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李琳霈,杨晓,潘博,谭小宁,罗吉,蒋益兰.疏肝健脾解毒方对人乳腺癌细胞MCF-7增殖及凋亡的影响[J].湖南中医药大学学报英文版,2018,38(6):645-649.[Click to copy
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| 疏肝健脾解毒方对人乳腺癌细胞MCF-7增殖及凋亡的影响 |
| 李琳霈,杨晓,潘博,谭小宁,罗吉,蒋益兰 |
| (湖南省中医药研究院附属医院肿瘤科, 湖南 长沙 410006;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 研究中药疏肝健脾解毒方对人乳腺癌细胞MCF-7增殖及凋亡的影响。方法 采用CCK8法检测药物干预12 h、24 h、48 h后对MCF-7细胞增殖的影响（分为空白对照组、阳性药物对照组及不同浓度中药组）；采用流式细胞术检测药物干预36 h及48 h后对细胞晚期凋亡率的影响（分为空白对照组、中药组及阳性药物对照组）。结果 中药组吸光度低于空白组，差异有统计学意义（P<0.05），且随药物浓度增高，吸光度逐渐降低；12 h至24 h，随着作用时间延长，吸光度亦逐渐降低；24 h后较高浓度中药组吸光度与阳性对照组间差异无统计学意义（P>0.05）。与空白组比较，中药组细胞晚期凋亡率较高，差异有显著统计学意义（P<0.01），但中药组细胞凋亡率低于阳性对照组，差异有显著统计学意义（P<0.01）。结论 疏肝健脾解毒方对人乳腺癌细胞MCF-7增殖有一定的抑制作用，可能通过诱导细胞凋亡实现。 |
| 关键词: 疏肝健脾解毒方 人乳腺癌细胞MCF-7 细胞增殖 细胞凋亡 |
| DOI：10.3969/j.issn.1674-070X.2018.06.008 |
| Received:March 15, 2017 |
| 基金项目:湖南自然科学基金项目（2016JJ6082），湖南省中医药管理局重点项目（201634）。 |
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| Effect of Shugan Jianpi Jiedu Decoction on Proliferation and Apoptosis of Human Breast Cancer Cell Line MCF-7 |
| LI Linpei,YANG Xiao,PAN Bo,TAN Xiaoning,LUO Ji,JIANG Yilan |
| (Department of Oncology, Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine, Changsha, Hunan 410006, China;Grade 2016 Docteral Candidate, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To study the effect of Shugan Jianpi Jiedu decoction on proliferation and apoptosis of human breast cancer cell line MCF-7. Methods The MCF-7 cells were intervened with drugs for 12 h, 24 h and 48 h (assigned into blank control group, positive drug control group and different concentrations of TCM groups), then MCF-7 cells proliferation was detected by CCK8 method. The apoptosis rate of the cells was measured by flow cytometry after intervention with drugs for 36 h and 48 h. Results The absorbance of TCM groups was lower than that of the blank group, the difference was statistically significant (P<0.05). The absorbance decreased gradually with the increase of drug concentration, and reduced gradually with the extension of time from 12 h to 24 h, while after 24 h, the absorbance between the high concentration TCM group and the positive control group was not statistically significant (P>0.05). The cell apoptosis rate in TCM groups was higher than that in blank group, the difference was statistically significant (P<0.01), but was significantly lower than that in positive group (P<0.01). Conclusion Shugan Jianpi Jiedu decoction exhibits certain inhibitory effect on human breast cancer cell MCF-7 proliferation, and its mechanism maybe through inducing cell apoptosis. |
| Key words: Shugan Jianpi Jiedu decoction human breast cancer cell line MCF-7 cell proliferation cell apoptosis |
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