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彭俊,潘坤,刘峥嵘,秦裕辉,彭清华.双丹明目胶囊对糖尿病模型大鼠视网膜VEGF-a、VEGF-b表达的影响[J].湖南中医药大学学报英文版,2018,38(6):635-640.[Click to copy
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| 双丹明目胶囊对糖尿病模型大鼠视网膜VEGF-a、VEGF-b表达的影响 |
| 彭俊,潘坤,刘峥嵘,秦裕辉,彭清华 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察双丹明目胶囊对糖尿病模型大鼠视网膜VEGF-a、VEGF-b表达的影响。方法 将40只SD大鼠，采用随机数字表法分为A（正常组）、B（模型组）、C（双丹明目组）、D（阳性对照组）4组，每组10只20眼。将B、C、D三组实验大鼠采用STZ（以50 mg/kg的剂量）一次性尾静脉注射建立糖尿病视网膜病变模型，造模1周后开始连续灌胃用药，灌胃后4周、8周，分别处死一半动物，采用免疫组化法检测视网膜组织中VEGF-a、VEGF-b的表达。结果 成模后用药第4周、8周，模型组、双丹明目组、阳性对照组视网膜中VEGF-a、VEGF-b蛋白表达平均灰度值均低于正常组，平均光密度值均高于正常组，其中模型组与正常组比较，差异均具有统计学意义（P<0.01）；双丹明目组、阳性对照组VEGF-a、VEGF-b表达的平均灰度值均高于模型组、平均光密度值均低于模型组（P<0.05或P<0.01）。结论 双丹明目胶囊能明显提高糖尿病模型大鼠视网膜中VEGF-a、VEGF-b表达的平均灰度值、降低其平均光密度值，表明双丹明目胶囊能明显降低VEGF-a、VEGF-b在视网膜中的表达。 |
| 关键词: 双丹明目胶囊 糖尿病大鼠模型 VEGF-a VEGF-b |
| DOI：10.3969/j.issn.1674-070X.2018.06.006 |
| Received:April 04, 2018 |
| 基金项目:国家自然科学基金面上项目（81473737）；湖南省研究生科研创新项目（CX2016B377，CX2017B432）；中医药防治五官科疾病湖南省重点实验室建设项目（2017TP1018）；长沙市科技计划项目（kc1704005）；中央财政支持地方高校建设项目；国家中医药管理局中医眼科学重点学科建设项目；湖南省中医五官科学重点学科建设项目。 |
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| Effect of Shuangdan Mingmu Capsule on the Expression of VEGF-a, VEGF-b in Retina of Diabetic Rat Models |
| PENG Jun,PAN Kun,LIU Zhengrong,QIN Yuhui,PENG Qinghua |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of Shuangdan Mingmu capsule on the expression of VEGF-a and VEGF-b in retina of diabetic model rats. Methods The 40 SD rats were randomly divided into group A (normal group), group B (model group), group C (Shuangdan Mingmu group) and group D (positive control group), 10 rats (20 eyes) in each group. The diabetic model rats were built by one-time tail vein injection with STZ (50 mg/kg). After modeling for one week, the rats were given medicine by gavage. After gavage for 4 weeks and 8 weeks, half animals were sacrificed. The expression of VEGF-a and VEGF-b in retina were deected by immunohistochemical method. Results After modeling for 4 weeks and 8 weeks, the average gray value of VEGF-a and VEGF-b expression in the retina of the model group, Shuangdan Mingmu group and the positive control group were lower than those of the normal group, and the average optical density of those three groups were higher than the normal group. There was a significant difference between the model group and the normal group (P<0.01). The average gray value of VEGF-a and VEGF-b expression of Shuangdan Mingmu group and positive control group were higher than those of model group, and the average optical density value were lower than those of model group (P<0.05 or P<0.01). Conclusion Shuangdan Mingmu capsule ccould significantly increase the average gray level of VEGF-a and VEGF-b in diabetic retina and decrease its average optical density. It indicates that Shuangdan Mingmu capsule could significantly reduce the expression of VEGF-a and VEGF-b in the retina. |
| Key words: Shuangdan Mingmu Capsule diabetic rat model VEGF-a VEGF-b |
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