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林静,张洁,陈燕.补肾药膳方对绝经后骨质疏松症模型大鼠CTX-Ⅰ指标及骨组织形态的影响[J].湖南中医药大学学报英文版,2018,38(4):389-392.[Click to copy
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| 补肾药膳方对绝经后骨质疏松症模型大鼠CTX-Ⅰ指标及骨组织形态的影响 |
| 林静,张洁,陈燕 |
| (湖南中医药大学护理学院, 湖南 长沙 410208;湖南省中医药研究院附属医院, 湖南 长沙 410006) |
| 摘要: |
| 目的 观察补肾药膳方对绝经后骨质疏松症（PMOP）模型大鼠Ⅰ型胶原C端肽（CTX-Ⅰ）指标及骨组织形态的影响。方法 SPF级雌性SD大鼠随机分为正常组、假手术组、模型组、补肾药膳方高剂量组、补肾药膳方低剂量组、骨疏康组，每组10只。除正常组、假手术组外，其他组大鼠进行双侧卵巢摘除，适应性饲养15 d后，分组干预12周，ELISA法检测血清中骨代谢标志物CTX-Ⅰ浓度表达情况，HE染色镜下观察骨组织形态。结果 与正常组和假手术组比较，模型组、补肾药膳方高、低剂量组、骨疏康组大鼠血清CTX-Ⅰ浓度均升高（P<0.05）；与模型组比较，补肾药膳方高剂量组、低剂量组、骨疏康组血清CTX-Ⅰ浓度显著降低（P<0.05），低剂量组血清CTX-Ⅰ浓度虽低于高剂量组、骨疏康组，但差异无统计学意义（P>0.05）。在骨形态上，模型组骨小梁明显变细，出现明显断裂，有扭曲，形态结构差，骨小梁间的间隙增大，骨髓腔扩大融合明显，高剂量组、低剂量组与骨疏康组均能一定程度上改善骨形态。结论 补肾药膳方各剂量对CTX-I浓度的影响与骨疏康颗粒疗效相当，一定程度能降低骨吸收标志物水平，减少骨吸收，改善骨形态，缓解骨质疏松，但在剂量精确方面仍可待将来研究。 |
| 关键词: 补肾药膳方 绝经后骨质疏松 CTX-Ⅰ指标 骨形态 |
| DOI：10.3969/j.issn.1674-070X.2018.04.006 |
| Received:March 04, 2018 |
| 基金项目:湖南省科技厅课题（2015SK20168）；湖南中医药大学2017年一方研究生科研创新项目（YF201712）。 |
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| Effect of Reinforcing Kidney Medicinal Diet on CTX-ⅠIndex and Bone Morphology in Postmenopausal Osteoporosis Model Rats |
| LIN Jing,ZHANG Jie,CHEN Yan |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Affiliated Hospital of Hunan Academy of Chinese Medicine, Changsha, Hunan 410006, China) |
| Abstract: |
| Objective To observe the effect of reinforcing kidney medicinal diet on CTX-Ⅰindex and bone morphological changes in postmenopausal osteoporosis (PMOP) model rats. Methods SPF female SD rats were randomly divided into normal group, sham operation group, model blank group, high dose group, low dose group and Gushukang group, with 10 rats in each group. Except normal group and sham operation group, other rats were ovariectomized. After 15 days of adaptive feeding, the rats were given intervention treatment for 12 weeks. The expression of CTX-Ⅰ in serum was detected by ELISA method. Bone morphology was observed by HE staining. Results Compared with the normal group and sham-operation group, the concentration of CTX-Ⅰin model blank group, high dose group, low dose group, Gushukang group increased significantly (P<0.05). Compared with the model blank group, the concentration of CTX-Ⅰ in high dose group, low dose group and Gushukang group decreased significantly (P<0.05). CTX-1 in low dose group was lower than the high dose group and Gushukang group, but the difference between them was not significant(P>0.05). In the bone morphology, the trabecular bone of the model blank group was significantly thinner, with obvious fracture, twisting, poor shape and structure, increased gap between the trabecular bone and bone marrow cavity. The bone marrow cavity expanded and merged obviously. The high dose group, the low dose group and the Gushukang group could improve bone morphology to some extent. Conclusion The effect of reinforcing kidney medical diet on the concentration of CTX-I is comparable with that of Gushukang granules. It could reduce the level of bone resorption markers, reduce bone resorption, improve bone shape and alleviate osteoporosis to a certain extent. The accuracy of dose should be further studied. |
| Key words: rreinforcing kidney medicinal diet postmenopausal osteoporosis CTX-Ⅰindex bone morphology |
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