronically sleep-deprived rats, which may be related to its regulation of the expression of Clock and Bmal1 in the SCN of rats. |
| Key words: suprachiasmatic nucleus Suanzaoren Decoction sleep deprivation circadian gene Clock Bmal1 time of autonomic activity |
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