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熊苏慧,夏伯候,雷思敏,李诗卉,谢明霞,李亚梅,林艳.基于低共熔溶剂提取千斤拔多糖[J].湖南中医药大学学报英文版,2018,38(9):1003-1008.[Click to copy
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| 基于低共熔溶剂提取千斤拔多糖 |
| 熊苏慧,夏伯候,雷思敏,李诗卉,谢明霞,李亚梅,林艳 |
| (湖南中医药大学, 湖南 长沙 410208;湘产大宗药材品质评价湖南省重点实验室, 湖南 长沙 410208;中药有毒物质防控技术湖南省工程实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 采用一种新型绿色溶剂低共熔溶剂（deep eutectic solvents，DESs）提取千斤拔多糖，并对其提取工艺及提取效率进行考察。方法 采用响应面法，优化千斤拔多糖提取工艺，并以水提醇沉法评价DESs提取效率。结果 由氯化胆碱和1，3-丁二醇合成的低共熔溶剂最适合于千斤拔多糖的提取。经响应面法优化后，在提取温度70℃、水比例11%、提取时间41 min、液固比11 mL/g下，千斤拔多糖提取率可达2.47%，显著高于水提醇沉后所得多糖（P<0.05）。结论 低共熔溶剂作为提取介质具有高效和绿色的优点，可用于千斤拔多糖的提取，同时本结果为DESs提取中药活性成分提供新思路。 |
| 关键词: 低共熔溶剂 响应面法 千斤拔 多糖 |
| DOI：10.3969/j.issn.1674-070X.2018.09.008 |
| Received:March 31, 2018 |
| 基金项目:国家发改委标准化项目（ZYBZH-C-HUN-21）；湖南省重大科技专项（2015SK1001）；湖南省教育厅科研优秀青年项目（15B170）；湖南省教育厅科技项目（16C1220）。 |
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| Extraction of Polysaccharides from Moghania macrophylla Based on Deep Eutectic Solvents |
| XIONG Suhui,XIA Bohou,LEI Simin,LI Shihui,XIE Mingxia,LI Yamei,LIN Yan |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Changsha, Hunan 410208, China;Hunan Engineering Laboratory for Prevention and Control Technology of Toxic Substances in Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the extraction of polysaccharides from Moghania macrophylla using new green deep eutectic solvents (DESs) and related extraction process and extraction efficiency. Methods The response surface method was used to optimize the extraction process for polysaccharides from Moghania macrophylla, and the water extraction and alcohol precipitation method was used to evaluate the extraction efficiency of DES. Results The DESs composed of choline chloride and 1,3-butanediol was most suitable for the extraction of polysaccharides from Moghania macrophylla. After optimization with the response surface method, an extraction temperature of 70℃, a water ratio of 11%, an extraction time of 41 minutes, and a liquid-solid ratio of 11 ml/g achieved a yield of polysaccharides from Moghania macrophylla of 2.47%, which was significantly higher than the yield obtained by water extraction and alcohol precipitation (P<0.05). Conclusion DESs has the advantages of high efficiency and being environment-friendly as an extraction medium and can be used for the extraction of polysaccharides from Moghania macrophylla. Meanwhile, the results of this study provides new ideas for the use of DESs in the extraction of active components of traditional Chinese medicine. |
| Key words: deep eutectic solvent response surface method Moghania macrophylla polysaccharide |
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