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欧阳里知,谭静,赵欢,彭卓隽,戴丽雯,陈欲攀,李铁浪,林亚平.艾灸对荷瘤胃癌大鼠外周血中CD3-CD161+NK与CD3+CD161+NKT含量的影响[J].湖南中医药大学学报英文版,2018,38(5):541-545.[Click to copy
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| 艾灸对荷瘤胃癌大鼠外周血中CD3-CD161+NK与CD3+CD161+NKT含量的影响 |
| 欧阳里知,谭静,赵欢,彭卓隽,戴丽雯,陈欲攀,李铁浪,林亚平 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨艾灸对荷瘤模型大鼠外周血中CD3-CD161+NK （Nature kill cell，自然杀伤细胞）、CD3+CD161+NKT （Nature kill T cell，自然杀伤T细胞）含量的影响。方法 50只健康SD大鼠，雌雄各半，随机分为空白组、假手术组、模型组、艾灸组、红外组5组，每组10只。采用Walker-256细胞株来源的瘤组织原位种植法制备胃癌模型。造模成功后艾灸组进行中脘、足三里等穴位悬灸，红外组进行腹背部胃体表投影区域的短波红外线照射，每次20 min，每日1次，连续21 d。期间密切观察大鼠行为学变化，记录体质量、饮食、饮水情况。第22天，各组大鼠禁食不禁水12 h后，眼眶采血，流式细胞法测外周血中CD3-CD161+NK、CD3+CD161+NKT含量变化。结果 与空白组比较，模型组大鼠成模后体质量、食量明显降低，外周血中CD3+CD161+NKT增加（P<0.05）。与模型组比较，艾灸组大鼠干预中、后期体质量，食量增加，外周血中CD3-CD161+NK、CD3+CD161+NKT增加（P<0.05）。与红外组比较，艾灸组大鼠体质量、食量、外周血中CD3-CD161+NK、CD3+CD161+NKT增加更明显（P<0.05）。结论 艾灸能增加胃癌SD大鼠体质量，恢复食量，改善胃癌大鼠状态，其机制可能与外周血中CD3-CD161+NK、CD3+CD161+NKT含量增加，细胞免疫增强有关。 |
| 关键词: 胃癌 艾灸 模型大鼠 CD3-CD161+NK CD3+CD161+NKT |
| DOI：10.3969/j.issn.1674-070X.2018.05.014 |
| Received:December 24, 2017 |
| 基金项目:国家自然科学基金面上项目（81574077）。 |
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| Effect of Moxibustion on CD3-CD161+ NK and CD3+CD161+ NKT in Peripheral Blood of Tumor Bearing Gastric Carcinoma Rats |
| OUYANG Lizhi,TAN Jing,ZHAO Huan,PENG Zhuojun,DAI Liwen,CHEN Yupan,LI Tielang,LIN Yaping |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To discuss the effect of moxibustion on CD3-CD161+NK and CD3+CD161+ NKT in peripheral blood of tumor bearing gastric carcinoma rats. Methods 50 healthy SD rats were randomly divided into 5 groups:blank group, control group, model group, moxibustion group and infrared group, 10 rats in each group, half male and half female. Walker-256 cells were used to prepare gastric carcinoma model by situ implantation of tumor tissue. Moxibustion group was given suspended moxibustion at zhongwan (CV12), Zusanli (ST36). The infrared group was given short wave infrared radiation for 20 minutes on the stomach and lower back, once daily. The changes of behavior were observed, and body mass, diet and drinking water were recorded. At the 22nd day, after fasting for 12 hours, orbital blood of rats were collected. The changes of CD3-CD161+ NK and CD3+CD161+ NKT in peripheral blood were determined by flow cytometry instrument. Results Compared with blank group, the body mass and eating capacity in model group decreased significantly (P<0.05), and CD3+CD161+ NKT in peripheral blood increased (P<0.05). Compared with model group, quality and eating capacity of rats in later period treatment improved in moxibustion group obviously (P<0.05), and CD3-CD161+NK, CD3+CD161+ NKT in peripheral blood increased (P<0.05). Compared with infrared group, the improvement of body mass and eating capacity was more obvious in moxibustion group, and CD3-CD161+NK, CD3+CD161+ NKT increased in peripheral blood (P<0.05). Conclusion Moxibustion could increase body mass, recover eating capacity, and improve state of SD rats with gastric carcinoma. The mechanism may be related to the increase of CD3-CD161+ NK and CD3+CD161+ NKT in peripheral blood and enhancement of NK cell immunization. |
| Key words: gastric carcinoma moxibustion model rats CD3-CD161+ NK CD3+CD161+ NKT |
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